羊口瘡病程中Th17細(xì)胞相關(guān)細(xì)胞因子的變化
[Abstract]:Sheep contact with infectious pustular dermatitis, or Contagiouts Ecthyma,CE or ORF), is one of the most common diseases in sheep production in the world. It is widely used in Jilin, Ningxia, Yunnan, Henan and Hubei provinces in China. Xinjiang and Shaanxi provinces have reported that goats are more susceptible to infection than sheep, and the incidence of goat farms is usually 10% and 100%. Although the disease itself rarely leads to adult goat death, but to the newborn lamb serious harm, infection of mouth and lips caused by serious ulcers affect the feeding of lambs, and eventually lead to death; In addition, the disease also endangers the productive performance of adult sheep, causing mastitis and other diseases. The clinical features of sheep mouth ulcers include papules, blisters, pustules, skin rupture and finally scab, which is a typical inflammatory reaction. What kinds of cells and cytokines are involved in the process of this inflammation have not been reported yet. In this study, histopathological techniques, lymphocyte proliferation, real-time PCR and prokaryotic expression were used to study the characteristics of epithelial lesions in the course of sheep mouth ulcers. The healthy goat peripheral blood mononuclear lymphocytes (Peripheral blood mononuclear cells,PBMCs) were stimulated by the strong and weak strains of sheep mouth sore, and the proliferation of PBMCs and the level of cytokine mRNA were detected. At the same time, the levels of cytokine mRNA and the expression of IL-1 尾 and IL-6 protein were detected in sheep infected with aphtha. The results are as follows: 1. The main histological changes of skin injury caused by sheep mouth sore were vacuolar degeneration of epithelial cells and infiltration of inflammatory cells in subbasal connective tissue. With the development of the course of disease, the number of infiltrating cells increased, and the number of infiltrating cells was the most in pemphigus stage, and the number of infiltrating cells decreased gradually after entering the convalescent stage. Infiltrating cells mainly include lymphocytes, neutrophils, macrophages and other immune cells. The infiltration of inflammatory cells was mainly neutrophilic granulocyte in the early stage of the lesion, lymphocyte infiltration in blister and pemphigus, and lymphocyte and macrophage in convalescent stage. 2. Sheep mouth sore was strong and attenuated strains could significantly stimulate goat PBMCs proliferation. The effect of virulent strain was better than that of attenuated strain. The levels of IL-17 mRNA, IL-1 尾, IL-6 and IL-23 in PBMCs increased significantly, while TGF- 尾 mRNA decreased significantly. The level of cytokine mRNA produced by PBMCs in sheep mouth sore course showed that the levels of IL-1 尾, IL-6 and IL-23 mRNA in vesicular phase were significantly increased, IL-17 mRNA level in pemphigus phase was significantly increased, and IFN- 緯 mRNA level was also significantly increased. 3. Goat IL-1 尾 and IL-6 genes were cloned. The target fragments were 463 bp and 552 bp, prokaryotic expression fusion proteins, respectively. The size of IL-1 尾 and IL-6 fusion proteins were 37.4 kDa and 40.6 kDa, respectively. The polyclonal antibodies were prepared by immunizing rabbits. The titers of serum antibodies determined by Agar double diffusion were 1:16 and 1: 8, respectively. The results show that: 1. The pathological process of sheep mouth ulcer is essentially immune pathological reaction. The immune cells involved in this process are neutrophils at the early stage and lymphocytes at the later stage. The main cytokine that mediates the immune pathological reaction of sheep mouth sore is IL. According to the results of other cytokines measured in this study, we speculated that the IL 17 in sheep aphthous ulcer reaction originated from Th17 cells, in addition, Th1 cell subsets were also involved in the inflammatory reaction of sheep aphtha. 3. The cloning and expression of goat IL 1 尾, IL 6 protein and antiserum provide the preparation for further study of the immune pathological mechanism of sheep aphthous ulcer in our laboratory.
【學(xué)位授予單位】:西北農(nóng)林科技大學(xué)
【學(xué)位級(jí)別】:碩士
【學(xué)位授予年份】:2015
【分類號(hào)】:S858.27
【相似文獻(xiàn)】
相關(guān)期刊論文 前10條
1 劉玉芬,劉洪雨,張奎武;羊口瘡的診斷與治療[J];黑龍江畜牧獸醫(yī);2004年10期
2 王炳清;;山羊口瘡的診治體會(huì)[J];畜牧獸醫(yī)雜志;2008年04期
3 鮮思美;胡廷帥;熊朝麗;向智龍;陳秀華;;貴州省羊口瘡的調(diào)查研究[J];動(dòng)物醫(yī)學(xué)進(jìn)展;2012年11期
4 李前瑞;李杰;田婷婷;姚運(yùn)亮;許君艷;趙燕青;陳德坤;張淼濤;;羊口瘡病毒多重PCR檢測(cè)方法的建立[J];動(dòng)物醫(yī)學(xué)進(jìn)展;2013年06期
5 蔣建;;羊口瘡臨床病理與防治[J];當(dāng)代畜牧;2014年20期
6 羅燕雯,陳興芳,王玉智,馬愛榮,李平,高金梅;用犢牛睪丸細(xì)胞生產(chǎn)羊口瘡疫苗時(shí)應(yīng)注意的問題[J];中國獸藥雜志;2003年07期
7 張廷勝;;碘酊治療羊口瘡[J];中獸醫(yī)學(xué)雜志;2007年01期
8 任寶忠;夏會(huì)秋;王淑娟;;羊口瘡病的防治[J];畜牧與飼料科學(xué);2007年05期
9 顏新敏;張強(qiáng);;羊口瘡病毒的研究進(jìn)展[J];中國動(dòng)物檢疫;2008年11期
10 王樹春;周關(guān)發(fā);李志華;高林生;;中西醫(yī)結(jié)合治療羔羊口瘡[J];云南畜牧獸醫(yī);2008年02期
相關(guān)會(huì)議論文 前2條
1 何水林;陳杰;蔡玉書;姚龍濤;;羊口瘡病毒的分離與鑒定[A];第四屆中國畜牧獸醫(yī)青年科技工作者學(xué)術(shù)研討會(huì)論文集[C];2001年
2 劉遠(yuǎn);李文楊;林仕欣;高承芳;張曉佩;董曉寧;;福建省羊口瘡的流行病學(xué)調(diào)查與PCR診斷[A];中國畜牧獸醫(yī)學(xué)會(huì)養(yǎng)羊?qū)W分會(huì)2012年全國養(yǎng)羊生產(chǎn)與學(xué)術(shù)研討會(huì)議論文集[C];2012年
相關(guān)重要報(bào)紙文章 前3條
1 華坪縣畜牧獸醫(yī)站 楊永軍;不可小視羊口瘡病[N];云南科技報(bào);2013年
2 張國華;淺析山羊口瘡的綜合防治措施[N];中國畜牧獸醫(yī)報(bào);2006年
3 ;綜合防治山羊口瘡[N];中國畜牧獸醫(yī)報(bào);2007年
相關(guān)碩士學(xué)位論文 前10條
1 楊雯昱;羊口瘡病程中Th17細(xì)胞相關(guān)細(xì)胞因子的變化[D];西北農(nóng)林科技大學(xué);2015年
2 田婷婷;羊口瘡疫苗研制及其免疫效果評(píng)估[D];西北農(nóng)林科技大學(xué);2013年
3 張瑜;羊口瘡病毒楊凌株和瀘西株的分離鑒定及毒力致弱研究[D];西北農(nóng)林科技大學(xué);2014年
4 張立強(qiáng);羊口瘡病原分子流行病學(xué)調(diào)查及蜂膠佐劑滅活疫苗免疫效果研究[D];西北農(nóng)林科技大學(xué);2014年
5 高晶暉;羊口瘡病毒威海株和昆明株的分離鑒定及毒力致弱研究[D];西北農(nóng)林科技大學(xué);2014年
6 賈云曉;富平某奶山羊養(yǎng)殖場(chǎng)羊口瘡分子病原學(xué)調(diào)查及滅活疫苗免疫效果評(píng)估[D];西北農(nóng)林科技大學(xué);2014年
7 劉方;楊凌地區(qū)羊口瘡流行病學(xué)調(diào)查、病毒分離鑒定及相關(guān)基因的生物信息學(xué)分析[D];西北農(nóng)林科技大學(xué);2014年
8 李杰;羊口瘡抗體ELISA檢測(cè)方法的建立及應(yīng)用[D];西北農(nóng)林科技大學(xué);2013年
9 孔漢金;羊口瘡病毒感染宿主細(xì)胞的蛋白質(zhì)組變化及功能分析[D];華中農(nóng)業(yè)大學(xué);2014年
10 李浩;不同地區(qū)羊口瘡病毒三個(gè)主要結(jié)構(gòu)蛋白基因的遺傳進(jìn)化分析[D];內(nèi)蒙古農(nóng)業(yè)大學(xué);2015年
,本文編號(hào):2298245
本文鏈接:http://sikaile.net/yixuelunwen/dongwuyixue/2298245.html