【摘要】：東北細(xì)毛羊?yàn)槿饷嬗脙?yōu)良品種,本試驗(yàn)研究了東北細(xì)毛羊幼母羔超數(shù)排卵、卵母細(xì)胞成熟培養(yǎng)及體外受精的影響因素,旨在為該技術(shù)在生產(chǎn)實(shí)際中應(yīng)用提供相關(guān)借鑒。試驗(yàn)共分為三部分:分別研究了羔羊超數(shù)排卵效果影響因素、卵母細(xì)胞成熟液成熟效果和精子獲能方法及受精卵發(fā)育的影響因素,結(jié)果如下:1.6周齡羔羊回收卵枚數(shù)、回收率及可用卵枚數(shù)高于4周齡組和8周齡組,但差異不顯著(P0.05),可用卵率顯著高于4周齡組(P0.05);采用FSH+PMSG(加拿大產(chǎn))超排后回收率及可用卵率均高于其它三組,其中可用卵率極顯著高于FSH+LH(國產(chǎn))組(P0.01);秋季可用卵率高于春季并極顯著高于夏季(P0.01);首次超排卵母細(xì)胞回收率極顯著高于重復(fù)超排組(P0.01),可用卵率高于重復(fù)組但無顯著差異;非運(yùn)輸組可用卵率極顯著高于運(yùn)輸組(P0.01),卵回收率無明顯差異(P0.05)。2.比較了五種培養(yǎng)液的成熟效果,各組間成熟率差異不顯著(P0.05),表明可以使用10mg/ml BSA替代血清(10%FBS)的添加。3.相同獲能時(shí)間內(nèi),對照組獲能率顯著低于肝素添加組(P0.05),肝素添加組獲能2h獲能率達(dá)到峰值,并顯著高于對照組(P0.05);25μg/mL組精子存活時(shí)間顯著低于對照組(P0.05),同時(shí)顯著高于50μg/mL、100μg/mL添加組(P0.05);綜合結(jié)果表明,肝素濃度25μg/ml,處理2h的精子獲能效果最好。卵母細(xì)胞直徑130μm組第二極體率顯著高于d120μm組,卵裂率顯著大于120μmd130μm組(P0.05)并極顯著高于d120μm組(P0.01)。卵母細(xì)胞體外成熟培養(yǎng)24~27h第二極體率和卵裂率高于培養(yǎng)27~30h,極顯著高于培養(yǎng)21~24h(P0.01)。成熟液中添加EGF能夠顯著提高成熟率率和卵裂率(P0.05),極顯著提高囊胚率(P0.01),添加量為20 ng/mL較10 ng/mL囊胚率有極顯著提高(P0.01)。SOF體系卵裂率顯著高于TALP體系(P0.05),而SOF體系囊胚率極顯著高于TALP體系(P0.01)。
[Abstract]:%E4%B8%9C%E5%8C%97%E7%BB%86%E6%AF%9B%E7%BE%8A%E4%B8%BA%E8%82%89%E6%AF%9B%E5%85%BC%E7%94%A8%E4%BC%98%E8%89%AF%E5%93%81%E7%A7%8D%2C%E6%9C%AC%E8%AF%95%E9%AA%8C%E7%A0%94%E7%A9%B6%E4%BA%86%E4%B8%9C%E5%8C%97%E7%BB%86%E6%AF%9B%E7%BE%8A%E5%B9%BC%E6%AF%8D%E7%BE%94%E8%B6%85%E6%95%B0%E6%8E%92%E5%8D%B5%2C%E5%8D%B5%E6%AF%8D%E7%BB%86%E8%83%9E%E6%88%90%E7%86%9F%E5%9F%B9%E5%85%BB%E5%8F%8A%E4%BD%93%E5%A4%96%E5%8F%97%E7%B2%BE%E7%9A%84%E5%BD%B1%E5%93%8D%E5%9B%A0%E7%B4%A0%2C%E6%97%A8%E5%9C%A8%E4%B8%BA%E8%AF%A5%E6%8A%80%E6%9C%AF%E5%9C%A8%E7%94%9F%E4%BA%A7%E5%AE%9E%E9%99%85%E4%B8%AD%E5%BA%94%E7%94%A8%E6%8F%90%E4%BE%9B%E7%9B%B8%E5%85%B3%E5%80%9F%E9%89%B4.%E8%AF%95%E9%AA%8C%E5%85%B1%E5%88%86%E4%B8%BA%E4%B8%89%E9%83%A8%E5%88%86%3A%E5%88%86%E5%88%AB%E7%A0%94%E7%A9%B6%E4%BA%86%E7%BE%94%E7%BE%8A%E8%B6%85%E6%95%B0%E6%8E%92%E5%8D%B5%E6%95%88%E6%9E%9C%E5%BD%B1%E5%93%8D%E5%9B%A0%E7%B4%A0%2C%E5%8D%B5%E6%AF%8D%E7%BB%86%E8%83%9E%E6%88%90%E7%86%9F%E6%B6%B2%E6%88%90%E7%86%9F%E6%95%88%E6%9E%9C%E5%92%8C%E7%B2%BE%E5%AD%90%E8%8E%B7%E8%83%BD%E6%96%B9%E6%B3%95%E5%8F%8A%E5%8F%97%E7%B2%BE%E5%8D%B5%E5%8F%91%E8%82%B2%E7%9A%84%E5%BD%B1%E5%93%8D%E5%9B%A0%E7%B4%A0%2C%E7%BB%93%E6%9E%9C%E5%A6%82%E4%B8%8B%3A1.6%E5%91%A8%E9%BE%84%E7%BE%94%E7%BE%8A%E5%9B%9E%E6%94%B6%E5%8D%B5%E6%9E%9A%E6%95%B0%2C%E5%9B%9E%E6%94%B6%E7%8E%87%E5%8F%8A%E5%8F%AF%E7%94%A8%E5%8D%B5%E6%9E%9A%E6%95%B0%E9%AB%98%E4%BA%8E4%E5%91%A8%E9%BE%84%E7%BB%84%E5%92%8C8%E5%91%A8%E9%BE%84%E7%BB%84%2C%E4%BD%86%E5%B7%AE%E5%BC%82%E4%B8%8D%E6%98%BE%E8%91%97%28P0.05%29%2C%E5%8F%AF%E7%94%A8%E5%8D%B5%E7%8E%87%E6%98%BE%E8%91%97%E9%AB%98%E4%BA%8E4%E5%91%A8%E9%BE%84%E7%BB%84%28P0.05%29%3B%E9%87%87%E7%94%A8FSH%20PMSG%28%E5%8A%A0%E6%8B%BF%E5%A4%A7%E4%BA%A7%29%E8%B6%85%E6%8E%92%E5%90%8E%E5%9B%9E%E6%94%B6%E7%8E%87%E5%8F%8A%E5%8F%AF%E7%94%A8%E5%8D%B5%E7%8E%87%E5%9D%87%E9%AB%98%E4%BA%8E%E5%85%B6%E5%AE%83%E4%B8%89%E7%BB%84%2C%E5%85%B6%E4%B8%AD%E5%8F%AF%E7%94%A8%E5%8D%B5%E7%8E%87%E6%9E%81%E6%98%BE%E8%91%97%E9%AB%98%E4%BA%8EFSH%20LH%28%E5%9B%BD%E4%BA%A7%29%E7%BB%84%28P0.01%29%3B%E7%A7%8B%E5%AD%A3%E5%8F%AF%E7%94%A8%E5%8D%B5%E7%8E%87%E9%AB%98%E4%BA%8E%E6%98%A5%E5%AD%A3%E5%B9%B6%E6%9E%81%E6%98%BE%E8%91%97%E9%AB%98%E4%BA%8E%E5%A4%8F%E5%AD%A3%28P0.01%29%3B%E9%A6%96%E6%AC%A1%E8%B6%85%E6%8E%92%E5%8D%B5%E6%AF%8D%E7%BB%86%E8%83%9E%E5%9B%9E%E6%94%B6%E7%8E%87%E6%9E%81%E6%98%BE%E8%91%97%E9%AB%98%E4%BA%8E%E9%87%8D%E5%A4%8D%E8%B6%85%E6%8E%92%E7%BB%84%28P0.01%29%2C%E5%8F%AF%E7%94%A8%E5%8D%B5%E7%8E%87%E9%AB%98%E4%BA%8E%E9%87%8D%E5%A4%8D%E7%BB%84%E4%BD%86%E6%97%A0%E6%98%BE%E8%91%97%E5%B7%AE%E5%BC%82%3B%E9%9D%9E%E8%BF%90%E8%BE%93%E7%BB%84%E5%8F%AF%E7%94%A8%E5%8D%B5%E7%8E%87%E6%9E%81%E6%98%BE%E8%91%97%E9%AB%98%E4%BA%8E%E8%BF%90%E8%BE%93%E7%BB%84%28P0.01%29%2C%E5%8D%B5%E5%9B%9E%E6%94%B6%E7%8E%87%E6%97%A0%E6%98%8E%E6%98%BE%E5%B7%AE%E5%BC%82%28P0.05%29.2.%E6%AF%94%E8%BE%83%E4%BA%86%E4%BA%94%E7%A7%8D%E5%9F%B9%E5%85%BB%E6%B6%B2%E7%9A%84%E6%88%90%E7%86%9F%E6%95%88%E6%9E%9C%2C%E5%90%84%E7%BB%84%E9%97%B4%E6%88%90%E7%86%9F%E7%8E%87%E5%B7%AE%E5%BC%82%E4%B8%8D%E6%98%BE%E8%91%97%28P0.05%29%2C%E8%A1%A8%E6%98%8E%E5%8F%AF%E4%BB%A5%E4%BD%BF%E7%94%A810mg%2Fml%20BSA%E6%9B%BF%E4%BB%A3%E8%A1%80%E6%B8%85%2810%FBS%29%E7%9A%84%E6%B7%BB%E5%8A%A0.3.%E7%9B%B8%E5%90%8C%E8%8E%B7%E8%83%BD%E6%97%B6%E9%97%B4%E5%86%85%2C%E5%AF%B9%E7%85%A7%E7%BB%84%E8%8E%B7%E8%83%BD%E7%8E%87%E6%98%BE%E8%91%97%E4%BD%8E%E4%BA%8E%E8%82%9D%E7%B4%A0%E6%B7%BB%E5%8A%A0%E7%BB%84%28P0.05%29%2C%E8%82%9D%E7%B4%A0%E6%B7%BB%E5%8A%A0%E7%BB%84%E8%8E%B7%E8%83%BD2h%E8%8E%B7%E8%83%BD%E7%8E%87%E8%BE%BE%E5%88%B0%E5%B3%B0%E5%80%BC%2C%E5%B9%B6%E6%98%BE%E8%91%97%E9%AB%98%E4%BA%8E%E5%AF%B9%E7%85%A7%E7%BB%84%28P0.05%29%3B25%CE%BCg%2FmL%E7%BB%84%E7%B2%BE%E5%AD%90%E5%AD%98%E6%B4%BB%E6%97%B6%E9%97%B4%E6%98%BE%E8%91%97%E4%BD%8E%E4%BA%8E%E5%AF%B9%E7%85%A7%E7%BB%84%28P0.05%29%2C%E5%90%8C%E6%97%B6%E6%98%BE%E8%91%97%E9%AB%98%E4%BA%8E50%CE%BCg%2FmL%2C100%CE%BCg%2FmL%E6%B7%BB%E5%8A%A0%E7%BB%84%28P0.05%29%3B%E7%BB%BC%E5%90%88%E7%BB%93%E6%9E%9C%E8%A1%A8%E6%98%8E%2C%E8%82%9D%E7%B4%A0%E6%B5%93%E5%BA%A625%CE%BCg%2Fml%2C%E5%A4%84%E7%90%862h%E7%9A%84%E7%B2%BE%E5%AD%90%E8%8E%B7%E8%83%BD%E6%95%88%E6%9E%9C%E6%9C%80%E5%A5%BD.%E5%8D%B5%E6%AF%8D%E7%BB%86%E8%83%9E%E7%9B%B4%E5%BE%84130%CE%BCm%E7%BB%84%E7%AC%AC%E4%BA%8C%E6%9E%81%E4%BD%93%E7%8E%87%E6%98%BE%E8%91%97%E9%AB%98%E4%BA%8Ed120%CE%BCm%E7%BB%84%2C%E5%8D%B5%E8%A3%82%E7%8E%87%E6%98%BE%E8%91%97%E5%A4%A7%E4%BA%8E120%CE%BCmd130%CE%BCm%E7%BB%84%28P0.05%29%E5%B9%B6%E6%9E%81%E6%98%BE%E8%91%97%E9%AB%98%E4%BA%8Ed120%CE%BCm%E7%BB%84%28P0.01%29.%E5%8D%B5%E6%AF%8D%E7%BB%86%E8%83%9E%E4%BD%93%E5%A4%96%E6%88%90%E7%86%9F%E5%9F%B9%E5%85%BB24~27h%E7%AC%AC%E4%BA%8C%E6%9E%81%E4%BD%93%E7%8E%87%E5%92%8C%E5%8D%B5%E8%A3%82%E7%8E%87%E9%AB%98%E4%BA%8E%E5%9F%B9%E5%85%BB27~30h%2C%E6%9E%81%E6%98%BE%E8%91%97%E9%AB%98%E4%BA%8E%E5%9F%B9%E5%85%BB21~24h%28P0.01%29.%E6%88%90%E7%86%9F%E6%B6%B2%E4%B8%AD%E6%B7%BB%E5%8A%A0EGF%E8%83%BD%E5%A4%9F%E6%98%BE%E8%91%97%E6%8F%90%E9%AB%98%E6%88%90%E7%86%9F%E7%8E%87%E7%8E%87%E5%92%8C%E5%8D%B5%E8%A3%82%E7%8E%87%28P0.05%29%2C%E6%9E%81%E6%98%BE%E8%91%97%E6%8F%90%E9%AB%98%E5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【學(xué)位授予單位】：吉林農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S826

【參考文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前1條

1 武建朝;利用性成熟前山羊卵泡卵母細(xì)胞體外生產(chǎn)胚胎的研究[D];南京農(nóng)業(yè)大學(xué);2007年

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