【摘要】：現(xiàn)代肉羊生產(chǎn)體系就是要以適繁母羊?yàn)榛A(chǔ),充分發(fā)揮其繁殖潛力,以先進(jìn)的科學(xué)技術(shù)手段為依托,提高其繁殖力。而寧夏灘羊繁殖還停留在傳統(tǒng)的自然交配,母羊繁殖力低,國(guó)內(nèi)外重要的先進(jìn)技術(shù)手段的價(jià)值沒(méi)有得到應(yīng)有的發(fā)揮和體現(xiàn),同期發(fā)情、人工授精等繁殖技術(shù)沒(méi)有得以廣泛推廣。本試驗(yàn)針對(duì)寧夏灘羊養(yǎng)殖現(xiàn)狀,對(duì)寧夏灘羊進(jìn)行同期發(fā)情技術(shù)和精液低溫保存技術(shù)的應(yīng)用性研究。通過(guò)不同的同期發(fā)情處理方案,研究出效果最佳的同期發(fā)情方法;通過(guò)對(duì)不同配方低溫保存稀釋液對(duì)公羊精液保存效果的研究,篩選出低溫條件下保存灘羊精液的最佳稀釋劑配方;通過(guò)同期發(fā)情技術(shù)、發(fā)情鑒定、人工采精、精液稀釋保存技術(shù)、人工授精技術(shù)以及妊娠診斷技術(shù)等一系列繁殖技術(shù)的綜合應(yīng)用,探索提高灘羊繁殖力的技術(shù)方法。本試驗(yàn)的主要研究結(jié)果如下:1.使用“陰道栓+FSH+PG”處理的試驗(yàn)組羊的同期發(fā)情率和配種受胎率均高于“PG+FSH”處理的試驗(yàn)組(P0.05),注射FSH 50 IU+PG 0.1 mg 96 h后放置NRID處理13 d,分別在-24 h,-12 h注射FSH 50 IU,0 h去栓并注射PG 0.1 mg的同期發(fā)情方案效果最佳,使母羊同期發(fā)情率達(dá)到100%,極顯著高于其他試驗(yàn)組(P0.01);母羊情期受胎率達(dá)到75.3%,顯著高于其他各組(P0.05),且發(fā)情時(shí)間相對(duì)集中于24~48 h。2.試驗(yàn)所選用的6種稀釋液配方以5號(hào)保存效果最好,保存時(shí)間和有效存活時(shí)間及生存指數(shù)顯著高于其他配方(P0.05),6號(hào)次之。用5號(hào)稀釋液保存精液,使灘羊精液最長(zhǎng)存活162 h,精子有效存活時(shí)間長(zhǎng)達(dá)87±5 h,生存指數(shù)89.64±5.1,24 h內(nèi)配種受胎率50%以上。3.精液稀釋液中添加海藻糖能夠顯著提高精子活率、精子運(yùn)動(dòng)性能以及精子質(zhì)膜完整性(P0.05)。在本試驗(yàn)所設(shè)計(jì)的濃度范圍內(nèi),隨海藻糖濃度增加,精子活率并無(wú)顯著變化(P0.05),精子質(zhì)膜完整率和精子運(yùn)動(dòng)性能均呈成上升趨勢(shì),但這種變化趨勢(shì)不顯著(P0.05)。相同保存時(shí)間下,濃度為0.03 g/mL時(shí)質(zhì)膜完整率比添加其他濃度時(shí)高,差異不顯著(P0.05),精子運(yùn)動(dòng)速度也高于其他添添加濃度,差異不顯著(P0.05)。
[Abstract]:The production system of modern meat sheep is to make full use of its breeding potential and to improve its reproduction ability based on advanced scientific and technological means. However, the breeding of Ningxia Tan sheep still stays in the traditional natural mating, the fecundity of the ewes is low, the value of the important advanced technical means at home and abroad has not been given full play and embodiment, and the breeding techniques such as simultaneous estrus and artificial insemination have not been widely popularized. Based on the present situation of Ningxia Tan sheep breeding, the application of synchronized estrus and semen cryopreservation technique was studied. Through different estrus synchronization treatment schemes, the best estrus synchronization method was developed. The best diluent formula for the preservation of Tan sheep semen at low temperature was obtained by studying the effects of different low temperature preservation diluents on the preservation of ram semen. Through the comprehensive application of a series of breeding techniques, such as simultaneous estrus technology, estrus identification, artificial semen collection, semen dilution and preservation technology, artificial insemination technology and pregnancy diagnosis technology, this paper explores the technical methods to improve the fecundity of Tan sheep. The main results of this study are as follows: 1. The rate of synchronous estrus and fecundity of sheep treated with "vaginal suppository FSH PG" was higher than that of "PG FSH" treatment group (P0.05). The treatment group was treated with NRID for 13 days after injecting FSH 50 IU PG 0.1 mg for 96 h, and was treated with NRID for -24 h, respectively. The estrus synchronization rate of estrus was 100, which was significantly higher than that of other experimental groups (P0.01). The conception rate of estrus in estrus was 75.3, which was significantly higher than that in other groups (P0.05), and the estrus time was relatively concentrated at 244h.2. The six diluent formulations selected in the experiment had the best preservation effect, and the preservation time, effective survival time and survival index were significantly higher than those of other formulations (P0.05), followed by No. 6. The semen of Tan Sheep was preserved with dilution No. 5 for the longest time of 162 hours, the effective survival time of sperm was 87 鹵5 h, and the survival index was 89.64 鹵5.1 鈩，

本文編號(hào)：2410642