【摘要】：極低密度脂蛋白受體基因(VLDLR)在卵生動物蛋黃物質(zhì)形成過程起關(guān)鍵作用。研究以國紹Ⅰ號母鴨為研究對象,采用一代測序法對VLDLR基因外顯子12至外顯子13區(qū)域進(jìn)行SNPs篩選,并分析各多態(tài)位點(diǎn)間的連鎖不平衡狀態(tài)及不同基因型與蛋品質(zhì)的相關(guān)性。結(jié)果顯示:共發(fā)現(xiàn)4個(gè)多態(tài)位點(diǎn),其中S1(A7120G)位于12號外顯子,未引起氨基酸序列改變;S2(A7261G)位于12號內(nèi)含子;S3(C7325T)和S4(A7337G)位于13號外顯子中,分別導(dǎo)致Thr突變?yōu)镸et,Asn突變?yōu)锳sp。位點(diǎn)S1、S2和S4為強(qiáng)連鎖不平衡位點(diǎn),存在3種單倍型和3種雙倍型,單一位點(diǎn)基因型和雙倍型與蛋黃比例、蛋重、蛋黃重、蛋殼重和蛋黃顏色的相關(guān)性分析結(jié)果均為不顯著相關(guān),在國紹Ⅰ號VLDLR基因外顯子12-外顯子13區(qū)域內(nèi)未發(fā)現(xiàn)與蛋黃比例等蛋品質(zhì)相關(guān)的分子標(biāo)記SNPs位點(diǎn)。
[Abstract]:Very low density lipoprotein receptor (VLDLR) gene plays a key role in egg yolk formation. In this study, the first generation sequencing method was used to screen the region of exon 12 to exon 13 of VLDLR gene, and the linkage disequilibrium state among the polymorphic loci and the correlation between different genotypes and egg quality were analyzed. The results showed that four polymorphic loci were found, of which S1 (A7120G) was located in exon 12. No amino acid sequence change was observed. S2 (A7261G) was located in exon 13 of intron 12, and S4 (A7337G) was located in exon 13, respectively, resulting in the mutation of Thr into Asp.. The results showed that there were three haplotypes and three diplotypes. The correlation analysis of single locus genotype and diplotype with yolk ratio, egg weight, egg yolk weight, egg shell weight and yolk color was not significant. No SNPs locus related to egg quality such as yolk ratio was found in exon 12- exon 13 of VLDLR gene of GuoShao 鈪，

本文編號：2220709