【摘要】：淋巴細(xì)胞主要包括T淋巴細(xì)胞和B淋巴細(xì)胞,分別參與細(xì)胞免疫和體液免疫。本研究應(yīng)用流式細(xì)胞術(shù),以SPF雞為研究對(duì)象,分別在1、4、7、14、21、35、56和90日齡采取雞的法氏囊、胸腺、脾臟以及血液,檢測(cè)其在正常發(fā)育過程中T、B淋巴細(xì)胞數(shù)量的變化情況。通過用流式細(xì)胞儀檢測(cè)B淋巴細(xì)胞中Bu-1b以及IgA+、IgG+和IgM+細(xì)胞數(shù)量的變化情況,結(jié)果發(fā)現(xiàn):1日齡時(shí)Bu-1b細(xì)胞數(shù)量較少,4日齡時(shí)免疫器官及外周血液中的Bu-1細(xì)胞數(shù)量迅速增多,7日齡時(shí),法氏囊中的Bu-1b細(xì)胞達(dá)到增長(zhǎng)的高峰,說明在4至7日齡時(shí)體液免疫功能增強(qiáng);在脾臟、胸腺、法氏囊以及血液中,B淋巴細(xì)胞中的免疫球蛋白主要以IgM+細(xì)胞數(shù)量為主,Ig G+細(xì)胞數(shù)量最少;此外研究還發(fā)現(xiàn),免疫器官及外周血液中的B淋巴細(xì)胞的數(shù)量在35日齡基本達(dá)到穩(wěn)定。通過對(duì)T細(xì)胞數(shù)量的變化進(jìn)行檢測(cè),結(jié)果顯示:在1至4日齡時(shí)CD3+細(xì)胞數(shù)量迅速增多,7日齡時(shí)法氏囊中CD3+細(xì)胞數(shù)量減少,到35日齡以后CD3+細(xì)胞數(shù)量變化幅度不大,基本趨于穩(wěn)定;14日齡時(shí),血液和胸腺中的CD4+細(xì)胞含量多于CD8+細(xì)胞含量,而在21日齡時(shí)法氏囊中的CD4+細(xì)胞含量多于CD8+細(xì)胞的含量。在21日齡時(shí)CD4+細(xì)胞數(shù)量趨于穩(wěn)定。本研究利用流式細(xì)胞術(shù)研究SPF雞在正常生理情況下,免疫器官和血液中T、B淋巴細(xì)胞及其亞群中細(xì)胞數(shù)量的變化規(guī)律,從而了解到雞出殼后的免疫狀態(tài),這將為適時(shí)免疫和疾病監(jiān)測(cè)提供了重要的理論依據(jù)。
[Abstract]:Lymphocytes mainly include T lymphocytes and B lymphocytes, which participate in cellular immunity and humoral immunity respectively. In this study, flow cytometry was used to investigate the changes of T B lymphocytes in the bursa of Fabricius, thymus, spleen and blood of SPF chickens during normal development. The changes of Bu-1b, IgA, IgG and IgM cells in B lymphocytes were detected by flow cytometry. The results showed that the number of Bu-1b cells was less at 1 day old. The number of Bu-1 cells in immune organs and peripheral blood increased rapidly at the age of 4 days, and the Bu-1b cells in the bursa of Fabricius reached the peak at the age of 7 days, indicating the enhancement of humoral immune function at the age of 4 to 7 days. In spleen, thymus, bursa of Fabricius and blood, the number of immunoglobulin in B lymphocytes was mainly IgM cells, mainly, Ig G cells. In addition, the number of B lymphocytes in immune organs and peripheral blood was stable at 35 days old. The results showed that the number of CD3 cells increased rapidly at the age of 1 to 4 days, the number of CD3 cells in the bursa of Fabricius decreased at the age of 7 days, and the number of CD3 cells did not change significantly after 35 days of age. It tends to be stable; At the age of 14 days, the content of CD4 cells in blood and thymus was higher than that of CD8 cells, and the content of CD4 cells in bursa of Fabricius was higher than that of CD8 cells in the bursa of Fabricius at 21 days of age. At 21 days of age, the number of CD4 cells tended to stabilize. In this study, flow cytometry was used to study the changes of the number of TG-B lymphocytes and their subsets in the immune organs and blood of SPF chickens under normal physiological conditions. This will provide an important theoretical basis for timely immunization and disease surveillance.
【學(xué)位授予單位】：吉林農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S831

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本文編號(hào)：2421901