發(fā)布時間：2018-12-21 21:01

【摘要】：新城疫防控一直是養(yǎng)雞業(yè)疫病防控的一項重要任務。針對現(xiàn)有新城疫疫苗的出現(xiàn)的效價不夠強,持續(xù)作用時間不夠長等問題,研究和開發(fā)一種安全可靠兼生產(chǎn)推廣成本低的輔助物質(zhì)一直是該領(lǐng)域研究的熱點。本試驗旨在通過選用四種人工合成小肽,在接種新城疫疫苗的同時輔助以飲水的方式給肉雞補飼,以探索不同人工合成小肽對肉雞新城疫疫苗免疫效果的影響,以期篩選出效果最佳的人工合成小肽及其最適使用劑量,為人工合成小肽在實際生產(chǎn)中的科學使用提供依據(jù)。主要研究內(nèi)容如下:1.四種人工合成小肽制劑對新城疫疫苗免疫肉雞的效果研究為研究人工合成小肽對肉雞血清中雞新城疫疫苗抗體效價的影響,將900羽1日齡AA肉雞隨機平均分配到空白對照組、新城疫疫苗組、新城疫疫苗+T4-3H4組、新城疫疫苗+T4-3T4組、新城疫疫苗+3H4組和新城疫疫苗+3T4組,每組設3個重復,每個重復50羽。每組雞分別自由飲用含不同人工合成小肽的飲用水,在免疫后的第0、7、14、21、28和35天分別從每個重復組中隨機挑選2只雞采集血液樣品,測定血清中的雞新城疫疫苗抗體水平。結(jié)果表明:以飲水方式給肉雞飼喂四種人工合成小肽制劑,可以有效提高不同日齡雞血清新城疫疫苗的抗體水平,其中3H4人工小肽的效果最佳,由此可見,以飲水方式給肉雞補飼人工合成小肽制劑有助于提高雞新城疫疫苗的免疫效果。2.人工合成小肽3H4的最適使用濃度研究為探索人工合成小肽3H4的最適使用濃度,將270羽1日齡AA肉雞隨機平均分配到高濃度3H4處理組(2ug/150L)、中濃度3H4處理組(1ug/150L)和低濃度3H4處理組(0.5ug/150L),每組設置3個重復組,每個重復30只雞。每組雞分別自由飲用含不同濃度的人工合成小肽(3H4)的飲用水,在免疫后的第0、7、14、21、28和35天分別從每個重復組中隨機挑選2只雞采集血液樣品,測定血清中的雞新城疫疫苗抗體水平。結(jié)果表明:飲用水添加中濃度(1ug/150L)人工合成小肽3H4時的雞新城疫疫苗免疫效果最佳。
[Abstract]:Newcastle disease prevention and control has been an important task of chicken industry epidemic prevention and control. In order to solve the problem that the titer of Newcastle disease vaccine is not strong enough and the duration of action is not long enough, the research and development of a safe and reliable auxiliary substance with low production and popularization cost has been a hot spot in this field. The purpose of this study was to investigate the effect of different synthetic peptides on the immune effect of Newcastle disease vaccine in broilers by selecting four kinds of synthetic peptides and giving them supplementary feeding with drinking water while inoculating Newcastle disease vaccine. In order to screen out the best synthetic small peptide and its optimal dosage, to provide the basis for the scientific use of synthetic small peptide in practical production. The main contents are as follows: 1. Study on the effects of four synthetic small Peptide preparations on Newcastle Disease Vaccine Immunization in Broiler chickens; in order to study the effect of synthetic small peptides on the antibody titer of Newcastle disease vaccine in serum of broilers, 900 1-day-old AA broilers were randomly and equally assigned to the blank control group. Newcastle disease vaccine group, Newcastle disease vaccine T4-3H4 group, Newcastle disease vaccine T4-3T4 group, Newcastle disease vaccine 3H4 group and Newcastle disease vaccine 3T4 group were divided into 3 replicates with 50 replicates per group. The chickens of each group drank freely drinking water containing different synthetic peptides. On the 0th day after immunization, two chickens were randomly selected from each repeated group to collect blood samples, and the antibody level of Newcastle disease vaccine in serum was determined. The results showed that feeding broilers with four synthetic small peptide preparations in drinking water could effectively improve the antibody level of Newcastle disease vaccine in serum of chickens of different days, and the effect of 3H4 artificial small peptide was the best. Supplemental feeding of synthetic small peptide to broilers with drinking water was helpful to improve the immune effect of Newcastle disease vaccine. 2. Study on the optimal concentration of synthetic small Peptide 3H4 in order to explore the optimal concentration of synthetic small Peptide 3H4, 270 AA broilers of 1 day old were randomly assigned to the high concentration 3H4 treatment group (2ug/150L). There were 3 repeats in each group, 30 chickens in each group. The medium concentration 3H4 treatment group (1ug/150L) and the low concentration 3H4 treatment group (0.5ug/150L) were treated with 3 repeats in each group. Drinking water containing different concentrations of synthetic small peptide (3H4) was freely consumed in each group. Two chickens were randomly selected from each repeated group to collect blood samples on the 28th and 35th day after immunization. The antibody level of Newcastle disease vaccine in serum was determined. The results showed that the best immune effect of Newcastle disease vaccine was obtained when drinking water was supplemented with medium concentration (1ug/150L) of small peptide 3H4.
【學位授予單位】：江西農(nóng)業(yè)大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：S858.31

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