【摘要】：本文以課題組前期研制的替代飼用抗生素的復(fù)方中草藥提取物為原料,研究該提取物及其組成的各單味藥的體外清除自由基功能、體內(nèi)抗氧化功能,并對(duì)其抗氧化成分進(jìn)行初步測(cè)定分析,探索其抗氧化機(jī)制,為進(jìn)一步開(kāi)發(fā)利用飼用抗生素的替代物提供理論依據(jù)。1復(fù)方中草藥提取物清除自由基活性研究。通過(guò)體外抗氧化試驗(yàn)研究復(fù)方中草藥提取物及其組成的各單味藥對(duì)DPPH·自由基、羥自由基的清除作用,并測(cè)定了其還原能力。結(jié)果顯示:復(fù)方中草藥提取物及其各單味藥對(duì)DPPH·自由基、羥自由基均具有較強(qiáng)的清除能力,但同一種藥對(duì)不同自由基的清除效果不同。其中,對(duì)DPPH·自由基的清除作用強(qiáng)弱順序?yàn)?五倍子復(fù)方1香薷野菊花烏梅大青葉復(fù)方2柴胡;對(duì)羥自由基的清除作用強(qiáng)弱順序?yàn)?五倍子烏梅復(fù)方1復(fù)方2大青葉野菊花香薷柴胡;在所測(cè)定濃度范圍內(nèi),復(fù)方中草藥提取物及其各單味藥還原能力隨濃度的增加而增大。在相同濃度下,中草藥提取物的還原能力大小順序?yàn)?五倍子復(fù)方1野菊花香薷烏梅復(fù)方2大青葉柴胡。2復(fù)方中草藥提取物體內(nèi)抗氧化活性研究。通過(guò)動(dòng)物試驗(yàn)研究以烏梅為主要成分的復(fù)方中草藥提取物對(duì)小鼠外周血、心臟、肝臟、脾臟、腎臟中抗氧化酶SOD、GSH-PX活力及脂質(zhì)過(guò)氧化物MDA含量的影響。結(jié)果顯示:復(fù)方2對(duì)小鼠機(jī)體具有較強(qiáng)的抗氧化能力。該提取物在100、200、400mg/kg·bw三個(gè)劑量水平上均可導(dǎo)致小鼠外周血、心、肝、脾、腎組織中SOD和GSH-Px活性顯著增強(qiáng),降低小鼠上述組織中MDA含量,并呈現(xiàn)劑量反應(yīng)關(guān)系。其中,在200mg/kg·bw劑量下,上述組織SOD活性依次提高了46.44%、26.55%、55.15%、34.71%、83.74%,GSH-PX活性依次提高了17.23%、0.58%、44.57%、49.54%、22.49%,MDA含量依次下降了13.65%、34.42%、11.60%、16.63%、28.73%。3復(fù)方中草藥提取物抗氧化活性成分分析。通過(guò)紫外顯色反應(yīng)測(cè)定各復(fù)方中草藥提取物及其各單味藥中多酚及黃酮的含量。結(jié)果顯示:復(fù)方中草藥提取物及其各單味藥中多酚含量從高到低依次為:五倍子復(fù)方1香薷復(fù)方2野菊花烏梅大青葉柴胡,黃酮含量從高到低依次為:為五倍子香薷復(fù)方1復(fù)方2野菊花烏梅大青葉柴胡。其中五倍子總酚和黃酮含量最高,分別為30.59ug/mg、58.47ug/mg。復(fù)方中草藥提取物及其各單味藥對(duì)DPPH·自由基IC50值與總酚含量的相關(guān)系數(shù)r=-0.422;與黃酮含量的相關(guān)系數(shù)r=-0.609。復(fù)方中草藥提取物及其各單味藥對(duì)·OH自由基IC50值與總酚含量的相關(guān)系數(shù)r=-0.443;與黃酮含量的相關(guān)系數(shù)r=-0.506。由復(fù)方中草藥提取物及其各單味藥對(duì)DPPH·自由基、·OH自由基的IC50值與總酚或黃酮含量的相關(guān)系數(shù)可知,其對(duì)DPPH·和·OH自由基呈中度相關(guān),說(shuō)明除了多酚、黃酮外,中草藥對(duì)自由基清除作用還與其所含的其他成分有關(guān)。結(jié)論:復(fù)方中草藥提取物及其各單味藥對(duì)DPPH·自由基、羥自由基均具有較強(qiáng)的清除能力,在所測(cè)定濃度范圍內(nèi),復(fù)方中草藥提取物及其各單味藥還原能力隨濃度的增加而增大;復(fù)方2對(duì)小鼠機(jī)體具有較強(qiáng)的抗氧化能力;復(fù)方中草藥提取物及其各單味藥具有清除自由基的作用與其多酚、黃酮的含量呈中度相關(guān)。
[Abstract]:In this paper, the compound Chinese herbal medicine extract, which was developed by our research group as a substitute for feeding antibiotics, was used as raw material to study the free radical scavenging function in vitro and antioxidant function in vivo of the extract and its constituents. The antioxidant components were determined and analyzed preliminarily, and the antioxidant mechanism was explored to further develop and utilize feeding antibiotics. The scavenging effect of compound Chinese herbal extracts and their constituents on DPPH free radicals and hydroxyl free radicals was studied by antioxidant test in vitro, and their reducing ability was determined. The scavenging effect of the same drug on DPPH free radicals was different. The order of scavenging effect on DPPH free radicals was as follows: Galla Chinensis Compound 1 Fragrant Elsholtzia Wild Chrysanthemum Flower, Mume and Daqing Leaf Compound 2 Bupleurum; the order of scavenging effect on hydroxyl radicals was as follows: Galla Chinensis Blume. Compound 1, Compound 1, Compound 2, Compound 1, Compound 1, Compound 2, Compound 2, Compound 2, Compound 2, Compound 2, Compound 2, Compound 2, Compound 2, Compound 2, Compound 2, Compound Chinese Herbal Medicine, Compound 1, Compound 1, Compound 1, Compound 1, Compound 2, Compound 2, Compound 2, Compound 2, Compound 2, Compound 2, Compound Chinese Herbal Medicine, Compound 1, Compo The antioxidant activity of the extract in vivo was studied.The antioxidant enzyme activities of SOD,GSH-PX and the content of MDA in peripheral blood,heart,liver,spleen and kidney of mice were studied by animal experiment.The results showed that compound 2 had strong antioxidant activity in mice. The activity of SOD and GSH-Px in peripheral blood, heart, liver, spleen and kidney tissues of mice were significantly increased at 100,200,400 mg/kg/bw levels, and the MDA content in the above tissues of mice was decreased in dose-response relationship. The SOD activity in the above tissues was increased by 46.44%, 26.55%, 55.15%, 34.71%, 83.71% at 200 mg/kg/bw dosage. The activity of GSH-PX increased by 17.23%, 0.58%, 44.57%, 49.54%, 22.49%, and the content of MDA decreased by 13.65%, 34.42%, 11.60%, 16.63% and 28.73% respectively. 3 Compound Chinese herbal extracts were analyzed for their antioxidant activity components. The content of polyphenols and flavonoids in each compound Chinese herbal extract and its single herbal medicine was determined by UV reaction. The contents of polyphenols in the extracts of Chinese herbal medicines and their single herbs were as follows: Galla Chinensis Compound Prescription 1 Xiangqiao Compound Prescription 2 Wild Chrysanthemum Black Plum Daqing Leaf Bupleurum. The contents of flavonoids were as follows: Galla Chinensis Compound Prescription 1 Wild Chrysanthemum Compound Prescription 2 Wild Chrysanthemum Black Plum Blue Leaf Bupleurum. 47ug/mg. The correlation coefficient between the IC50 value of DPPH radical and the content of total phenols of the extracts of Chinese herbal medicines and their single herbs was r = - 0.422; the correlation coefficient with the content of flavonoids was r = - 0.609. The correlation coefficient between the IC50 value of OH radical and the content of total phenols of the extracts of Chinese herbal medicines and their single herbs was r = - 0.443; the correlation coefficient with the content of flavonoids was r = - 0.506. The correlation coefficients between the IC50 values of DPPH free radicals and OH free radicals and the contents of total phenols or flavonoids showed that the IC50 values were moderately correlated with DPPH and OH free radicals, indicating that the scavenging effect of Chinese herbal medicine on free radicals was related to other components besides polyphenols and flavonoids. The extracts and their individual medicines have strong scavenging capacity on DPPH free radicals and hydroxyl free radicals. Within the range of concentration determined, the reducing capacity of compound Chinese herbal extracts and their individual medicines increases with the increase of concentration; compound 2 has strong antioxidant capacity in mice; compound Chinese herbal extracts and their individual medicines have strong antioxidant capacity. The scavenging effect of free radicals was moderately related to polyphenols and flavonoids.
【學(xué)位授予單位】：河北農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S853.7

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 馮東輝;褚宏山;曹露露;王立明;陳傳千;沈艷平;馮波;;比色法測(cè)定野菊花中總黃酮含量[J];吉林醫(yī)藥學(xué)院學(xué)報(bào);2009年05期

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本文編號(hào)：2247879