【摘要】：高精料日糧引起的瘤胃酸中毒已成為影響現(xiàn)代反芻動(dòng)物生產(chǎn)的重要問(wèn)題。瘤胃酸中毒導(dǎo)致的瘤胃上皮受損是加重并引起酸中毒全身癥狀的主要因素。B族維生素?zé)熕嵋驯蛔C實(shí)有緩解高精料應(yīng)激的作用,但其對(duì)瘤胃屏障有何影響尚未見(jiàn)報(bào)道。本研究通過(guò)體內(nèi)試驗(yàn)來(lái)研究高精料日糧中添加煙酸對(duì)綿羊瘤胃發(fā)酵、抗氧化能力及瘤胃上皮細(xì)胞凋亡的影響,以期從分子水平探討煙酸保護(hù)瘤胃上皮、調(diào)控瘤胃酸中毒的機(jī)制。本試驗(yàn)選擇9頭裝有永久性瘺管的綿羊(34±3 kg BW),采用單因素完全隨機(jī)試驗(yàn)設(shè)計(jì),試驗(yàn)分為3組,每組3個(gè)重復(fù)。第Ⅰ組為低精料組(日糧精粗比為2:8),試驗(yàn)期為10 d,包括7 d預(yù)試期和3 d正試期。第Ⅱ組為高精料組(日糧精粗比為8:2),第Ⅲ組為高精料+煙酸組(日糧精粗比為8:2,煙酸添加量為800 mg/kg日糧干物質(zhì))。結(jié)果表明:(1)與低精料組相比,高精料組降低了瘤胃pH值(P0.01),極顯著增加了瘤胃總揮發(fā)性脂肪酸(TVFA)、乙酸、丙酸、丁酸及乳酸的濃度(P0.01)。高精料日糧還極顯著提高了瘤胃內(nèi)NAD+濃度、NADH濃度、NADH/NAD+的比例(P0.01);高精料+煙酸組極顯著提高了瘤胃pH值(P0.01),降低了乙酸、丙酸、丁酸、總揮發(fā)性脂肪酸(TVFA)及乳酸的濃度(P0.01);同時(shí)還極顯著降低了NAD+、NADH的濃度及NADH/NAD+的比例(P0.01)。(2)與低精料組相比,高精料組的過(guò)氧化氫酶(CAT)、總超氧化物岐化酶(T-SOD)、谷胱甘肽過(guò)氧化物酶(GSH-PX)的活性極顯著降低,總抗氧化能力(T-AOC)極顯著下降,丙二醛(MDA)的濃度極顯著提高(P0.01);高精料條件下添加煙酸極顯著提高了CAT、GSH-PX的活性,T-AOC增強(qiáng),降低了MDA的濃度(P0.01),對(duì)T-SOD并無(wú)影響(P0.05)。(3)與低精料組相比,高精料組的綿羊血清中促炎因子IL-1β、IL-6、TNF-α和抗炎因子IL-4、IL-10的濃度均極顯著升高(P0.01),高精料+煙酸組的綿羊血清中促炎因子IL-1β、IL-6、TNF-α和抗炎因子IL-4、IL-10的濃度均極顯著低于高精料組(P0.01)。與低精料組相比,高精料組的瘤胃乳頭長(zhǎng)度、寬度及角質(zhì)層厚度極顯著降低(P0.01),細(xì)胞凋亡指數(shù)極顯著升高(P0.01);高精料+煙酸組綿羊的瘤胃乳頭長(zhǎng)度、寬度及角質(zhì)層高于高精料組(P0.01或P0.05),極顯著降低了細(xì)胞凋亡指數(shù)(P0.01)。(4)與低精料組相比,高精料組綿羊的瘤胃上皮Fas、Bcl-2、Bax、P53上調(diào),Caspase-3、Caspase-8、Caspase-9的mRNA表達(dá)水平極顯著提高(P0.01),高精料條件下添加煙酸極顯著地下調(diào)Fas、Bcl-2、Bax、P53及Caspase-3、Caspase-8、Caspase-9的表達(dá)(P0.01)。綜上所述,高精料應(yīng)激導(dǎo)致綿羊瘤胃內(nèi)VFA和乳酸濃度提高,pH值降低,上皮細(xì)胞抗氧化性能力下降,激活凋亡相關(guān)基因P53、Bcl-2、Bax、Fas表達(dá)上調(diào),Caspase-3、Caspase-8、Caspase-9表達(dá)提高,導(dǎo)致瘤胃上皮細(xì)胞凋亡,瘤胃上皮功能受損。而煙酸一定程度上可以緩解高精料應(yīng)激,提高瘤胃上皮細(xì)胞抗氧化能力,抑制凋亡相關(guān)基因的表達(dá),抑制了瘤胃上皮細(xì)胞的凋亡,保護(hù)了瘤胃上皮結(jié)構(gòu)功能完整。
[Abstract]:Rumen acidosis caused by high concentrate diet has become an important problem affecting the production of modern ruminants. The damage of rumen epithelium caused by rumen acidosis is the main factor that exacerbates and causes the systemic symptoms of acidosis. Group B vitamin nicotinic acid has been proved to have the effect of relieving high concentrate stress, but its effect on rumen barrier has not been reported. In this study, the effects of niacin supplementation on rumen fermentation, antioxidant ability and apoptosis of rumen epithelial cells in sheep were studied in vivo. The aim of this study was to explore the mechanism of niacin in protecting rumen epithelium and regulating rumen acidosis at molecular level. Nine sheep with permanent fistula (34 鹵3 kg BW),) were randomly divided into 3 groups with 3 repeats. The trial period was 10 days, which included 7 days pretest period and 3 days positive trial period. The second group was high concentrate group (the ration was 8:2), the third group was high concentrate niacin group (the ration of concentrate and crude ration was 8: 2, niacin was 800 mg/kg dry matter). The results showed that: (1) compared with the low concentrate group, the high concentrate group decreased the rumen pH value (P0.01), and significantly increased the concentration of (TVFA), acetic acid, propionic acid, butyric acid and lactic acid in the rumen total volatile fatty acids (P0.01). High concentrate diet also significantly increased rumen NAD concentration, NADH concentration, NADH/NAD ratio (P0.01); The concentration of acetic acid, propionic acid, butyric acid, total volatile fatty acid (TVFA) and lactic acid decreased significantly (P0.01) in high concentrate niacin group. At the same time, the concentration of NAD, NADH and the ratio of NADH/NAD (P0.01). (2) were significantly decreased in the high concentrate group compared with the low concentrate group, the catalase (CAT), total superoxide dismutase (T-SOD) in the high concentrate group was significantly lower than that in the low concentrate group. Glutathione peroxidase (GSH-PX) activity, total antioxidant capacity (T-AOC) and malondialdehyde (MDA) concentration were significantly decreased (P0.01). Under the condition of high concentration of niacin, the activity of CAT,GSH-PX was significantly increased, T-AOC was enhanced, the concentration of MDA was decreased (P0.01), and the concentration of T-SOD was not affected (P0.05). (3) compared with the low concentrate group. The concentrations of proinflammatory factor IL-1 尾, IL-6,TNF- 偽 and anti inflammatory factor IL-4,IL-10 in sheep serum of high concentrate group were significantly higher than those of high concentrate group (P0.01), and IL-1 尾, IL-6, in serum of high concentrate group were significantly higher than those of high concentrate group (P0.01). The concentrations of TNF- 偽 and anti inflammatory factor IL-4,IL-10 were significantly lower than those of high concentrate group (P0.01). Compared with the low concentrate group, the rumen papilla length, width and cuticle thickness in the high concentrate group were significantly decreased (P0.01), and the apoptosis index was significantly increased (P0.01). The length, width and cuticle of rumen nipple in high concentrate niacin group were higher than those in high concentrate group (P0.01 or P0.05), and the apoptosis index (P0.01). (4) was significantly lower than that in low concentrate group. The rumen epithelium Fas,Bcl-2,Bax,P53 was up-regulated and the mRNA expression of Caspase-3,Caspase-8,Caspase-9 was significantly increased in high concentrate group (P0.01), and niacin significantly down-regulated Fas,Bcl-2,Bax, in high concentrate group (P0.01). P53 and Caspase-3,Caspase-8,Caspase-9 expression (P0.01). In conclusion, high concentrate stress increased the concentration of VFA and lactic acid in rumen of sheep, decreased the pH value, decreased the antioxidant ability of epithelial cells, up-regulated the expression of apoptosis-related gene P53, Bcl-2, and increased the expression of Caspase-3,Caspase-8, in sheep rumen. The increased expression of Caspase-9 resulted in apoptosis of rumen epithelial cells and impaired function of rumen epithelium. To some extent, niacin can relieve high concentrate stress, improve the antioxidant ability of rumen epithelial cells, inhibit the expression of apoptosis-related genes, inhibit the apoptosis of rumen epithelial cells, and protect the structure and function of rumen epithelium.
【學(xué)位授予單位】：江西農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：S826.5

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 劉政;王靜;曹平;王乃鳳;高愛(ài)琴;;不同水平的煙酸對(duì)AA肉仔雞血液生化指標(biāo)及抗氧化性能的影響[J];畜牧與獸醫(yī);2016年11期

2 林波;梁辛;韋升菊;李麗莉;梁賢威;李萍;鄒彩霞;;煙酸對(duì)水牛瘤胃發(fā)酵指標(biāo)及微生物區(qū)系的影響[J];動(dòng)物營(yíng)養(yǎng)學(xué)報(bào);2015年08期

3 鄭自通;庹勤慧;姚旭;陽(yáng)巍;鄭興;廖端芳;;煙酸聯(lián)合姜黃素對(duì)高脂大鼠血脂及肝臟炎癥因子表達(dá)的影響[J];中國(guó)藥事;2014年12期

4 劉玉潔;劉軍花;許婷婷;朱偉云;毛勝勇;;谷氨酰胺對(duì)飼喂高精料的奶山羊瘤胃上皮的保護(hù)效應(yīng)研究[J];草業(yè)學(xué)報(bào);2014年04期

5 歐陽(yáng)克蕙;張琪;魯友友;瞿明仁;熊小文;潘珂;;高精料飼糧中添加煙酸對(duì)體外瘤胃發(fā)酵培養(yǎng)液pH及發(fā)酵參數(shù)動(dòng)態(tài)變化的影響[J];動(dòng)物營(yíng)養(yǎng)學(xué)報(bào);2014年01期

6 楊淑青;高民;胡紅蓮;;亞急性瘤胃酸中毒產(chǎn)生機(jī)制及對(duì)瘤胃上皮屏障功能的影響[J];黑龍江畜牧獸醫(yī);2013年15期

7 翁秀秀;張養(yǎng)東;李發(fā)弟;周凌云;卜登攀;;不同類(lèi)型飼糧飼喂下奶牛瘤胃乳頭的光鏡和透射電鏡觀察[J];動(dòng)物營(yíng)養(yǎng)學(xué)報(bào);2013年09期

8 楊艷;瞿明仁;歐陽(yáng)克蕙;趙向輝;易中華;宋小珍;;煙酸對(duì)錦江黃牛瘤胃乳酸、揮發(fā)性脂肪酸濃度及相關(guān)酶活性的影響[J];動(dòng)物營(yíng)養(yǎng)學(xué)報(bào);2013年07期

9 歐陽(yáng)克蕙;魯友友;瞿明仁;黎觀紅;游金明;熊小文;;煙酸對(duì)高精料飼糧肥育肉牛生長(zhǎng)性能及血清生化指標(biāo)的影響[J];動(dòng)物營(yíng)養(yǎng)學(xué)報(bào);2012年09期

10 張波;劉宏斌;;煙酸抑制血管緊張素Ⅱ誘導(dǎo)的內(nèi)皮細(xì)胞凋亡[J];臨床和實(shí)驗(yàn)醫(yī)學(xué)雜志;2012年10期

相關(guān)會(huì)議論文 前2條

1 桂紅兵;沈贊明;;日糧精料水平對(duì)山羊瘤胃上皮生長(zhǎng)和細(xì)胞增殖及凋亡相關(guān)基因表達(dá)的影響[A];全國(guó)動(dòng)物生理生化第七屆全國(guó)代表大會(huì)暨第十三次學(xué)術(shù)交流會(huì)論文摘要匯編[C];2014年

2 陶詩(shī)煜;端木永前;董海波;倪迎冬;陳杰;沈向真;趙茹茜;;飼喂高精料日糧通過(guò)增加上皮細(xì)胞凋亡和炎癥反應(yīng)引發(fā)羊腸黏膜損傷[A];全國(guó)動(dòng)物生理生化第七屆全國(guó)代表大會(huì)暨第十三次學(xué)術(shù)交流會(huì)論文摘要匯編[C];2014年

相關(guān)博士學(xué)位論文 前2條

1 李婷;PSAP誘導(dǎo)獨(dú)特的不依賴(lài)于Bcl-2家族蛋白的線粒體凋亡途徑[D];吉林大學(xué);2012年

2 胡紅蓮;奶山羊亞急性瘤胃酸中毒營(yíng)養(yǎng)生理機(jī)制的研究[D];內(nèi)蒙古農(nóng)業(yè)大學(xué);2008年

相關(guān)碩士學(xué)位論文 前10條

1 張貝;日糧營(yíng)養(yǎng)水平對(duì)山羊瘤胃上皮細(xì)胞凋亡和自噬的影響[D];南京農(nóng)業(yè)大學(xué);2014年

2 張琪;煙酸及其拮抗物對(duì)瘤胃發(fā)酵參數(shù)及乳酸代謝的影響[D];江西農(nóng)業(yè)大學(xué);2014年

3 鄔宇航;亞急性瘤胃酸中毒對(duì)瘤胃、瓣胃上皮細(xì)胞增殖與凋亡的影響[D];內(nèi)蒙古農(nóng)業(yè)大學(xué);2013年

4 王靜;煙酸對(duì)肉仔雞生產(chǎn)性能、血液生化指標(biāo)及抗氧化性能的影響[D];內(nèi)蒙古農(nóng)業(yè)大學(xué);2013年

5 阿里婭古麗·依布熱依木;添喂煙酰胺和延胡索酸二鈉對(duì)綿羊消化代謝的影響[D];新疆農(nóng)業(yè)大學(xué);2013年

6 魯友友;煙酸對(duì)高精料日糧條件下肉牛生產(chǎn)性能和瘤胃體外發(fā)酵的影響[D];江西農(nóng)業(yè)大學(xué);2013年

7 屯妮薩·麥提賽伊迪;不同精粗比日糧、粗料型日糧添喂煙酰胺對(duì)綿羊瘤胃微生物代謝的影響[D];新疆農(nóng)業(yè)大學(xué);2012年

8 蔣亞軍;煙酸、核黃素對(duì)奶牛生產(chǎn)性能、抗氧化能力和免疫力的影響[D];石河子大學(xué);2011年

9 趙培廳;日糧不同NFC/NDF比對(duì)奶山羊瘤胃發(fā)酵功能和微生物區(qū)系變化的影響[D];內(nèi)蒙古農(nóng)業(yè)大學(xué);2011年

10 黃智南;日糧營(yíng)養(yǎng)對(duì)前胃上皮生長(zhǎng)和組織形態(tài)的影響[D];南京農(nóng)業(yè)大學(xué);2010年

，

本文編號(hào)：2373728