【摘要】：內蒙古絨山羊具有優(yōu)良的產絨性狀,是寶貴的種質遺傳資源,因此研究絨山羊的絨毛發(fā)育機理,探尋與絨毛生長相關的重要候選基因就顯得尤為重要。本試驗采用高通量測序技術,選取3只內蒙古白絨山羊絨毛發(fā)育不同時期(生長期11月份、退行期1月份、休止期3月份)體側部皮膚初級毛囊和次級毛囊各3個RNA樣本,等量混合后進行轉錄組測序,并對結果進行生物信息學分析及qRT-PCR驗證。試驗結果顯示：1、試驗分別對3只不同生長時期絨山羊的初級、次級毛囊進行了組間和組內的差異基因篩選,共得到9組差異基因,并對差異基因進行了GO和Pathway分析。GO分析顯示：顯著富集的條目大多與組織細胞外基質、核糖體結構組成、細胞平移伸長、胞質小核糖體、肌動蛋白及維生素K的代謝過程有關。Pathway分析顯示：差異基因主要在核糖體代謝、氨基酸代謝、維生素代謝、硫代謝、新霉素生物合成等功能上影響顯著。2、試驗對絨山羊不同生長時期初級、次級毛囊組內進行差異基因篩選,發(fā)現(xiàn)這些基因都有8種連續(xù)的趨勢變化,分別是：down down down: up up down: down up down down up up: up down dowm; up down up:down down up: up up up；且在初、次級毛囊組內前三種趨勢都較為顯著。對這8中趨勢的差異基因進行分析研究,將對挖掘絨山羊毛囊(尤其是次級毛囊)周期性生長調控機理提供有力依據。3、試驗采用qRT-PCR技術對絨山羊毛囊發(fā)育的11月份及3月份的LGR5和SOSTDC1基因的轉錄組測序結果進行驗證。結果顯示,待測基因LGR5.SOSTDC1在絨山羊毛囊發(fā)育的11月份相對表達量顯著高于3月份。這與測序結果基本一致。說明測序結果真實有效。
[Abstract]:Inner Mongolia cashmere goats are valuable germplasm genetic resources, so it is very important to study the mechanism of cashmere development and to search for important candidate genes related to the growth of cashmere in Inner Mongolia Cashmere Goat (Inner Mongolia Cashmere Goat). High throughput sequencing technique was used to select 3 RNA samples of primary hair follicles and secondary hair follicles of three Inner Mongolia white cashmere goats at different stages (growth period November, retreat period January, rest month March). The results were analyzed by bioinformatics and qRT-PCR. The results showed that: 1, 9 groups of differential genes were obtained from the primary and secondary hair follicles of three cashmere goats at different growth stages. GO and Pathway analysis of differentially expressed genes were carried out. GO analysis showed that most of the items significantly enriched were associated with extracellular matrix, ribosomal structure, cell translocation and elongation, and cytoplasmic small ribosome. Pathway analysis showed that differential genes mainly affect ribosomal metabolism, amino acid metabolism, vitamin metabolism, sulfur metabolism, neomycin biosynthesis and other functions. Different genes were screened in the primary and secondary hair follicles of cashmere goats at different growth stages. It was found that all of these genes had eight continuous trend changes, respectively,: down down: up down: down up up: up down dowm;. Up down up: up up; At the beginning, the first three trends in the secondary hair follicle group were significant. The analysis of the differentially expressed genes of these eight trends will provide a powerful basis for digging out the mechanism of periodic growth regulation of cashmere goat hair follicles (especially secondary hair follicles). The transcriptome sequencing of LGR5 and SOSTDC1 genes in wool follicles of cashmere goats in November and March were verified by qRT-PCR technique. The results showed that the relative expression of LGR5.SOSTDC1 in wool follicles of cashmere goats in November was significantly higher than that in March. This is basically consistent with the sequencing results. The results show that the sequencing results are true and effective.
【學位授予單位】：內蒙古農業(yè)大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：S827

【參考文獻】

相關期刊論文 前10條

1 閆紅玲,梁大春,呂志,鮑斌,溫衛(wèi)東;阿拉善白絨山羊毛囊群體結構的觀察分析[J];中國草食動物;2000年05期

2 李金泉,尹俊,周歡敏;絨山羊絨毛生長調控機理研究進展[J];中國草食動物;2001年03期

3 王凌燕,王樹迎,尹遜河,張金花;沂蒙黑山羊與濟寧青山羊板皮組織結構的研究[J];中國草食動物;2004年06期

4 魏云霞;;絨山羊絨毛生長機理研究進展[J];中國草食動物;2008年03期

5 陳旭;齊鳳坤;康立功;李景富;;實時熒光定量PCR技術研究進展及其應用[J];東北農業(yè)大學學報;2010年08期

6 于洋,楊曉娟,黃莉,孫淑云,達文政;絨山羊皮膚毛囊結構和遺傳[J];甘肅畜牧獸醫(yī);2003年01期

7 趙艷紅;張燕軍;杜晨光;張文廣;李金泉;;內蒙古阿爾巴斯絨山羊毛囊結構特征分析[J];湖北農業(yè)科學;2009年02期

8 金銳;;實時熒光定量PCR應用技術綜述[J];科教文匯(中旬刊);2007年10期

9 吳明煜,郭曉紅,王萬賢,柯文山,楊毅,吳燕;生物芯片研究現(xiàn)狀及應用前景[J];科學技術與工程;2005年07期

10 宏偉;鄭云勝;倪利平;;內蒙古阿爾巴斯白絨山羊皮膚毛囊結構研究[J];畜牧與飼料科學;2008年01期

相關博士學位論文 前2條

1 吳江鴻;Hoxc13基因在絨山羊皮膚中的表達規(guī)律及體外功能分析[D];內蒙古農業(yè)大學;2011年

2 張燕軍;內蒙古絨山羊胎兒期皮膚毛囊形態(tài)發(fā)生和發(fā)育規(guī)律及部分Hox基因家族成員研究[D];內蒙古農業(yè)大學;2007年

相關碩士學位論文 前1條

1 常子麗;皮膚毛囊基因調控網絡的初建及其在絨山羊上的功能預測[D];內蒙古農業(yè)大學;2007年

，

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