發(fā)布時(shí)間：2018-11-02 15:57

【摘要】：豬天然抗性相關(guān)巨噬細(xì)胞蛋白基因1(Naturalresistance-associated Marophage Protein 1,Nramp1)作為影響抗病力的重要候選基因之一,在機(jī)體的免疫過程中發(fā)揮著重要的調(diào)控作用。為了在細(xì)胞水平上探討Nramp1基因與引起仔豬腹瀉的致病性大腸桿菌的關(guān)系及其在大腸桿菌感染過程中發(fā)揮的免疫調(diào)控作用,本研究利用F18ab、F18ac和K88ac等3種致病性大腸桿菌刺激體外培養(yǎng)的豬腸上皮細(xì)胞IPEC-J2,并利用實(shí)時(shí)熒光定量PCR檢測(cè)3種菌體刺激IPEC-J2后Nramp1基因的表達(dá)變化情況。結(jié)果表明:F18ab、F18ac和K88ac 3種大腸桿菌刺激IPEC-J2后,Nramp1基因的表達(dá)均發(fā)生極顯著(P0.01)上調(diào),差異倍數(shù)分別為10、8、11倍,且F18ab和K88ac 2種菌體刺激后Nramp1基因的表達(dá)上調(diào)程度均極顯著高于F18ac。本研究結(jié)果提示,Nramp1基因的表達(dá)與大腸桿菌的侵染有很大的相關(guān)性,其在大腸桿菌侵襲豬腸道中發(fā)揮了重要的免疫調(diào)控作用。本研究在細(xì)胞水平分析探討了Nramp1基因的表達(dá)與3種致病性大腸桿菌侵襲的關(guān)系,為Nramp1基因在大腸桿菌侵染機(jī)體的過程中發(fā)揮的免疫調(diào)控作用研究提供了參考依據(jù)。
[Abstract]:Porcine natural resistance-associated macrophage protein gene 1 (Naturalresistance-associated Marophage Protein 1 + Nramp1), as one of the most important candidate genes affecting disease resistance, plays an important regulatory role in the immune process of the body. In order to investigate the relationship between Nramp1 gene and pathogenic Escherichia coli causing diarrhea in piglets and its role in the immune regulation of Escherichia coli infection at the cellular level, F18ab was used in this study. Three pathogenic Escherichia coli, F18ac and K88ac, stimulated IPEC-J2, of cultured porcine intestinal epithelial cells in vitro, and real-time fluorescent quantitative PCR was used to detect the changes of Nramp1 gene expression in IPEC-J2 stimulated by three kinds of E. coli. The results showed that the expression of Nramp1 gene was significantly up-regulated (P0.01) after the stimulation of IPEC-J2 by F18aba F18ac and K88ac, and the difference was 10 ~ 8 ~ (11) times, respectively. The upregulation of Nramp1 gene expression by F18ab and K88ac was significantly higher than that of F18ac. The results suggest that the expression of Nramp1 gene is closely related to the infection of Escherichia coli, which plays an important role in the immune regulation of Escherichia coli invasion of pig intestine. In this study, the relationship between the expression of Nramp1 gene and the invasion of three pathogenic Escherichia coli was analyzed at the cell level, which provides a reference for the study of the immune regulation of Nramp1 gene in the process of E. coli infection.
【作者單位】： 揚(yáng)州大學(xué)動(dòng)物科學(xué)與技術(shù)學(xué)院江蘇省動(dòng)物遺傳繁育與分子設(shè)計(jì)重點(diǎn)實(shí)驗(yàn)室;江蘇省種豬繁育和健康養(yǎng)殖工程技術(shù)研究中心;
【基金】：江蘇省科技支撐計(jì)劃(BE2015329) 江蘇省農(nóng)業(yè)三新工程項(xiàng)目(SXGC[2015]326、SXGC[2016]097)
【分類號(hào)】：S858.28

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