日糧蛋白水平對(duì)仔豬肝臟氨基酸代謝轉(zhuǎn)化的影響研究
發(fā)布時(shí)間：2018-11-18 18:41
【摘要】：本論文旨在研究日糧蛋白水平對(duì)仔豬肝臟氨基酸代謝轉(zhuǎn)化的影響規(guī)律及機(jī)制,包括以下四個(gè)研究內(nèi)容：研究內(nèi)容一：日糧蛋白水平對(duì)仔豬肝門靜脈氨基酸濃度及門靜脈凈吸收的影響。選擇18頭體重為20±1.0 kg的杜×長×大三元雜交豬,分別安裝門靜脈、頸動(dòng)脈、腸系膜靜脈和肝靜脈血插管,按單因子完全隨機(jī)設(shè)計(jì)分配到粗蛋白(CP)為20%、17%、14%的三組日糧中,每組6個(gè)重復(fù)。動(dòng)物自由飲水,每日于0800、1600、2400 h進(jìn)行飼喂。待動(dòng)物手術(shù)恢復(fù)后分別于0730、0930、1130、1430 h采集肝靜脈、門靜脈和頸動(dòng)脈血液樣品。結(jié)果表明,除谷氨酸(Glu)外,門靜脈其他氨基酸的濃度均高于肝靜脈(P0.05)；仔豬門靜脈、肝靜脈血漿總氨基酸(TAA)和非必需氨基酸(NEAA)濃度以及肝靜脈血漿必需氨基酸(EAA)濃度隨日糧蛋白水平的降低而顯著降低(P0.05),但是門靜脈血漿EAA濃度不受日糧蛋白水平的影響(P0.05)；20%、17%、14%蛋白組門靜脈血漿EAA濃度與NEAA濃度的比值分別為1.21、1.23、1.24,明顯高于肝靜脈中的相應(yīng)比值(P0.05)。降低日糧蛋白水平顯著降低門靜脈TAA、NEAA的凈吸收量(P0.05),但是對(duì)門靜脈EAA的凈吸收量無顯著影響(P0.05)；20%、17%、14%蛋白質(zhì)組門靜脈EAA凈吸收與NEAA凈吸收的比值分別為1.70、2.08和2.06。氨基酸經(jīng)過肝臟后TAA的絕對(duì)變化量不受日糧蛋白水平的影響(P0.05), NEAA的絕對(duì)變化量隨日糧蛋白水平的降低而顯著降低(P0.05),而EAA的絕對(duì)變化量隨日糧蛋白水平的降低而顯著升高(P0.05)；在肝臟中EAA絕對(duì)變化量與NEAA絕對(duì)變化量的比值隨日糧蛋白水平的降低而升高(P0.05)；經(jīng)過肝臟代謝后,Glu流通量有顯著增加。研究內(nèi)容二：日糧蛋白水平對(duì)仔豬物質(zhì)代謝的影響,試驗(yàn)設(shè)計(jì)同試驗(yàn)一。結(jié)果表明,隨著日糧蛋白水平的增加,門靜脈與肝靜脈血漿葡萄糖(GLU)和血尿素氮(BUN)濃度顯著上升(P0.05),但是日糧蛋白水平對(duì)頸動(dòng)脈GLU和BUN濃度無顯著影響(P0.05)。日糧蛋白水平對(duì)仔豬門靜脈、肝靜脈及頸動(dòng)脈總蛋白濃度無顯著影響(P0.05)。研究內(nèi)容三：日糧蛋白水平對(duì)仔豬肝臟氨基酸代謝轉(zhuǎn)化酶的影響。選擇54頭28日齡的杜×長×大三元雜交斷奶仔豬,采用單因子完全隨機(jī)設(shè)計(jì)分配到CP為20%、17%、14%的三組日糧中。試驗(yàn)預(yù)飼期為7 d,正試期為45 d。在正試期的第10 d、第25 d及第45 d,分別從每組挑選6頭仔豬進(jìn)行屠宰,采集肝臟樣品。結(jié)果表明,仔豬肝臟谷丙轉(zhuǎn)氨酶、谷草轉(zhuǎn)氨酶及谷氨酰胺合成酶的活力隨日糧蛋白水平的增加而顯著上升(P0.05),日糧蛋白水平對(duì)仔豬肝臟谷氨酸脫氫酶活力無顯著影響(P0.05)。研究內(nèi)容四,日糧蛋白水平對(duì)仔豬肝臟氨基酸轉(zhuǎn)運(yùn)載體表達(dá)的影響。試驗(yàn)設(shè)計(jì)同研究內(nèi)容三。結(jié)果表明,氨基酸轉(zhuǎn)運(yùn)載體SLC6A15、SLC6A20、SLC36A1及SLC38A2 mRNA表達(dá)量隨蛋白水平的增加而升高。小結(jié)：本研究表明,除了適當(dāng)?shù)慕档腿占Z蛋白水平及補(bǔ)充EAA外,還需補(bǔ)充特定的NEAA在日糧中的比例。這不僅可以降低氮的排放,維持氨基酸代謝轉(zhuǎn)化酶活力和氨基酸轉(zhuǎn)運(yùn)載體的表達(dá),還可以提高仔豬肝臟氨基酸代謝轉(zhuǎn)化效率。
[Abstract]:The purpose of this study is to study the effect of daily grain protein level on the transformation of liver amino acid metabolism in piglets, including the following four studies: the effect of the level of daily grain protein on the amino acid concentration of the portal vein and the net absorption of the portal vein in the piglets. The portal vein, the carotid artery, the mesenteric vein and the hepatic venous blood tube were respectively installed at 18 head weight of 20 to 1. 0kg. The total random design of the single factor was 20%, 17% and 14% of the three groups of daily food, and 6 repeats in each group. The animals were fed with free drinking water at 0800, 1600 and 2400 hours a day. The hepatic vein, portal vein and carotid blood samples were collected at 0730, 0930, 1130, and 1430h after the recovery of the animal. The results showed that, in addition to glutamic acid (Glu), the concentration of other amino acids in the portal vein was higher than that of the hepatic vein (P0.05); the portal vein of the piglets, The concentration of total amino acid (TAA) and non-essential amino acid (NEAA) in the hepatic vein and the concentration of essential amino acid (EAA) in the plasma of the hepatic vein decreased significantly with the decrease of the daily food protein level (P0.05), but the plasma EAA concentration in the portal vein was not affected by the protein level of the day (P0.05); 20%, The ratio of the concentration of EAA to the concentration of NEAA in the portal vein of the 14% protein group was 1.21, 1.23, and 1.24, respectively, which was higher than that in the hepatic vein (P0.05). The net absorption of TAA and NEAA in the portal vein was significantly lower than that of the portal vein (P0.05), but the net absorption of the portal vein (EAA) was not significantly affected (P0.05). The ratio of the net absorption of the portal vein (EAA) and the net absorption of the NEAA in the portal vein (EAA) of the portal vein (EAA) was 1.70, 2.08 and 2.06, respectively. The absolute change of TAA in the liver of the amino acid was not affected by the dietary protein level (P0.05). The absolute change of the NEA decreased significantly with the decrease of the daily food protein level (P0.05), while the absolute change of the EAA was significantly increased with the decrease of the daily food protein level (P0.05). The ratio of the absolute change of the EAA and the absolute change of the NEA in the liver increased with the decrease of the daily food protein level (P0.05); after the liver metabolism, the turnover of Glu increased significantly. Study content 2: The effect of daily grain protein level on the metabolism of the pig's substance and the design of the test were the same as that of the experiment. The results showed that the plasma glucose (GLU) and blood urea nitrogen (BUN) concentration in the portal vein and the hepatic vein were significantly increased with the increase of daily food protein level (P0.05), but the level of daily grain protein had no significant effect on the concentration of GLU and BUN in the carotid artery (P0.05). The level of daily grain protein had no significant effect on the total protein concentration of portal vein, hepatic vein and carotid artery (P0.05). The effect of dietary protein on the metabolism of amino acid in the liver of piglets was studied. A total of 20%, 17% and 14% of the three-day diets with a single factor of 20%, 17% and 14% were randomly assigned to the weaned piglets at the age of 54 to 28 days. The pre-feeding period of the trial was 7 days, and the positive test period was 45 days. 6 piglets were selected from each group for slaughter and the liver samples were collected during the 10th day, the 25th day and the 45th day of the positive test period. The results showed that the activity of the glutamic-pyruvic transaminase, the aspartate aminotransferase and the amino-aminopeptidase in the liver of the piglets increased significantly with the increase of the daily grain protein level (P0.05), and the dietary protein level had no significant effect on the activity of the liver glutamate dehydrogenase of the piglets (P0.05). The effect of dietary protein on the expression of amino acid transporter in piglets was studied. The test design is the same as the study content. The results showed that the expression of SLC6A15, SLC6A20, SLC36A1 and SLC38A2 mRNA increased with the increase of protein level. Summary: This study shows that, in addition to the proper reduction of dietary protein levels and the addition of EAA, the proportion of specific NEAA in daily food needs to be supplemented. The invention not only can reduce the emission of nitrogen, maintain the activity of the amino acid metabolism invertase and the expression of the amino acid transport vector, but also improve the conversion efficiency of the amino acid metabolism of the liver of the piglets.
【學(xué)位授予單位】：西南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S828.5
本文編號(hào)：2340834
[Abstract]:The purpose of this study is to study the effect of daily grain protein level on the transformation of liver amino acid metabolism in piglets, including the following four studies: the effect of the level of daily grain protein on the amino acid concentration of the portal vein and the net absorption of the portal vein in the piglets. The portal vein, the carotid artery, the mesenteric vein and the hepatic venous blood tube were respectively installed at 18 head weight of 20 to 1. 0kg. The total random design of the single factor was 20%, 17% and 14% of the three groups of daily food, and 6 repeats in each group. The animals were fed with free drinking water at 0800, 1600 and 2400 hours a day. The hepatic vein, portal vein and carotid blood samples were collected at 0730, 0930, 1130, and 1430h after the recovery of the animal. The results showed that, in addition to glutamic acid (Glu), the concentration of other amino acids in the portal vein was higher than that of the hepatic vein (P0.05); the portal vein of the piglets, The concentration of total amino acid (TAA) and non-essential amino acid (NEAA) in the hepatic vein and the concentration of essential amino acid (EAA) in the plasma of the hepatic vein decreased significantly with the decrease of the daily food protein level (P0.05), but the plasma EAA concentration in the portal vein was not affected by the protein level of the day (P0.05); 20%, The ratio of the concentration of EAA to the concentration of NEAA in the portal vein of the 14% protein group was 1.21, 1.23, and 1.24, respectively, which was higher than that in the hepatic vein (P0.05). The net absorption of TAA and NEAA in the portal vein was significantly lower than that of the portal vein (P0.05), but the net absorption of the portal vein (EAA) was not significantly affected (P0.05). The ratio of the net absorption of the portal vein (EAA) and the net absorption of the NEAA in the portal vein (EAA) of the portal vein (EAA) was 1.70, 2.08 and 2.06, respectively. The absolute change of TAA in the liver of the amino acid was not affected by the dietary protein level (P0.05). The absolute change of the NEA decreased significantly with the decrease of the daily food protein level (P0.05), while the absolute change of the EAA was significantly increased with the decrease of the daily food protein level (P0.05). The ratio of the absolute change of the EAA and the absolute change of the NEA in the liver increased with the decrease of the daily food protein level (P0.05); after the liver metabolism, the turnover of Glu increased significantly. Study content 2: The effect of daily grain protein level on the metabolism of the pig's substance and the design of the test were the same as that of the experiment. The results showed that the plasma glucose (GLU) and blood urea nitrogen (BUN) concentration in the portal vein and the hepatic vein were significantly increased with the increase of daily food protein level (P0.05), but the level of daily grain protein had no significant effect on the concentration of GLU and BUN in the carotid artery (P0.05). The level of daily grain protein had no significant effect on the total protein concentration of portal vein, hepatic vein and carotid artery (P0.05). The effect of dietary protein on the metabolism of amino acid in the liver of piglets was studied. A total of 20%, 17% and 14% of the three-day diets with a single factor of 20%, 17% and 14% were randomly assigned to the weaned piglets at the age of 54 to 28 days. The pre-feeding period of the trial was 7 days, and the positive test period was 45 days. 6 piglets were selected from each group for slaughter and the liver samples were collected during the 10th day, the 25th day and the 45th day of the positive test period. The results showed that the activity of the glutamic-pyruvic transaminase, the aspartate aminotransferase and the amino-aminopeptidase in the liver of the piglets increased significantly with the increase of the daily grain protein level (P0.05), and the dietary protein level had no significant effect on the activity of the liver glutamate dehydrogenase of the piglets (P0.05). The effect of dietary protein on the expression of amino acid transporter in piglets was studied. The test design is the same as the study content. The results showed that the expression of SLC6A15, SLC6A20, SLC36A1 and SLC38A2 mRNA increased with the increase of protein level. Summary: This study shows that, in addition to the proper reduction of dietary protein levels and the addition of EAA, the proportion of specific NEAA in daily food needs to be supplemented. The invention not only can reduce the emission of nitrogen, maintain the activity of the amino acid metabolism invertase and the expression of the amino acid transport vector, but also improve the conversion efficiency of the amino acid metabolism of the liver of the piglets.
【學(xué)位授予單位】：西南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S828.5
【引證文獻(xiàn)】
相關(guān)期刊論文 前2條
1 楊靜;許慶慶;李貌;陳澄;黃飛若;吳趙亮;孫志洪;;飼糧粗蛋白質(zhì)水平對(duì)仔豬門靜脈回流組織氮代謝的影響[J];中國畜牧雜志;2016年21期
2 孫志洪;李貌;許慶慶;印遇龍;朱偉云;江青艷;黃飛若;;豬氨基酸代謝節(jié)儉機(jī)制新假說[J];動(dòng)物營養(yǎng)學(xué)報(bào);2016年11期
相關(guān)碩士學(xué)位論文 前2條
1 楊靜;甘氨酸和丙氨酸在豬肝臟中的代謝去向研究[D];西南大學(xué);2016年
2 吳趙亮;不同生長階段豬門靜脈排流組織氨基酸代謝規(guī)律研究[D];西南大學(xué);2016年
，本文編號(hào)：2340834
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