【摘要】：駱駝具有耐鹽和耐干旱等顯著特點(diǎn)。研究發(fā)現(xiàn),駱駝耐鹽耐旱基因的啟動(dòng),可能需要鈣離子的參與。鈣調(diào)素通過(guò)與鈣離子的結(jié)合而激活,因此,可以通過(guò)鈣調(diào)素的表達(dá)來(lái)觀察鈣離子的變化。本實(shí)驗(yàn)通過(guò)給駱駝禁鹽、灌鹽、禁水和禁水后暴飲等處理,采取免疫熒光染色技術(shù),對(duì)不同處理后駱駝腎皮質(zhì)、腎髓質(zhì)、十二指腸、回腸、空腸和結(jié)腸組織中鈣調(diào)素的表達(dá)進(jìn)行了研究,以探討鈣離子與駱駝水鹽代謝相關(guān)基因表達(dá)的關(guān)系。其主要研究結(jié)果如下：1、克隆獲得了雙峰駝的鈣調(diào)素基因序列,并證明其能在Hela真核細(xì)胞內(nèi)表達(dá)。2、經(jīng)不同處理后,雙峰駝腎皮質(zhì)、腎髓質(zhì)、十二指腸、空腸、回腸、結(jié)腸組織中鈣調(diào)素均有表達(dá)。但在不同的處理組中,其表達(dá)的強(qiáng)度有所不同,即在灌鹽組與禁水組中較高,對(duì)照組與禁水后暴飲組次之,禁鹽組中較低。3、在不同處理組的不同組織中,鈣調(diào)素表達(dá)的位置分布也不同,即在腎皮質(zhì)中,鈣調(diào)素在腎小體足細(xì)胞與腎小管近曲小管中表達(dá)強(qiáng)度較高,腎小管遠(yuǎn)曲小管中較低；在腎髓質(zhì)中,鈣調(diào)素在腎小管的遠(yuǎn)曲小管中表達(dá)強(qiáng)度較高,而在集合小管中較低。在腸道組織中,鈣調(diào)素在十二指腸、空腸和回腸的絨毛上皮細(xì)胞中表達(dá)強(qiáng)度較高,而在結(jié)腸的腸腺細(xì)胞中表達(dá)強(qiáng)度較高。4、不同處理不同組織中,鈣調(diào)素表達(dá)的變化說(shuō)明了鈣離子的變化,間接說(shuō)明了鈣離子與駱駝水鹽代謝相關(guān)基因表達(dá)的可能關(guān)系。
[Abstract]:Camel has the characteristic of salt tolerance and drought tolerance. It has been found that the initiation of salt-tolerant drought-tolerant genes in camels may require the participation of calcium ions. Calmodulin is activated by binding with calcium ion, so the change of calcium ion can be observed by the expression of calmodulin. In this experiment, the camel renal cortex, medulla, duodenum and ileum were treated with immunofluorescence staining by salt, water and drinking. The expression of calmodulin in jejunum and colon was studied in order to explore the relationship between calcium ion and the expression of genes related to water salt metabolism in camels. The main results are as follows: 1. The calmodulin gene sequence of Bactrian camel was cloned and proved to be expressed in Hela eukaryotic cells. After different treatments, the renal cortex, medulla, duodenum, jejunum, ileum of bactrian camel were obtained. Calmodulin was expressed in colon tissue. However, the intensity of expression was different in different treatment groups, that is, higher in salt irrigation group and water deprivation group, second in control group and drinking after water ban group, and lower in salt ban group, and in different tissues of different treatment groups. The expression of calmodulin in renal cortex was higher in podocytes and proximal tubules, lower in distal tubules, and in renal medulla. Calmodulin expression was higher in the distal tubules of the renal tubules than in the collective tubules. In intestinal tissues, calmodulin expression was higher in chorionic epithelial cells of duodenum, jejunum and ileum, but higher in intestinal gland cells of colon. The change of calmodulin expression indicated the change of calcium ion and indirectly explained the possible relationship between calcium ion and the expression of genes related to water salt metabolism in camels.
【學(xué)位授予單位】：內(nèi)蒙古農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類(lèi)號(hào)】：S824

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