【摘要】：本課題的研究目的是利用PCR方法對(duì)延邊地區(qū)不同縣市總計(jì)320頭尚未注射預(yù)防布氏桿菌病疫苗的豬進(jìn)行檢測(cè),對(duì)豬布病的流行情況進(jìn)行流行病學(xué)調(diào)查,從而為延邊地區(qū)豬布病的防控提供科學(xué)參考。在研究過(guò)程中,對(duì)320頭抽檢豬首先使用琥紅平板凝集檢測(cè)方法進(jìn)行初步檢測(cè),再使用試管凝集方法進(jìn)一步檢測(cè),最終利用PCR方法對(duì)被檢豬進(jìn)行豬布病流行病學(xué)調(diào)查,以統(tǒng)計(jì)延邊地區(qū)不同縣市豬布病的流行情況。試驗(yàn)檢測(cè)結(jié)果如下:1.RBPT檢測(cè)陽(yáng)性率約為31.3%(100/320),陰性率約為68.7%(220/320);和龍市陽(yáng)性率最高為36.7%(22/60),敦化市陽(yáng)性率最低為20.0%(6/30)。2.SAT檢測(cè)陽(yáng)性率約為21.3(68/320),陰性率約為78.7%(252/320);和龍市陽(yáng)性率最高為26.7%(16/60),延吉市陽(yáng)性率最低為10.0%(2/20)。3.PCR檢測(cè)陽(yáng)性率約為13.8%(44/320),陰性率約為86.2%(276/320);和龍市陽(yáng)性率最高為16.7%(10/50),延吉市陽(yáng)性率最低為5.0%(1/20)。4.抽檢200只圈養(yǎng)豬,RBPT檢測(cè)陽(yáng)性率為32.0%(64/200),SAT檢測(cè)陽(yáng)性率為25.0%(50/200),PCR檢測(cè)陽(yáng)性率為 17.0%(34/200)。5.抽檢120只散養(yǎng)豬,RBPT檢測(cè)陽(yáng)性率為30.0%(36/120),SAT檢測(cè)陽(yáng)性率為15.0%(18/120),PCR 檢測(cè)陽(yáng)性率為 8.3%(10/120)。6.夏季抽檢的198只豬中,和龍市、敦化市檢測(cè)陽(yáng)性率相同為22.2%,汪清縣為18.8%,圖們市、龍井市以及安圖縣陽(yáng)性率相同為16.7%,琿春市為12.5%,延吉市為8.3%(1/12);198頭份豬全血PCR檢測(cè)總陽(yáng)性率為17.2%(34/198)。7.冬季抽檢的122只豬中,龍井市檢測(cè)陽(yáng)性率為14.3%,圖們市、安圖縣陽(yáng)性率相同為12.5%,汪清縣為11.1%,和龍市為8.3%,延吉市、敦化市、琿春市陽(yáng)性率相同為0;61頭份豬全血PCR檢測(cè)總陽(yáng)性率為8.2%(10/122)。8.利用PCR方法對(duì)延邊地區(qū)部分豬場(chǎng)320頭豬進(jìn)行布病流行病學(xué)調(diào)查后,可以確定延邊地區(qū)是豬布病的流行地區(qū),且和龍市流行最嚴(yán)重,而且夏季陽(yáng)性率高于冬季,圈養(yǎng)陽(yáng)性率高于散養(yǎng)。
[Abstract]:The purpose of this study was to investigate the prevalence of brucellosis by using PCR method in 320 pigs from different counties and cities in Yanbian area which had not been vaccinated against brucellosis. So as to provide scientific reference for prevention and control of pig cloth disease in Yanbian area. In the course of the study, 320 pig samples were detected by using the method of hunoon-red plate agglutination first, and then further detected by the method of test tube agglutination. Finally, the epidemiological investigation of pig brucellosis was carried out by using PCR method. The epidemic situation of pig cloth disease in different counties in Yanbian area was analyzed. The results are as follows: 1. The positive rate of RBPT is about 31.3% (100 / 320), the negative rate is about 68.7% (220 / 320), the highest positive rate is 36.7% (22 / 60) in Hulong City, the lowest positive rate is 20.0% (630 / 30) .2.SAT positive rate is about 21.3% (630 / 320) in Dunhua City, and 78.7% (252 / 320) in Helong City, 26.7% (16 / 60) in Harong City, and 26.7% (16 / 60) in Yanjin City. The lowest positive rate was 10.0% (2 / 20) .3.The positive rate of PCR was about 13.8% (44 / 320), the negative rate was about 86.2% (276 / 320), the highest positive rate of Helong was 16.7% (10 / 50), and the lowest positive rate of Yanji was 5.0% (1 / 20) .4. The positive rate of RBPT was 32.0% (64 / 200). The positive rate of SAT was 25.0% (50 / 200). The positive rate of PCR was 17.0% (34 / 200) .5. The positive rate of RBPT was 30.0% (36 / 120). The positive rate of SAT was 15.0% (18 / 120). The positive rate of PCR was 8.3% (10 / 120) .6. Among 198 pigs sampled in summer, the positive rate in Helong City and Dunhua City was the same as that in Wangqing County (18.8%), Tumen City, Longjing City and Antu County were 16.7%, Hunchun City 12.5%, Yanji City 8.3% (1 / 12) 198 whole Blood PCR positive rate 17.2% (34 / 198) .7. Of the 122 pigs sampled in winter, the positive rate in Longjing City was 14.33.The positive rate in Tumen and Antu counties was 12.5. the positive rate was 11.1cm in Wangqing County, 8.3in Helong city, 8.3in Yanji city, Dunhua city and Hunchun city. The total positive rate of whole blood PCR test was 8.2% (10 / 122) .8. Based on the epidemiological investigation of brucellosis in some pig farms in Yanbian area by PCR method, it can be concluded that Yanbian area is the endemic area of swine brucellosis, and the epidemic is the most serious in Helong city, and the positive rate in summer is higher than that in winter. The positive rate in captivity was higher than that in rest.
【學(xué)位授予單位】：延邊大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：S858.28
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本文編號(hào)：2223332