【摘要】：CD9是一個(gè)腫瘤相關(guān)因子,早期的研究證實(shí)CD9的表達(dá)能夠抑制腫瘤的生長(zhǎng)和轉(zhuǎn)移,后來有研究人員提出CD9表達(dá)量在腫瘤細(xì)胞中升高,并證實(shí)其表達(dá)促進(jìn)腫瘤的發(fā)展,因此CD9在腫瘤發(fā)展過程中的角色仍然存在巨大爭(zhēng)議。鹿茸再生過程中,細(xì)胞增殖速度甚至超過腫瘤,CD9同樣在鹿茸干細(xì)胞中高表達(dá)。為了探討鹿茸干細(xì)胞中CD9的功能,本研究利用Western blot,免疫熒光和流式細(xì)胞儀分析技術(shù)系統(tǒng)地檢測(cè)了鹿茸干細(xì)胞(包括生茸區(qū)骨膜細(xì)胞(antlerogenic periosteum cells,APC),角柄骨膜細(xì)胞(pedicle periosteum cells,PPC)和鹿茸尖部間充質(zhì)細(xì)胞(reserve mesenchymal cells,RMC))中CD9的表達(dá)情況;并利用Anti-CD9抗體抑制CD9活性,檢測(cè)了CD9對(duì)鹿茸干細(xì)胞遷移和增殖的影響。結(jié)果顯示,3種鹿茸干細(xì)胞均高水平表達(dá)CD9,原代培養(yǎng)的鹿茸干細(xì)胞CD9陽性率超過90%;抑制CD9活性,對(duì)鹿茸干細(xì)胞的遷移影響不顯著,但對(duì)部分鹿茸干細(xì)胞(PPC和鹿茸R(shí)MC)的增殖有促進(jìn)作用;分析了CD9有關(guān)的細(xì)胞基質(zhì)-整合素和細(xì)胞粘附信號(hào)通路,并從鹿茸干細(xì)胞轉(zhuǎn)錄組中檢測(cè)到絕大多數(shù)因子。本研究揭示了CD9在鹿茸干細(xì)胞中的功能并推測(cè)了可能的調(diào)控機(jī)制,將有助于揭示CD9在快速增殖的細(xì)胞特別是腫瘤細(xì)胞中的作用。
[Abstract]:CD9 is a tumor-related factor. Early studies have shown that the expression of CD9 can inhibit the growth and metastasis of tumor. Later, some researchers suggested that the expression of CD9 increased in tumor cells and promoted the development of tumor. Therefore, the role of CD9 in tumor development is still controversial. In the process of pilose antler regeneration, the cell proliferation rate was even higher than that of tumor CD9 expression in pilose antler stem cells. In order to investigate the function of CD9 in pilose antler stem cells, In this study, Western blot, immunofluorescence and flow cytometry were used to detect the expression of CD9 in pilose antler stem cells (including (antlerogenic periosteum cells,APC, (pedicle periosteum cells,PPC and (reserve mesenchymal cells,RMC). The effect of CD9 on migration and proliferation of pilose antler stem cells was detected by using Anti-CD9 antibody to inhibit CD9 activity. The results showed that the positive rate of CD9 of the three kinds of pilose antler stem cells expressing CD9, was higher than 90%, and the inhibition of CD9 activity had no significant effect on the migration of pilose antler stem cells. But it can promote the proliferation of some pilose antler stem cells (PPC and RMC), analyze the cell matrix integrin and cell adhesion signaling pathway related to CD9, and detect most of the factors from the transcription group of pilose antler stem cells. This study revealed the function of CD9 in pilose antler stem cells and speculated the possible regulatory mechanism, which will help to reveal the role of CD9 in rapidly proliferating cells, especially in tumor cells.
【作者單位】： 中國(guó)農(nóng)業(yè)科學(xué)院特產(chǎn)研究所/特種經(jīng)濟(jì)動(dòng)物分子生物學(xué)國(guó)家重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(No.31500792) 吉林省自然科學(xué)基金(No.20170101032JC) 中國(guó)農(nóng)業(yè)科學(xué)院科技創(chuàng)新工程項(xiàng)目(No.CAAS-ASTIP-2014-ISAPS-04)
【分類號(hào)】：S825

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