發(fā)布時間：2018-11-24 14:15

【摘要】：為研究呼吸道大腸桿菌對肉仔雞肝臟抗氧化能力的影響,選取24只初始體質(zhì)量相近、健康狀況良好的固始肉仔雞,隨機分為2組,對照組肉仔雞氣管滴注1 m L無菌培養(yǎng)液,大腸桿菌組肉仔雞氣管滴注1 m L大腸桿菌培養(yǎng)液(1×10~9cfu/m L)。滴注后2 h采集血液和肝臟組織,測定血清中細菌脂多糖(LPS)、谷丙轉(zhuǎn)氨酶(ALT)和谷草轉(zhuǎn)氨酶(AST)含量,以及肝臟組織中丙二醛(MDA)含量、谷胱甘肽過氧化物酶(GSH-PX)和超氧化物歧化酶(SOD)活性。結(jié)果表明,氣管滴注大腸桿菌后2 h,大腸桿菌組肉仔雞血清中LPS含量為33.86 U/mg,較對照組升高了26.15%;AST和ALT含量較對照組分別增加了50.89%(P0.05)和298.77%(P0.01);大腸桿菌組肉仔雞肝臟組織中SOD活性和GSH-PX活性分別為186.60 U/mg和13.20 U/mg,較對照組分別降低了43.83%(P0.01)和31.18%(P0.05);MDA含量增加了51.72%(P0.05)。綜上,氣管滴注大腸桿菌后2 h,大腸桿菌大量繁殖,釋放出的LPS通過血液循環(huán)進入肝臟,肝臟中抗氧化酶活性降低,引起脂質(zhì)過氧化物堆積,破壞肝臟細胞,導(dǎo)致血清中轉(zhuǎn)氨酶水平升高。
[Abstract]:In order to study the effect of respiratory Escherichia coli on liver antioxidation ability of broilers, 24 broilers with similar initial body mass and good health were randomly divided into 2 groups. The control group was treated with 1 mL aseptic culture solution. 1 mL Escherichia coli culture medium (1 脳 10~9cfu/m L).) in Escherichia coli group Blood and liver tissues were collected at 2 h after infusion. Serum lipopolysaccharide (LPS),) glutamate-alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, and malondialdehyde (MDA) content in liver tissue were determined. Glutathione peroxidase (GSH-PX) and superoxide dismutase (SOD) activities. The results showed that the content of LPS in serum of Escherichia coli group was 33.86 U / mg at 2 h after trachea drip, which was 26.15% higher than that of control group. The contents of AST and ALT increased by 50.89% (P0.05) and 298.77% (P0.01) respectively. The activity of SOD and GSH-PX in the liver tissue of Escherichia coli group were 186.60 U/mg and 13.20 U / mg, respectively, which decreased by 43.83% (P0.01) and 31.18% (P0.05) compared with the control group. The content of MDA increased by 51.72% (P0.05). In conclusion, 2 hours after trachea drip of Escherichia coli, Escherichia coli propagated in large numbers, the released LPS entered the liver through blood circulation, and the activity of antioxidant enzymes in the liver decreased, which caused the accumulation of lipid peroxides and destroyed the liver cells. Results in elevated serum levels of transaminase.
【作者單位】： 河南工業(yè)大學(xué)生物工程學(xué)院;
【基金】：河南省教育廳自然科學(xué)項目(15A230008) 河南省科技廳自然科學(xué)項目(142300410012) 河南工業(yè)大學(xué)高層次人才基金項目(2013BS027)
【分類號】：S858.31

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