仔豬A型口蹄疫母源抗體及細(xì)胞因子消長(zhǎng)規(guī)律
發(fā)布時(shí)間：2018-12-26 15:54
【摘要】：口蹄疫(Foot-and-mouth disease,FMD)是由口蹄疫病毒(Foot-and-mouth disease virus,FMDV)感染引起的偶蹄動(dòng)物的一種急性、熱性、高度接觸性傳染病。該病多次在世界范圍內(nèi)爆發(fā)流行,造成巨大政治、經(jīng)濟(jì)損失。新生仔豬有自己的免疫系統(tǒng),但不成熟,不能充分抵抗病原體感染。而母源抗體則為仔豬提供了早期保護(hù)作用。但母源抗體具有兩面性,即能保護(hù)仔豬免受感染,又具有干擾疫苗免疫的作用。因此,探索豬口蹄疫母源抗體的傳遞方式,明確母源抗體在仔豬體內(nèi)的消長(zhǎng)規(guī)律,對(duì)于合理制定仔豬口蹄疫疫苗免疫程序具有重要意義。1.采用液相阻斷ELISA和病毒中和試驗(yàn)檢測(cè)5頭母豬和25頭仔豬血清中A型口蹄疫抗體水平。結(jié)果表明,仔豬采食初乳前血清中幾乎不存在A(yíng)型口蹄疫抗體,采食初乳1d后抗體水平達(dá)到峰值并在5~7d內(nèi)維持穩(wěn)定,而后逐漸降低,證明母源抗體是通過(guò)初乳傳遞給仔豬的。根據(jù)抗體水平和消減速度確定,母豬抗體水平為1:1024、1:720和1:256時(shí),仔豬最宜首免時(shí)間分別為56、42和28日齡左右。2.采用ELISA檢測(cè)分析母豬血清、乳汁及對(duì)應(yīng)仔豬血清和臍帶血中IgG、IgA、IgM的含量變化趨勢(shì)。結(jié)果表明,母豬血清和初乳中3種免疫球蛋白含量豐富,仔豬采食初乳前的血清和臍帶血中不含IgG、IgA、IgM,仔豬采食初乳1d后血清中IgG、IgM、IgA含量迅速增加,然后IgA和IgG含量呈連續(xù)下降趨勢(shì),而IgM含量持續(xù)下降至42d左右又會(huì)大幅度回升。3.采用抗體芯片QAP-CYT-1和QAP-CYT-2檢測(cè)母豬血清、乳汁及對(duì)應(yīng)仔豬血清中20種細(xì)胞因子。結(jié)果表明,母豬血清和乳汁中20種細(xì)胞因子含量豐富;其中8種細(xì)胞因子(TNFa、TGFb1、IFNg、GM-CSF、IL-10、IL-6、IL-4、IL-1b)在仔豬采食初乳前的血清中未檢出,但在采食初乳后含量大幅度升高,說(shuō)明這8種細(xì)胞因子是通過(guò)初乳被動(dòng)傳遞的;其余12種細(xì)胞因子(CCL3L1、IFNα、IL-1α、IL-1ra、IL-13、IL-17A、MIG、IL-18、MIP-1b、PECEM-1、IL-8、IL-12P40P70)在仔豬采食初乳前的血清中就已經(jīng)存在,仔豬采食初乳后其含量增加,說(shuō)明這12種細(xì)胞因子也可以通過(guò)初乳傳遞給仔豬,但是否通過(guò)胎盤(pán)傳遞,尚需進(jìn)一步證明。
[Abstract]:Foot and mouth disease (Foot-and-mouth disease,FMD) is an acute, feverish and highly contagious disease in cloven-hoofed animals caused by foot-and-mouth disease virus (Foot-and-mouth disease virus,FMDV). The disease broke out many times in the world, causing huge political and economic losses. Newborn piglets have their own immune system, but not mature enough to resist pathogen infection. Maternal antibodies provide early protection for piglets. But maternal antibody has two sides, it can protect piglets from infection and interfere with vaccine immunity. Therefore, it is of great significance to explore the transmission mode of maternal antibody against foot-and-mouth disease (FMD) in piglets and to clarify the rules of maternal antibody growth and decline in piglets, which is of great significance for the rational formulation of immunization procedures for foot-and-mouth disease vaccine in piglets. The level of FMD type A antibody in serum of 5 sows and 25 piglets was detected by liquid phase blocking ELISA and virus neutralization test. The results showed that there was almost no type A foot-and-mouth disease antibody in the serum of piglets before feeding colostrum, and the antibody level reached the peak level after 1 day of feeding colostrum and remained stable within 5 ~ 7 days, but gradually decreased. It was proved that maternal antibody was transmitted to piglets through colostrum. According to the antibody level and the subtractive speed, when the antibody level of sows was 1: 1024, 1: 720 and 1: 256, the optimal first immunity time of piglets was about 5642 and 28 days old, respectively. The changes of IgG,IgA,IgM content in serum, milk and corresponding piglets and umbilical cord blood were analyzed by ELISA. The results showed that the three kinds of immunoglobulin were abundant in serum and colostrum of sows, and the IgG,IgM,IgA content in serum and cord blood of piglets before feeding colostrum and in cord blood without IgG,IgA,IgM, increased rapidly after feeding colostrum for 1 day. Then the content of IgA and IgG decreased continuously, and the content of IgM continued to decrease to 42 days. QAP-CYT-1 and QAP-CYT-2 were used to detect 20 cytokines in sows' serum, milk and corresponding piglets. The results showed that 20 cytokines were abundant in serum and milk of sows. Eight kinds of cytokines (TNFa,TGFb1,IFNg,GM-CSF,IL-10,IL-6,IL-4,IL-1b) were not detected in the serum of piglets before feeding colostrum, but the contents increased significantly after feeding colostrum. These eight cytokines were transmitted passively through colostrum. The other 12 cytokines (CCL3L1,IFN 偽, IL-1 偽, IL-1ra,IL-13,IL-17A,MIG,IL-18,MIP-1b,PECEM-1,IL-8,IL-12P40P70) were already present in the serum of piglets before feeding colostrum. These 12 cytokines can also be transmitted to piglets through colostrum, but whether they are transmitted through placenta or not needs further proof.
【學(xué)位授予單位】：甘肅農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：S858.28
[Abstract]:Foot and mouth disease (Foot-and-mouth disease,FMD) is an acute, feverish and highly contagious disease in cloven-hoofed animals caused by foot-and-mouth disease virus (Foot-and-mouth disease virus,FMDV). The disease broke out many times in the world, causing huge political and economic losses. Newborn piglets have their own immune system, but not mature enough to resist pathogen infection. Maternal antibodies provide early protection for piglets. But maternal antibody has two sides, it can protect piglets from infection and interfere with vaccine immunity. Therefore, it is of great significance to explore the transmission mode of maternal antibody against foot-and-mouth disease (FMD) in piglets and to clarify the rules of maternal antibody growth and decline in piglets, which is of great significance for the rational formulation of immunization procedures for foot-and-mouth disease vaccine in piglets. The level of FMD type A antibody in serum of 5 sows and 25 piglets was detected by liquid phase blocking ELISA and virus neutralization test. The results showed that there was almost no type A foot-and-mouth disease antibody in the serum of piglets before feeding colostrum, and the antibody level reached the peak level after 1 day of feeding colostrum and remained stable within 5 ~ 7 days, but gradually decreased. It was proved that maternal antibody was transmitted to piglets through colostrum. According to the antibody level and the subtractive speed, when the antibody level of sows was 1: 1024, 1: 720 and 1: 256, the optimal first immunity time of piglets was about 5642 and 28 days old, respectively. The changes of IgG,IgA,IgM content in serum, milk and corresponding piglets and umbilical cord blood were analyzed by ELISA. The results showed that the three kinds of immunoglobulin were abundant in serum and colostrum of sows, and the IgG,IgM,IgA content in serum and cord blood of piglets before feeding colostrum and in cord blood without IgG,IgA,IgM, increased rapidly after feeding colostrum for 1 day. Then the content of IgA and IgG decreased continuously, and the content of IgM continued to decrease to 42 days. QAP-CYT-1 and QAP-CYT-2 were used to detect 20 cytokines in sows' serum, milk and corresponding piglets. The results showed that 20 cytokines were abundant in serum and milk of sows. Eight kinds of cytokines (TNFa,TGFb1,IFNg,GM-CSF,IL-10,IL-6,IL-4,IL-1b) were not detected in the serum of piglets before feeding colostrum, but the contents increased significantly after feeding colostrum. These eight cytokines were transmitted passively through colostrum. The other 12 cytokines (CCL3L1,IFN 偽, IL-1 偽, IL-1ra,IL-13,IL-17A,MIG,IL-18,MIP-1b,PECEM-1,IL-8,IL-12P40P70) were already present in the serum of piglets before feeding colostrum. These 12 cytokines can also be transmitted to piglets through colostrum, but whether they are transmitted through placenta or not needs further proof.
【學(xué)位授予單位】：甘肅農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：S858.28
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