【摘要】：為了探索湖北省黃岡市某雞場在2016年9月自然發(fā)病的大批量雞死亡的原因,為臨床診斷新城疫病毒感染提供病理學(xué)依據(jù)。采集病死雞的組織樣品,經(jīng)9日齡SPF雞胚接種傳代分離病毒后,利用HA和HI試驗(yàn)、RT-PCR擴(kuò)增F基因,對分離株進(jìn)行鑒定,并通過測定雞胚最小致死劑量的平均死亡時(shí)間、1日齡雛雞腦內(nèi)接種指數(shù)及組織病理學(xué)觀察評估分離株的毒力和致病性。結(jié)果,從送檢的病死雞中分離到1株NDV,命名為Chicken-China-Hubei0901-2016,簡稱HB0901。HB0901株F基因裂解區(qū)域氨基酸序列為112R-RQ-K-R-F117,符合強(qiáng)毒株特征。HB0901株與F17株同處于基因Ⅶ型分支上,其核苷酸序列同源性高達(dá)99.4%。該分離株的MDT和ICPI分別為47.8 h和1.96。顯微鏡下可見新城疫典型的組織病理學(xué)變化。結(jié)果表明,基因Ⅶ型新城疫毒株是導(dǎo)致該批雞大量發(fā)病死亡的原因。
[Abstract]:In order to explore the cause of death in a large number of chickens from a chicken farm in Huanggang City Hubei Province in September 2016 the pathological basis for clinical diagnosis of Newcastle disease virus infection was provided. The F gene was amplified by RT-PCR with HA and HI test after 9 days old SPF embryo inoculated to isolate the virus. The isolated strain was identified by RT-PCR. The virulence and pathogenicity of the isolated strain were evaluated by measuring the mean death time of the minimum lethal dose of chicken embryo and the inoculation index and histopathological observation of 1-day-old chicks. The results showed that one strain of NDV was isolated from the dead chicken, named Chicken-China-Hubei0901-2016. The amino acid sequence of F gene cleavage region of HB0901.HB0901 strain was 112R-RQ-K-R-F117. The nucleotide sequence homology of the nucleotide sequence was 99.4m. The MDT and ICPI of the isolate were 47.8 h and 1.96 h, respectively. The typical histopathological changes of Newcastle disease can be seen under the microscope. The results showed that the Newcastle disease virus type 鈪，

本文編號：2189944