【摘要】：[目的]研究p-STAT3與SOCS3在肉雞TD發(fā)生過程中的時(shí)序性表達(dá),初步探討JAK-STAT信號通路與肉雞TD的相關(guān)性。[方法]利用實(shí)驗(yàn)室建立的肉雞TD模型,采用Real-time qPCR、免疫組化技術(shù)研究TD發(fā)生過程中p-STAT3與SOCS3在轉(zhuǎn)錄水平和翻譯水平的時(shí)序性表達(dá)差異。[結(jié)果]與對照組相比,飼喂福美雙組肉雞生長板STAT3基因在第1天表達(dá)變化不明顯,第2、4、6天表達(dá)增加,差異極顯著(P0.01)。SOCS3基因在第1、2、6天表達(dá)增加,第4天表達(dá)降低,差異極顯著(P0.01)。p-STAT3蛋白在生長板增值區(qū)、前肥大區(qū)與肥大區(qū)均表達(dá)。與對照組相比,飼喂福美雙組增殖區(qū)p-STAT3蛋白在第1、2、4天表達(dá)均上調(diào),第1、2天差異極顯著(P0.01),第4天差異顯著(P0.05),第6天表達(dá)下調(diào)且差異顯著(P0.05)；在前肥大區(qū)與肥大區(qū),第1、2、4天均上調(diào)表達(dá),第6天表達(dá)量下調(diào),差異極顯著(P0.01)。SOCS3蛋白在生長板各區(qū)也均有表達(dá),與對照組相比,飼喂福美雙組增殖區(qū)SOCS3在第1、4、6天表達(dá)上調(diào),第2天表達(dá)下調(diào),其中第2、4天差異極顯著(P0.01)：在前肥大區(qū),第1、4、6天表達(dá)上調(diào),第2天表達(dá)下調(diào),其中第1、4天差異極顯著(P0.01),第2天差異顯著(P0.05);SOCS3在肥大區(qū)第1、2、4、6天均表達(dá)上調(diào),其中第1、2、4天差異極顯著(P0.01)。[結(jié)論]在轉(zhuǎn)錄水平STAI3與SOCS3基因表達(dá)均呈先上升后下降的趨勢,差異表達(dá)結(jié)果與基因芯片結(jié)果相吻合。p-STAT3和SOCS3蛋白在生長板增殖區(qū)、前肥大區(qū)與肥大區(qū)均有表達(dá)。上述結(jié)果提示,福美雙可能通過JAK-STAT信號通路調(diào)節(jié)軟骨細(xì)胞的增殖、分化及凋亡。
[Abstract]:[objective] to study the temporal expression of p-STAT3 and SOCS3 in broiler TD, and to explore the correlation between JAK-STAT signaling pathway and TD in broilers. [methods] the Real-time qPCR, immunohistochemical technique was used to study the temporal expression differences between p-STAT3 and SOCS3 at transcription level and translation level in the pathogenesis of TD by using the TD model of broilers established in laboratory. [results] compared with the control group, the expression of STAT3 gene in the growth plate of the control group was not significantly changed on the 1st day, but increased on the 2nd day (P0.01). The expression of SOCS3 gene increased on the 1st day (P0.01). On the 4th day, the expression of p-STAT3 protein decreased significantly (P0.01). The expression of p-STAT3 protein was found in the value-added region, prehypertrophic area and hypertrophic region of the growth plate. Compared with the control group, the expression of p-STAT3 protein in proliferative zone was up-regulated on the 1st day (P0.01) and the 4th day (P0.05) in both groups, and the difference was significant on the 1st day (P0.01) and the 4th day (P0.05). On the 6th day, the expression was down-regulated and the difference was significant (P0.05). In the prehypertrophic and hypertrophic regions, the expression of SOCS3 was up-regulated on the 4th day and down-regulated on the 6th day (P0.01). The expression of SOCS3 protein was also found in the growth plate. Compared with the control group, the expression of SOCS3 in the proliferative region of the two groups was significantly higher than that in the control group (P0.01). The expression was up-regulated on the 6th day and down-regulated on the second day. There was a significant difference on the 2nd day (P0.01): in the premast area, the expression of the 1st motif was up-regulated on the 6th day, and the expression was down-regulated on the second day, and the difference was very significant on the 1st day (P0.01). On the second day, the difference was significant (P0.05). The expression of SOCS3 was up-regulated on the 6th day (P0.01). [conclusion] at the transcriptional level, the expression of STAI3 and SOCS3 genes increased first and then decreased, and the differential expression results were consistent with the results of gene microarray. P-STAT3 and SOCS3 proteins were expressed in proliferative, prehypertrophic and hypertrophic regions of the growth plate. These results suggest that Fumex may regulate the proliferation, differentiation and apoptosis of chondrocytes through JAK-STAT signaling pathway.
【學(xué)位授予單位】：山西農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：Q78;S831

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 涂廣運(yùn);白世平;張克英;彭西;丁雪梅;曾秋鳳;;磷源對福美雙誘導(dǎo)的肉雞脛骨軟骨發(fā)育不良及骨骼鈣、磷代謝的影響[J];動(dòng)物營養(yǎng)學(xué)報(bào);2012年09期

2 宋舟;張立艷;董海兵;白換力;陳偉;吳帥成;伊鵬霏;付本懂;申海清;韋旭斌;;JAK-STAT信號通路研究進(jìn)展[J];中國畜牧獸醫(yī);2012年06期

3 韓春來,王麗明,鄭明學(xué),史喜菊;影響肉雞脛骨軟骨發(fā)育不良的營養(yǎng)因素[J];動(dòng)物科學(xué)與動(dòng)物醫(yī)學(xué);2001年03期

4 孫衛(wèi)東,王小龍;飲水高氯誘發(fā)肉雞脛骨軟骨發(fā)育不良的試驗(yàn)研究[J];畜牧獸醫(yī)學(xué)報(bào);2000年04期

5 李家奎;畢丁仁;潘思軼;張艷紅;郭會(huì)田;宋巍巍;;福美雙對肉仔雞肝功能和脛骨軟骨發(fā)育不良的影響[J];畜牧獸醫(yī)學(xué)報(bào);2006年09期

6 田文霞;李家奎;畢丁仁;張艷紅;覃平;;福美雙對肉雞脛骨軟骨發(fā)育不良發(fā)生的影響[J];畜牧與獸醫(yī);2006年08期

7 張建鵬;高奕;賈偉;郭碧凝;侯加法;;福美雙和銅對肉雞脛骨軟骨發(fā)育不良的影響[J];畜牧與獸醫(yī);2009年08期

8 田文霞;李家奎;畢丁仁;張艷紅;;肉雞脛骨軟骨發(fā)育不良分子機(jī)理的研究進(jìn)展[J];中國獸醫(yī)科學(xué);2006年07期

9 汪堯春,周毓平,咼于明,丁角立,朱玉琴;日糧硫、鎂水平對肉雞血液酸堿平衡和脛骨軟骨發(fā)育不良的影響[J];中國畜牧雜志;2000年01期

10 羅蘭,Steve R.Lee;不同來源的豆餅、日糧中氯和非植酸磷水平對肉用仔雞脛骨軟骨癥的影響[J];中國農(nóng)業(yè)科學(xué);1990年06期

相關(guān)博士學(xué)位論文 前1條

1 田文霞;福美雙誘發(fā)肉雞脛骨軟骨發(fā)育不良差異表達(dá)基因的研究[D];華中農(nóng)業(yè)大學(xué);2008年

相關(guān)碩士學(xué)位論文 前3條

1 李君偉;日糧電解質(zhì)平衡對生長肉兔生長性能、酸堿平衡、蛋白質(zhì)代謝和血液生化的影響[D];山東農(nóng)業(yè)大學(xué);2011年

2 卓麗玲;一氧化氮對肉雞脛骨軟骨發(fā)育不良作用機(jī)理的研究[D];四川農(nóng)業(yè)大學(xué);2005年

3 王瑞;ChM-I、VCAM-1基因在肉雞脛骨軟骨發(fā)育不良差異表達(dá)的時(shí)序性研究[D];華中農(nóng)業(yè)大學(xué);2009年

，

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