【摘要】：試驗旨在探討無血清條件下臍帶間充質(zhì)干細胞(UC-MSCs)和奶牛乳腺上皮細胞(BMECs)共培養(yǎng)對類胰島素樣生長因子-Ⅰ(IGF-Ⅰ)表達的影響,將UC-MSCs和BMECs按1∶2直接共培養(yǎng),對比單純培養(yǎng)的兩種細胞,48h時利用ELISA法檢測各組上清IGF-Ⅰ濃度水平,再利用Transwell小室將兩種細胞分離培養(yǎng),實時熒光定量PCR檢測各組細胞IGF-ⅠR、IGF-ⅠmRNA的表達。結(jié)果顯示,共培養(yǎng)中IGF-Ⅰ濃度顯著高于UC-MSCs組(P0.05),極顯著高于BMECs組(P0.01);UC-MSCs/BMECs、BMECs/UC-MSCs組IGF-ⅠmRNA表達值均極顯著高于單純培養(yǎng)組(P0.01),BMECs/UC-MSCs組顯著高于UC-MSCs/BMECs組(P0.05);UC-MSCs/BMECs、BMECs/UC-MSCs組IGF-ⅠR mRNA表達值顯著或極顯著高于單純培養(yǎng)組(P0.05;P0.01),BMECs/UC-MSCs組較UCMSCs/BMECs組差異顯著(P0.05)。綜上,體外無血清培養(yǎng)條件下,UC-MSCs和BMECs共培養(yǎng)可顯著提高IGF-ⅠR及IGF-ⅠmRNA表達,IGF-Ⅰ濃度水平和IGF-ⅠR mRNA的表達具有一致性,IGF-Ⅰ主要存在于UCMSCs中。
[Abstract]:The aim of this study was to investigate the effect of co-culture of umbilical cord mesenchymal stem cells (UC-MSCs) and bovine mammary epithelial cells (BMECs) on the expression of insulin-like growth factor- 鈪，

本文編號：2411103