【摘要】：為了研制一種新的微生態(tài)制劑,試驗(yàn)利用分離得到的瘤胃厭氧菌Actinomyces ruminicola(命名為NY2)與痤瘡丙酸桿菌混合制成微生態(tài)制劑進(jìn)行體外發(fā)酵,研究微生態(tài)制劑的體外發(fā)酵特性及產(chǎn)揮發(fā)性脂肪酸的能力,確定微生態(tài)制劑中Actinomyces ruminicola與痤瘡丙酸桿菌的最佳混合比例。結(jié)果表明:當(dāng)微生態(tài)制劑中Actinomyces ruminicola與痤瘡丙酸桿菌混合比為1∶1時(shí),培養(yǎng)瓶中乙酸、丙酸、丁酸和總揮發(fā)性脂肪酸的濃度最高值分別達(dá)到91.07,41.12,27.58,169.19 mmol/L。該比例微生態(tài)制劑可保持瘤胃內(nèi)容物pH值在6.0左右波動(dòng),有利于瘤胃微生物發(fā)酵,并能促進(jìn)乙酸、丙酸等揮發(fā)性脂肪酸的生成。
[Abstract]:In order to develop a new microecological preparation, the isolated rumen anaerobic bacteria named NY2) was mixed with Propionibacterium Acne to produce microecological preparation for in vitro fermentation. The fermentation characteristics of microecological preparations in vitro and their ability to produce volatile fatty acids were studied. The optimum mixture ratio of Actinomyces ruminicola and Propionibacterium Acne was determined. The results showed that the highest concentration of acetic acid, propionic acid, butyric acid and total volatile fatty acids in the culture bottle was up to 91.07 mmol/L. when the mixture ratio of Actinomyces ruminicola to Propionibacterium acnes was 1:1, respectively. The pH value of rumen contents was about 6. 0, which was beneficial to the fermentation of rumen microorganism and promoted the formation of volatile fatty acids such as acetic acid and propionic acid.
【作者單位】： 黑龍江八一農(nóng)墾大學(xué)動(dòng)物科技學(xué)院;
【基金】：“十二五”國(guó)家科技支撐計(jì)劃項(xiàng)目(2013BAD21B01-2) 科技部星火計(jì)劃(2012GA670001) 黑龍江省科技廳攻關(guān)項(xiàng)目(GC05B504) 黑龍江省教育廳項(xiàng)目(1252-NCET-003) 黑龍江八一農(nóng)墾大學(xué)研究生創(chuàng)新科研項(xiàng)目(YJSCX2015-Y27)
【分類(lèi)號(hào)】：S816

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本文編號(hào)：2219571