【摘要】：【目的】為了探討不同滅活時(shí)間及純化對(duì)多型口蹄疫疫苗146 S、總蛋白量、抗原收獲量及疫苗效力的影響�！痉椒ā窟x取OM/Re-A/AF/Asia1 4種不同型的口蹄疫疫苗為研究對(duì)象,設(shè)定不同滅活時(shí)間(8、10、12、14和16 h),對(duì)每個(gè)時(shí)間點(diǎn)疫苗母液中146 S、總蛋白和抗原收獲量進(jìn)行檢測(cè)和差異性分析;測(cè)量純化前后146 S和總蛋白的變化趨勢(shì);測(cè)定不同滅活時(shí)間及純化前后疫苗PD50數(shù)值進(jìn)行判定效力�！窘Y(jié)果】滅活不同時(shí)間點(diǎn),146 S含量和抗原收獲量隨著時(shí)間先增高后平緩的趨勢(shì),146 S在14 h出現(xiàn)平緩,而抗原收獲量在10 h時(shí)開(kāi)始平緩;146 S含量OM和AF型明顯高于Re-A和Asia1型,抗原收獲量Asia1型最高;總蛋白含量隨著時(shí)間呈現(xiàn)先降低后平緩的趨勢(shì),AF型口蹄疫疫苗降低最明顯;疫苗純化前后相比分析發(fā)現(xiàn),146 S含量明顯升高,總蛋白含量明顯降低(P0.01)。4種疫苗PD50值隨著純化時(shí)間先增大后平穩(wěn),純化后PD50顯著提高(P0.01),免疫效力增強(qiáng)�！窘Y(jié)論】OM/Re-A/AF/Asia1 4種不同型的口蹄疫疫苗在純化不同時(shí)間點(diǎn),疫苗中146 S、抗原收獲量和總蛋白含量呈規(guī)律性變化,且純化前后146 S、總蛋白含量及PD50值差異性極顯著,表明口蹄疫疫苗的滅活時(shí)間和純化是影響疫苗效價(jià)的重要因素。
[Abstract]:[objective] to investigate the effects of different inactivation time and purification on the amount of protein, total protein, antigen harvest and vaccine effectiveness of multiform foot-and-mouth disease vaccine 146S. [methods] four different types of foot-and-mouth disease vaccine (OM/Re-A/AF/Asia1) were selected as the research object. Different inactivation time (810) 12C 14 and 16 h), were set to detect and analyze the yield of 146s, total protein and antigen in the maternal liquor of vaccine at each time point, and the change trend of 146S and total protein before and after purification were measured. [results] the trend of inactivation of 146S and antigen harvest at different time points was that the level of 146S and then the yield of antigen increased first and then slowly at 14 h after inactivation, the efficacy was determined by measuring the different inactivation time and the PD50 value of the vaccine before and after purification. However, the OM and AF types were significantly higher than those of Re-A and Asia1 at 10 h, and the total protein content was the highest in Asia1 type, and the total protein content decreased first and then slowly. The decrease of AF type foot-and-mouth disease vaccine was the most obvious. Compared with those before and after purification, the results showed that the content of total protein was significantly decreased (P0.01), and the PD50 value of the vaccine increased first and then stabilized with the purification time. After purification, PD50 was significantly increased (P0.01), and the immune potency was enhanced. [conclusion] at different time points of purification, 146s, antigen harvest and total protein content of OM/Re-A/AF/Asia1 four different types of FMD vaccine showed regular changes. There were significant differences in total protein content and PD50 before and after purification, indicating that the inactivation time and purification of foot-and-mouth disease vaccine were important factors affecting the titer of the vaccine.
【作者單位】： 甘肅農(nóng)業(yè)大學(xué)動(dòng)物醫(yī)學(xué)院;
【基金】：國(guó)家自然基金項(xiàng)目“冷應(yīng)激因子CIRP和HSP70與牦牛高寒低氧環(huán)境的適應(yīng)性機(jī)制”(31360594)
【分類號(hào)】：S852.4

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