【摘要】：試驗旨在研究魚油飼糧中止痢草油添加量對公豬精子形態(tài)、精子膜及抗氧化作用的影響。選用34頭健康成熟公豬,隨機分為4組,分別飼喂添加100 mg/kg維生素E(1組,n=8)和250(2組,n=8)、500(3組,n=9)、750 mg/kg(4組,n=9)的止痢草油飼糧,試驗期16周。結果表明:在整個試驗期,試驗3組的精子遠端原生質滴比例低于1組(P0.05),試驗3組在16周的精子正常形態(tài)分別較試驗1和4組有極顯著提高(P0.01);在整個試驗期,試驗2、3和4組精清超氧化物歧化酶活性均高于1組(P0.05);試驗2和3組膜結構完整性在12周較4組有顯著提高,而試驗3組膜結構完整性和線粒體膜電位在16周較1和4組有顯著提高(P0.05)。結果提示,魚油飼糧添加止痢草油可增強公豬血清和精清抗氧化能力,添加500 mg/kg止痢草油有利于精子膜的改善,從而改善了精子形態(tài)。
[Abstract]:The purpose of this study was to study the effects of fish oil diet on sperm morphology, sperm membrane and antioxidation of boars. Thirty-four healthy mature boars were randomly divided into 4 groups, fed with 100 mg/kg vitamin E (group 1) and 250 (group 2) (group 1) and group 2 (group 2) respectively. The diets were fed with 100 mg/kg vitamin E (group 1) and mg/kg (group 3) for 16 weeks. The results showed that during the whole trial period, the percentage of protoplast droplets at the distal end of spermatozoa in group 3 was lower than that in group 1 (P0.05), and the normal morphology of spermatozoa in group 3 was significantly higher than that in group 1 and group 4 at 16 weeks (P0.01). The activity of superoxide dismutase (SOD) in spermatozoa of group 2 and group 4 was higher than that of group 1 (P0.05), and the structural integrity of membrane in groups 2 and 3 was significantly higher than that in group 4 at 12 weeks. However, the membrane structural integrity and mitochondrial membrane potential were significantly higher in experimental group 3 than in groups 1 and 4 at 16 weeks (P0.05). The results showed that the antioxidation ability of serum and seminal plasma of boars was enhanced by adding the diet of fish oil, and the sperm membrane was improved by adding 500 mg/kg antipullorum oil, and the morphology of sperm was improved.
【作者單位】： 華中農業(yè)大學動物科技學院動物營養(yǎng)與飼料科學系;生豬健康養(yǎng)殖協(xié)同創(chuàng)新中心;
【基金】：國家科技支撐計劃(2014BAD20B01) 湖北省農業(yè)創(chuàng)新崗位(2007-620) 湖北省自然科學基金(2013CFA010) 自主科技創(chuàng)新基金培育專項(2662015PY014)
【分類號】：S828.5

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