發(fā)布時(shí)間：2018-08-26 09:36

【摘要】：為了解黃羽祖代公雞精液在禽白血病(AL)凈化中的作用,選取廣東某黃羽祖代種雞場a、b、c三個(gè)品系的公雞作為研究對象,分別無菌操作逐一采集a、b、c三個(gè)品系公雞的泄殖腔拭子及其相對應(yīng)的血液與精液樣品。采用ALV p27抗原ELISA的檢測方法對泄殖腔拭子進(jìn)行直接檢測,用DF-1細(xì)胞分別對血漿和精液樣品進(jìn)行病毒分離,并將檢測結(jié)果進(jìn)行比較分析。結(jié)果顯示:公雞的泄殖腔拭子ALV p27抗原ELISA檢測、血漿病毒分離為陰性的樣品,從其對應(yīng)的公雞精液樣品中能分離到外源性ALV,并且發(fā)現(xiàn)黃羽祖代公雞精液陽性樣品及其相對應(yīng)的泄殖腔拭子陽性樣品與血漿陽性樣品相互之間不能完全覆蓋;分別僅單獨(dú)檢測種公雞的泄殖腔棉拭子、精液或血液中的ALV時(shí)存在漏檢。因此,精液作為黃羽祖代公雞禽白血病凈化的檢測材料是可行的,在針對黃羽祖代公雞進(jìn)行AL凈化時(shí),不應(yīng)忽略精液樣品的檢測,為了提高檢出率,加快凈化進(jìn)程,應(yīng)考慮不同樣品組合的復(fù)合檢測。
[Abstract]:In order to understand the role of the semen of the male cock in the purification of avian leukemia (AL), three strains of male rooster of Huangyu progeny breeding farm in Guangdong province were selected as the research object, in order to understand the role of the semen of the male cock in the purification of avian leukemia. Cloacal swabs and their corresponding blood and semen samples were collected one by aseptic operation. The cloacal swabs were directly detected by ALV p27 antigen ELISA, and the plasma and semen samples were isolated by DF-1 cells, and the results were compared and analyzed. The results showed that ALV p27 antigen ELISA was detected in cloacal swabs of rooster, and the plasma virus was isolated as negative sample. Exogenous ALV, was isolated from the corresponding rooster semen samples and it was found that the positive samples and the corresponding cloacal swabs could not be completely covered between the positive samples and plasma samples. Only the cloacal cotton swabs and ALV in semen or blood were detected separately. Therefore, it is feasible to use semen as a material for detection of avian leukemia purification in yellow feather progeny cock. In order to improve the detection rate and speed up the purification process, the detection of semen samples should not be ignored in order to improve the detection rate and speed up the purification process when purging the yellow feather progeny cock for AL purification. The composite detection of different sample combinations should be considered.
【作者單位】： 華南農(nóng)業(yè)大學(xué)獸醫(yī)學(xué)院農(nóng)業(yè)部獸用疫苗創(chuàng)制重點(diǎn)實(shí)驗(yàn)室廣東省動(dòng)物源性人獸共患病預(yù)防與控制重點(diǎn)實(shí)驗(yàn)室;
【基金】：公益性行業(yè)(農(nóng)業(yè))科研專項(xiàng)(201203055) 國家肉雞產(chǎn)業(yè)技術(shù)體系崗位科學(xué)家(CARS-42-G09) 廣東省科技計(jì)劃項(xiàng)目(2013B020224003;2015A020209145) 廣東省家禽產(chǎn)業(yè)技術(shù)體系(2016LM1114)
【分類號】：S855.3

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