【摘要】：為了研究紫竹根水提物(PNR)對(duì)動(dòng)物體內(nèi)外抗氧化的作用,本試驗(yàn)體外調(diào)查了PNR對(duì)二苯基苦基苯肼(DPPH)自由基、羥自由基、超氧陰離子的清除能力,并測(cè)定了其總還原力;以免疫抑制小鼠為試驗(yàn)對(duì)象,測(cè)定心、肝、腎勻漿液中蛋白、超氧化物歧化酶(SOD)、谷胱甘肽過氧化物酶(GSH-Px)、過氧化氫酶(CAT)的活性、總抗氧化能力(T-AOC)和丙二醛(MDA)水平。結(jié)果顯示,PNR在體外抗氧化的4個(gè)指標(biāo)中,尤其是清除超氧陰離子試驗(yàn)中,表現(xiàn)出良好的抗氧化能力。在小鼠體內(nèi)試驗(yàn)中,與模型對(duì)照組相比,PNR高劑量組的SOD、CAT、GSH-Px和TAOC活性顯著增加(P0.05),所有劑量組的MDA水平顯著降低(P0.05)。試驗(yàn)結(jié)果表明,PNR可以有效地清除自由基,具有較強(qiáng)的抗氧化活性。
[Abstract]:In order to study the antioxidation effect of (PNR) on the activity of (DPPH) radical, hydroxyl radical and superoxide anion in vitro and in vivo, the scavenging ability of PNR on (DPPH) radical, hydroxyl radical and superoxide anion was investigated in vitro. The activities of protein, superoxide dismutase (SOD),) glutathione peroxidase (GSH-Px) and catalase (CAT) (CAT) in heart, liver and kidney homogenate were determined in immunosuppressive mice. Total antioxidant capacity (T-AOC) and malondialdehyde (MDA) levels. The results showed that PNR showed good antioxidant activity in the four indexes of antioxidation in vitro, especially in the superoxide anion scavenging test. In vivo test, compared with the model control group, the activities of SOD,CAT,GSH-Px and TAOC in PNR high dose group increased significantly (P0.05), and the MDA level in all dose groups decreased significantly (P0.05). The results show that PNR can effectively scavenge free radicals and has strong antioxidant activity.
【作者單位】： 南京農(nóng)業(yè)大學(xué)動(dòng)物醫(yī)學(xué)院;蘇州市吳江區(qū)農(nóng)業(yè)委員會(huì);
【基金】：江蘇省自然科學(xué)基金(BK2012220) 江蘇高校優(yōu)勢(shì)學(xué)科建設(shè)工程資助項(xiàng)目(PAPD)
【分類號(hào)】：S853.7

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