【摘要】：試驗(yàn)旨在建立豬圓環(huán)病毒2型(PCV2)誘導(dǎo)的小鼠體內(nèi)免疫細(xì)胞氧化脅迫模型。篩選PCV2感染劑量及感染時(shí)間,采用腹腔注射、滴鼻和灌胃3種途徑聯(lián)合方式于第1、2、3天或第1、3、5、7天給予昆明系小鼠感染PCV2病毒原液或10-1 PCV2病毒液,分別于感染后7、14、21d剖殺小鼠,測定活性氧(ROS)、總谷胱甘肽(T-GSH)、還原型谷胱甘肽(GSH)、氧化型谷胱甘肽(GSSG)水平及黃嘌呤氧化酶(XOD)、髓過氧化物酶(MPO)、誘導(dǎo)型一氧化氮合酶(iNOS)活性,探討PCV2感染時(shí)間與活性氧水平變化的關(guān)系,建立免疫細(xì)胞氧化脅迫動物模型。結(jié)果顯示,第1、2、3天每天經(jīng)3種途徑聯(lián)合感染PCV2病毒原液,1mL/只,為后續(xù)試驗(yàn)感染最佳方案。PCV2感染小鼠3個時(shí)間點(diǎn)細(xì)胞內(nèi)ROS水平較空白對照組均極顯著升高(P0.01),感染后7、14dGSH水平顯著降低(P0.05);感染后7dGSSG水平極顯著高于空白對照組(P0.01);感染后7dT-GSH水平顯著低于空白對照組(P0.05),感染后14dT-GSH水平極顯著低于空白對照組(P0.01)。感染后7d脾臟XOD、MPO、iNOS活性與空白對照組相比均存在顯著差異(P0.05)。結(jié)果表明,PCV2成功感染小鼠,試驗(yàn)感染方案為第1、2、3天每天經(jīng)3種途徑聯(lián)合感染PCV2病毒原液,1mL/只,且用PCV2病毒原液感染7d是建立小鼠體內(nèi)免疫細(xì)胞氧化脅迫模型的最佳條件。
[Abstract]:The aim of this study was to establish an oxidative stress model of immune cells induced by porcine circovirus type 2 (PCV2) in mice. The dose and time of PCV2 infection were screened, and the mice were killed by intraperitoneal injection, nasal drip and intragastric administration on the 3rd day of the first day of infection or on the 1st day of the 3rd day or the 7th day of the first day of infection with PCV2 virus or 10-1 PCV2 virus solution. The mice were killed on the 7th day, 14th day, 21d after infection, respectively. The levels of total glutathione (T-GSH), reduced glutathione (GSH), oxidized glutathione (GSSG) and (MPO), inducible nitric oxide synthase (iNOS) activity of (XOD), myeloperoxidase of xanthine oxidase were measured in order to explore the relationship between the infection time of PCV2 and the level of reactive oxygen species. The animal model of oxidative stress of immune cells was established. The results showed that 1 mL / mouse of PCV2 virus was co-infected by three ways every day on the first day of the second day. The level of intracellular ROS in PCV2 infected mice was significantly higher than that in the control group (P0.01), the level of GSH was significantly decreased at 7d after infection (P0.05), the level of 7dGSSG after infection was significantly higher than that of the control group (P0.01), and the level of 7dGSSG was significantly higher in PCV2 infected mice than in the control group (P0.01). The level of 7dT-GSH after infection was significantly lower than that of control group (P0.05), and the level of 14dT-GSH after infection was significantly lower than that of blank control group (P0.01). There were significant differences in spleen XOD,MPO,iNOS activity between the control group and the control group 7 days after infection (P0.05). The results showed that PCV2 was successfully infected in mice, and the optimal condition for the establishment of oxidative stress model of immune cells in mice was established by using PCV2 virus solution for 7 days.
【作者單位】： 廣西大學(xué)動物科學(xué)技術(shù)學(xué)院;
【基金】：國家自然科學(xué)基金資助項(xiàng)目(31260619) 高等學(xué)校博士學(xué)科點(diǎn)專項(xiàng)科研基金博導(dǎo)類資助課題(20134501110004) 廣西研究生教育創(chuàng)新計(jì)劃資助項(xiàng)目(YCSZ2015038) 廣西研究生教育創(chuàng)新計(jì)劃項(xiàng)目學(xué)位與研究生教育改革專項(xiàng)課題(JGY2015002)
【分類號】：S852.651

【參考文獻(xiàn)】

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1 林丹丹;秦韜;任U，

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