【摘要】：為了解黃羽祖代公雞精液在禽白血病(AL)凈化中的作用,選取廣東某黃羽祖代種雞場a、b、c三個品系的公雞作為研究對象,分別無菌操作逐一采集a、b、c三個品系公雞的泄殖腔拭子及其相對應的血液與精液樣品。采用ALV p27抗原ELISA的檢測方法對泄殖腔拭子進行直接檢測,用DF-1細胞分別對血漿和精液樣品進行病毒分離,并將檢測結果進行比較分析。結果顯示:公雞的泄殖腔拭子ALV p27抗原ELISA檢測、血漿病毒分離為陰性的樣品,從其對應的公雞精液樣品中能分離到外源性ALV,并且發(fā)現(xiàn)黃羽祖代公雞精液陽性樣品及其相對應的泄殖腔拭子陽性樣品與血漿陽性樣品相互之間不能完全覆蓋;分別僅單獨檢測種公雞的泄殖腔棉拭子、精液或血液中的ALV時存在漏檢。因此,精液作為黃羽祖代公雞禽白血病凈化的檢測材料是可行的,在針對黃羽祖代公雞進行AL凈化時,不應忽略精液樣品的檢測,為了提高檢出率,加快凈化進程,應考慮不同樣品組合的復合檢測。
[Abstract]:In order to understand the role of the semen of the male cock in the purification of avian leukemia (AL), three strains of male rooster of Huangyu progeny breeding farm in Guangdong province were selected as the research object, in order to understand the role of the semen of the male cock in the purification of avian leukemia. Cloacal swabs and their corresponding blood and semen samples were collected one by aseptic operation. The cloacal swabs were directly detected by ALV p27 antigen ELISA, and the plasma and semen samples were isolated by DF-1 cells, and the results were compared and analyzed. The results showed that ALV p27 antigen ELISA was detected in cloacal swabs of rooster, and the plasma virus was isolated as negative sample. Exogenous ALV, was isolated from the corresponding rooster semen samples and it was found that the positive samples and the corresponding cloacal swabs could not be completely covered between the positive samples and plasma samples. Only the cloacal cotton swabs and ALV in semen or blood were detected separately. Therefore, it is feasible to use semen as a material for detection of avian leukemia purification in yellow feather progeny cock. In order to improve the detection rate and speed up the purification process, the detection of semen samples should not be ignored in order to improve the detection rate and speed up the purification process when purging the yellow feather progeny cock for AL purification. The composite detection of different sample combinations should be considered.
【作者單位】： 華南農業(yè)大學獸醫(yī)學院農業(yè)部獸用疫苗創(chuàng)制重點實驗室廣東省動物源性人獸共患病預防與控制重點實驗室;
【基金】：公益性行業(yè)(農業(yè))科研專項(201203055) 國家肉雞產業(yè)技術體系崗位科學家(CARS-42-G09) 廣東省科技計劃項目(2013B020224003;2015A020209145) 廣東省家禽產業(yè)技術體系(2016LM1114)
【分類號】：S855.3

【相似文獻】

相關期刊論文 前10條

1 ;蛋膜對黃羽雞的防病促長效果[J];四川畜禽;1998年01期

2 郭良星;飼料粒度對生長黃羽肉仔雞的影響[J];河南畜牧獸醫(yī);2000年08期

3 王利紅,張偉;黃羽種雞生產管理經(jīng)驗[J];中國家禽;2001年09期

4 劉志祥;;從溫氏黃羽雞育種說開去[J];中國畜禽種業(yè);2008年06期

5 ;黃羽土雞的散養(yǎng)技術[J];農村.農業(yè).農民(B版);2011年08期

6 許秀平;飼養(yǎng)“仿土型”黃羽雜交雞效益好[J];禽業(yè)科技;1997年01期

7 宋東亮,曹廣芝,龐有志,普志平,李仰義,李嘉;鵪鶉黃羽純系部分遺傳性狀觀察試驗[J];上海畜牧獸醫(yī)通訊;2003年04期

8 梁達文;優(yōu)質黃羽雞的育種[J];養(yǎng)禽與禽病防治;2004年03期

9 龐有志;宋東亮;趙淑娟;鄧雯;;隱性蛋用黃羽鵪鶉純系的建立與培育[J];中國畜禽種業(yè);2008年24期

10 申杰;杜金平;皮勁松;潘愛鑾;梁振華;蒲躍進;彭勇波;吳艷;;蛋用鵪鶉神丹黃羽系與南農黃羽系比較試驗[J];湖北畜牧獸醫(yī);2009年04期

相關會議論文 前10條

1 龐有志;吳勝軍;趙淑娟;張曉輝;于美琴;;蛋用黃羽鵪鶉微衛(wèi)星多態(tài)性分析[A];第十二次全國畜禽遺傳標記研討會論文集[C];2010年

2 吳艷;皮勁松;申杰;潘愛鑾;梁振華;蒲躍進;杜金平;;蛋用鵪鶉黃羽Ⅱ系選育進展[A];安全優(yōu)質的家禽生產——第十五次全國家禽學術討論會論文集[C];2011年

3 王麗莎;龐有志;趙淑娟;張小輝;白俊艷;，

本文編號：2204459