發(fā)布時(shí)間：2018-08-15 15:39

【摘要】：胞質(zhì)內(nèi)單精子注射前對(duì)精子進(jìn)行預(yù)處理是模擬精子受精過(guò)程的變化增強(qiáng)精子解聚能力。常用的精子處理方法有聚乙烯吡咯烷酮、二硫蘇糖醇、凍融處理等。研究表明ICSI卵的卵裂率低的主要原因是精子解聚能力差。因此研究精子的處理方法提高ICSI的注射效率是十分必要的。該實(shí)驗(yàn)探討了精子不同預(yù)處理方法對(duì)ICSI胚胎發(fā)育的影響。利用超聲波細(xì)胞粉碎儀對(duì)精子做不同時(shí)間(3 s、6 s)的處理,觀(guān)察ICSI胚胎發(fā)育情況,選擇最佳的處理時(shí)間,然后利用超聲波分別處理鮮精和凍精,比較不同精液對(duì)ICSI胚胎發(fā)育的影響。將精子分別利用超聲波和堿處理,對(duì)比兩種不同預(yù)處理方法對(duì)ICSI卵卵裂率和桑囊胚率的影響,結(jié)果表明:(1)超聲波處理綿羊精子最佳的處理時(shí)間是6 s;(2)鮮精和凍精經(jīng)超聲波處理后對(duì)ICSI卵的卵裂率(39.1%vs 37.4%,P0.05)和桑囊率(12.3%vs 13.2%,P0.05)影響不顯著;(3)凍精經(jīng)堿處理后其ICSI卵的卵裂率(46.6%vs37.4%,P0.05)和桑囊胚率(20.4%vs 13.2%,P0.05)均顯著高于超聲波處理凍精。
[Abstract]:Pretreatment of spermatozoa before intracytoplasmic sperm injection is to simulate the change of sperm fertilization process to enhance the ability of sperm depolymerization. Common sperm treatment methods are polyvinylpyrrolidone, dithiothrelitol, freeze-thaw treatment and so on. The main reason for the low cleavage rate of ICSI eggs is the poor ability of sperm depolymerization. Therefore, it is necessary to study the sperm processing method to improve the injection efficiency of ICSI. The effects of different sperm pretreatment methods on the development of ICSI embryos were studied. Spermatozoa were treated with ultrasonic cell pulverizer for different time (3 s / 6 s). The development of ICSI embryos was observed and the best treatment time was selected. Then the fresh and frozen spermatozoa were treated with ultrasonic wave, respectively. The effects of different semen on ICSI embryo development were compared. The sperm were treated with ultrasound and alkali respectively. The effects of two different pretreatment methods on the cleavage rate and blastocyst rate of ICSI were compared. The results showed that: (1) the best treatment time of ultrasonic treatment was 6 s; (2) the cleavage rate of ICSI eggs (39.1%vs 37.4%) and mulberry sac rate (12.3%vs 13.2P 0.05) were not significantly affected by ultrasonic treatment of fresh and frozen spermatozoa; (3) the cleavage of ICSI eggs of frozen spermatozoa treated with alkali was not significant. The rates (46.6% vs 37.4%) and mulberry blastocyst rate (20.4%vs 13.2%) were significantly higher than that of ultrasonic treatment.
【作者單位】： 河南省農(nóng)業(yè)科學(xué)院畜牧獸醫(yī)研究所;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(2014ZX08008-003)~~
【分類(lèi)號(hào)】：S826

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