【摘要】：【目的】從豬新鮮糞便和腸道黏膜中篩選乳酸菌,為豬益生劑飼料添加劑提供理論依據(jù)和可靠菌種�！痉椒ā坎杉兾靼嗣钾i的糞便和腸道組織,分離純化乳酸菌,通過體外耐酸、耐膽汁試驗以及抑菌試驗和黏附試驗,篩選特性優(yōu)良的益生菌,用16SrRNA基因序列系統(tǒng)發(fā)育樹確定其分類地位。采用小鼠安全性檢驗試驗,檢測篩選菌株的安全性。【結(jié)果】篩選出一株耐受性和抑菌性良好,生長曲線穩(wěn)定且黏附性較好的乳酸菌,命名為FB027;另一株抑菌性和黏附性良好,生長曲線穩(wěn)定且耐酸、耐膽鹽較好的乳酸菌,命名為SE013。16SrRNA基因序列系統(tǒng)發(fā)育樹分析發(fā)現(xiàn),FB027為非解乳糖鏈球菌(Streptococcus alactolyticus),進化與同屬的血紅鏈球菌Streptococcus sanguinis(GenBank登錄號CP000387.1)同源性最高,SE013為腸系膜明串珠菌(Leuconostoc mesenteroides),進化與同屬的偽明串珠菌Leuconostoc pseudomesenteroides(GenBank登錄號AB572039.1)同源性最高。小鼠安全性檢驗試驗發(fā)現(xiàn),連續(xù)2周口服乳酸菌FB027和SE013,小鼠健康狀況良好,行為、生長、采食量和病理組織剖檢均無明顯異常。【結(jié)論】分離獲得了性能優(yōu)良的2株乳酸菌,其在豬飼料添加劑方面有潛在的應(yīng)用前景。
[Abstract]:[objective] to screen lactic acid bacteria from pig fresh feces and intestinal mucosa, and to provide theoretical basis and reliable bacteria for pig probiotics feed additive. [methods] feces and intestinal tissues of Shaanxi Bamei pig were collected, and lactic acid bacteria were isolated and purified. Probiotics with excellent characteristics were screened by acid tolerance, bile tolerance test, bacteriostatic test and adhesion test in vitro. The phylogenetic tree of 16SrRNA gene sequence was used to determine the taxonomic status of probiotics. The safety test of mice was used to detect the safety of the selected strains. [results] A lactic acid strain with good tolerance and bacteriostasis, stable growth curve and good adhesion was selected, which was named FB027; another strain with good bacteriostasis and adhesion. Lactic acid bacteria with stable growth curve and good bile salt resistance. Phylogenetic tree analysis named SE013.16SrRNA gene sequence showed that FB027 was the highest homology of (Streptococcus alactolyticus), evolution of non-lactose streptococcus and Streptococcus sanguinis (GenBank accession number CP000387.1 of the same genus, and SE013 was the pseudomembrane of (Leuconostoc mesenteroides), evolution and homologous genus of Streptococcus mesentericus. The homology of Leuconostoc pseudomesenteroides (GenBank accession number AB572039.1 was the highest. The safety test of mice showed that two strains of lactic acid bacteria, FB027 and SE013, had good health condition, no obvious abnormal behavior, growth, feed intake and pathological tissue. [conclusion] two strains of lactic acid bacteria with good performance were isolated. It has potential application prospect in pig feed additive.
【作者單位】： 西北農(nóng)林科技大學動物醫(yī)學院;西北農(nóng)林科技大學動物科技學院;
【基金】：國家自然科學基金項目(31201939)
【分類號】：S852.61

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