【摘要】：為分析河南省豬流行性腹瀉病毒(PEDV)分子和遺傳進(jìn)化特征,本研究對(duì)2014年1月~2015年12月收集自河南省不同地區(qū)的200份PEDV疑似病料進(jìn)行RT-PCR檢測(cè),并對(duì)15份陽性樣品進(jìn)行S和ORF3基因的克隆及序列分析,結(jié)果顯示15株河南流行株P(guān)EDV的S基因和ORF3基因的部分核苷酸和氨基酸序列與參考株相比均發(fā)生了變異。S和ORF3基因遺傳進(jìn)化樹顯示,PEDV均可分為兩個(gè)群,基于S基因的進(jìn)化樹顯示15株河南株獨(dú)立成群,與其它參考株親緣關(guān)系較遠(yuǎn);基于ORF3基因的進(jìn)化樹顯示,15株河南株與國內(nèi)分離株、美國株及韓國株均有較近的親緣關(guān)系。從S和ORF3基因的進(jìn)化關(guān)系中還顯示,15株河南株與經(jīng)典株CV777及國內(nèi)所使用的疫苗株,親緣關(guān)系較遠(yuǎn)。表明PEDV河南株有變異和進(jìn)化的趨勢(shì)。本研究為更好地控制本病在河南地區(qū)的發(fā)生奠定基礎(chǔ)。
[Abstract]:In order to analyze the molecular and genetic characteristics of porcine epidemic diarrhea virus (PEDV) in Henan Province, 200 suspected PEDV samples collected from different areas of Henan Province from January 2014 to December 2015 were detected by RT-PCR. The S and ORF3 genes were cloned and sequenced from 15 positive samples. The results showed that the nucleotide and amino acid sequences of the S gene and the ORF3 gene of 15 Henan epidemic strains were mutated compared with the reference strains. The S and ORF3 gene genetic evolution tree showed that PEDV could be divided into two groups. The phylogenetic tree based on S gene showed that 15 Henan strains were independent in groups and had a close relationship with other reference strains, while the evolutionary tree based on ORF3 gene showed that 15 Henan strains were closely related to domestic isolates, American strains and Korean strains. From the evolutionary relationship of S and ORF3 genes, 15 Henan strains were closely related to the classical strain CV777 and the vaccine strains used in China. The results indicated that PEDV Henan strain had the tendency of variation and evolution. This study lays a foundation for better control of the disease in Henan province.
【作者單位】： 吉林大學(xué)動(dòng)物醫(yī)學(xué)學(xué)院;河南農(nóng)業(yè)大學(xué);鄭州市豬重大疫病防控重點(diǎn)實(shí)驗(yàn)室;河南牧業(yè)經(jīng)濟(jì)學(xué)院;
【基金】：河南省產(chǎn)學(xué)研合作項(xiàng)目(132107000002、152107000003) 河南省科技攻關(guān)項(xiàng)目(162102110033、152102110092)
【分類號(hào)】：S852.651

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