【摘要】：【目的】作為典型的荒漠動物,駱駝能夠采食其他動物不能夠食用的具有強烈氣味的或有毒的植物,而不影響其正常生理代謝。研究發(fā)現(xiàn)駱駝采食的植物毒素與吡啶、喹啉、吲哚等雜環(huán)化合物具有相似的化學結構,所以研究駱駝體內(nèi)是否存在潛在的雜環(huán)化合物降解菌具有重要意義�！痉椒ā勘狙芯坎杉�3頭駱駝瘤胃內(nèi)容物,分別以吡啶、喹啉和吲哚3種含氮雜環(huán)化合物為唯一碳源和氮源進行5代富集培養(yǎng)。通過高通量測序技術對瘤胃內(nèi)容物和5代富集培養(yǎng)細菌進行了測序分析�！窘Y果】駱駝腸道中降解雜環(huán)化合物(吡啶、喹啉、吲哚)細菌群體樣品中變形菌門、放線菌門、擬桿菌門、浮霉菌門和厚壁菌門等5個門類豐富度最高。駱駝瘤胃內(nèi)降解吡啶的優(yōu)勢菌可能屬于鞘氨醇桿菌屬和不動桿菌屬,降解吲哚的優(yōu)勢菌主要屬于芽孢桿菌屬,而降解喹啉的優(yōu)勢菌可能以賴氨酸芽孢桿菌屬和鞘氨醇桿菌屬為主�！窘Y論】駱駝瘤胃原始樣品經(jīng)過吡啶、喹啉、吲哚富集5代后,與原始樣品比較優(yōu)勢菌群發(fā)生了較大的改變,這說明駱駝瘤胃內(nèi)蘊含降解吡啶、吲哚和喹啉的細菌,但對吡啶、吲哚和喹啉的降解過程中發(fā)揮降解作用的細菌群落存在差異。
[Abstract]:[objective] as a typical desert animal, camels can feed on plants with strong odor or poison that other animals cannot eat without affecting their normal physiological metabolism. It was found that plant toxins from camels had similar chemical structures to heterocyclic compounds such as pyridine, quinoline, indole, etc. Therefore, it is of great significance to study the existence of potential heterocyclic degrading bacteria in camels. [methods] three camel rumen contents were collected and used as pyridine. Three nitrogen-containing heterocyclic compounds, quinoline and indole, were used as sole carbon and nitrogen sources for 5 generations of enrichment and culture. The rumen contents and 5 generation enriched bacteria were sequenced and analyzed by high throughput sequencing. [results] Proteus and Actinomycetes were degraded in the intestinal tract of camels (pyridine, quinoline, indole). The richness of five phylum, phylum floviformis and phylum platyphylla were the highest. The dominant bacteria for degradation of pyridine in camel's rumen may belong to sphingosine and Acinetobacter, and the dominant bacteria to degrade indole mainly belong to Bacillus. The dominant bacteria for quinoline degradation may be Lysine Bacillus and sphingosine. [conclusion] the original samples of camel rumen were enriched with pyridine, quinoline and indole for 5 generations. Compared with the original sample, the dominant flora changed greatly, which indicated that the camel rumen contains bacteria that degrade pyridine, indole and quinoline, but there are differences in the community of bacteria that play a role in the degradation of pyridine, indole and quinoline in the process of degradation of pyridine, indole and quinoline.
【作者單位】： 新疆師范大學生命科學學院新疆特殊環(huán)境物種保護與調(diào)控生物學實驗室干旱區(qū)植物逆境生物學實驗室;烏魯木齊市職業(yè)中等專業(yè)學校;成都醫(yī)學院檢驗醫(yī)學院;
【基金】：國家自然科學基金(31160028) 新疆師范大學博士科研啟動基金(XJNUBS1607) 新疆聯(lián)合基金(U1503101) 新疆維吾爾自治區(qū)自然科學基金(2016D01B049)~~
【分類號】：S824
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本文編號：2181225