【摘要】：本研究旨在研究妊娠后期營養(yǎng)限制對母羊胃腸道葡萄糖轉(zhuǎn)運(yùn)載體相關(guān)基因表達(dá)的影響。選取20只同期受孕的湘東黑山羊,隨機(jī)分為2組,即對照組(自由采食)和限飼組(40%采食量限制),每組10只。預(yù)試期15 d(妊娠81~95 d),正試期39 d(妊娠96~135 d)。正試期結(jié)束后,屠宰并采取瘤胃、十二指腸、空腸、回腸以及盲腸的黏膜樣品,利用實(shí)時(shí)定量PCR技術(shù),檢測Na+-葡萄糖共轉(zhuǎn)運(yùn)載體1(SGLT1)、Na+-葡萄糖共轉(zhuǎn)運(yùn)載體3(SGLT3)、易化葡萄糖轉(zhuǎn)運(yùn)載體2(GLUT2)和易化葡萄糖轉(zhuǎn)運(yùn)載體5(GLUT5)基因表達(dá)量。結(jié)果表明:限飼組與對照組相比,SGLT1基因表達(dá)量在瘤胃顯著降低(P0.05),在空腸和回腸中有降低趨勢(0.05≤P0.10);GLUT5基因表達(dá)量在盲腸顯著降低(P0.05);而其他葡萄糖轉(zhuǎn)運(yùn)載體基因胃腸道表達(dá)量在限飼組和對照組差異均不顯著(P0.05)。由此可見,母羊妊娠后期營養(yǎng)限制對胃腸道中葡萄糖轉(zhuǎn)運(yùn)載體基因表達(dá)有不同程度的影響,進(jìn)而引起母羊機(jī)體內(nèi)葡萄糖轉(zhuǎn)運(yùn)的改變。
[Abstract]:The aim of this study was to investigate the effects of nutritional restriction on the expression of glucose transporter related genes in the gastrointestinal tract of ewe. Twenty Xiangdong black goats who were pregnant at the same time were randomly divided into two groups: the control group (free feed intake) and the restricted feeding group (40% feed intake restriction), with 10 goats in each group. Pretest period 15 days (pregnancy 81: 95 d),) 39 days (pregnancy 96135 d).) The mucosal samples of rumen, duodenum, jejunum, ileum and cecum were slaughtered at the end of the trial period. Na glucose co-transport vector 1 (SGLT3) was detected by real-time quantitative PCR. The gene expression of glucose transport vector 2 (GLUT2) and glucose transport vector 5 (GLUT5) was facilitated. The results showed that compared with the control group, the expression of SGLT1 gene decreased significantly in rumen (P0.05), decreased in jejunum and ileum (0.05 鈮，

本文編號：2304499