【摘要】：本試驗旨在以相同代謝能水平為基礎(chǔ),研究高脂飼糧和高碳水化合物飼糧對大鼠脂肪代謝的影響。選取48只8周齡雄性SD大鼠,隨機(jī)分為3組,每組8個重復(fù),每個重復(fù)2只。3個組分別飼喂高脂飼糧(HF組)、高碳水化合物飼糧(HC組)和對照飼糧(CON組),試驗期9周。結(jié)果表明:1)與CON組相比,HF組、HC組大鼠的初重、末重、日增重均無顯著差異(P0.05),日采食量極顯著降低(P0.01),飼料轉(zhuǎn)化效率極顯著升高(P0.01)。2)各組大鼠的Lee’s指數(shù)、腎臟指數(shù)、胃指數(shù)、脾臟指數(shù)、肝臟指數(shù)無顯著差異(P0.05)。3)與CON組和HF組相比,HC組大鼠血清甘油三酯、總膽固醇、高密度脂蛋白膽固醇含量顯著或極顯著升高(P0.05或P0.01);與HC組和CON組相比,HF組大鼠血清葡萄糖含量極顯著增加(P0.01),血清尿素氮含量極顯著降低(P0.01)。與CON組相比,HF組和HC組大鼠肝臟甘油三酯含量極顯著升高(P0.01),HF組大鼠肝臟總膽固醇含量極顯著升高(P0.01)。4)與CON組相比,HF組和HC組大鼠肝臟磷酸烯醇式丙酮酸激酶(PEPCK)mRNA表達(dá)量極顯著增加(P0.01);與CON組和HC組相比,HF組大鼠肝臟膽固醇調(diào)節(jié)元件結(jié)合蛋白1(SREBP1)mRNA表達(dá)量極顯著增加(P0.01)。綜上所述,相同代謝能水平下,HF組和HC組大鼠體重?zé)o顯著增加。HC組大鼠血脂升高;HF組大鼠血清葡萄糖、肝臟總膽固醇含量升高,血清尿素氮含量下降。HF組和HC組大鼠肝臟甘油三酯含量升高,且通過肝臟PEPCK和SREBP1基因表達(dá)調(diào)控途徑影響脂肪代謝
[Abstract]:The aim of this study was to study the effects of high fat diet and high carbohydrate diet on fat metabolism in rats on the basis of the same metabolic energy levels. Forty-eight 8-week-old male SD rats were randomly divided into 3 groups, 8 replicates in each group and 2 in each group. The three groups were fed with high fat diet (HF group), high carbohydrate diet (HC group) and control diet (CON group) for 9 weeks. The results showed that compared with CON group, there was no significant difference in initial weight, end weight, daily gain, daily intake (P0.01), feed conversion efficiency (P0.01) and Lee's index, kidney index and gastric index of rats in HF group (P 0.05). There was no significant difference in spleen index and liver index (P0.05). Compared with CON group and HF group, serum triglyceride and total cholesterol in HC group were significantly higher than those in control group. Compared with HC group and CON group, serum glucose content was significantly increased (P0.01), serum urea nitrogen content was significantly decreased (P0.01). Compared with CON group and HC group, liver triglyceride content was significantly increased (P0.01) liver total cholesterol content in HF group was significantly higher (P0.01) .4) compared with CON group and HC group, the expression of phosphoenolpyruvate kinase (PEPCK) mRNA in liver of rats in HF group and HC group was significantly higher than that in CON group (P0.01). Compared with CON group and HC group, the expression of cholesterol regulatory element binding protein 1 (SREBP1) mRNA was significantly increased in HF group (P0.01). In conclusion, there was no significant increase in body weight of rats in HF group and HC group at the same metabolic energy level. Decrease of serum urea nitrogen content in rats liver triglyceride content was increased in HF and HC groups, and lipid metabolism was affected by regulation of PEPCK and SREBP1 gene expression in liver.
【作者單位】： 河南科技大學(xué)食品與生物工程學(xué)院;國家糧食局科學(xué)研究院;
【基金】：公益性(糧食)行業(yè)專項(201513003-8)
【分類號】：S816

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