甘加藏羊發(fā)情周期血漿生殖激素分泌的動態(tài)變化規(guī)律研究
發(fā)布時間：2018-08-04 13:26
【摘要】：研究表明哺乳動物下丘腦-垂體-性腺軸(Hypothalamic-pituitary-gonadal axis,HPGA)是調(diào)控動物生殖活動的重要內(nèi)分泌腺,由下丘腦、垂體和性腺分泌的生殖激素對哺乳動物的性發(fā)育、性成熟、性周期等生殖生理活動起著重要的調(diào)節(jié)作用。下丘腦分泌的促性腺激素釋放激素(GnRH)作用于腺垂體分泌促性腺激素:卵泡刺激素(Follicle stimulating hormone,FSH)和促黃體激素(Luteinizing hormone,LH),促性腺激素作用于卵巢直接參與調(diào)控母畜的發(fā)情、排卵等生殖過程。卵巢分泌雌激素和孕酮,雌激素促使雌性動物生殖器官、子宮發(fā)育和內(nèi)膜增生,刺激卵泡發(fā)育,并通過反饋調(diào)節(jié)控制下丘腦和垂體的激素分泌;孕酮可促進生殖道發(fā)育,維持家畜妊娠、保胎等生理作用。動物發(fā)情周期的不同階段中,血漿中生殖激素的濃度存在著顯著的動態(tài)變化規(guī)律。迄今為止,有關(guān)甘加藏羊發(fā)情周期生殖激素分泌的動態(tài)研究未見報道。因此,研究藏羊生殖激素分泌的動態(tài)變化規(guī)律,對揭示藏羊生殖調(diào)控機理,提高動物繁殖和生產(chǎn)性能具有重要科學(xué)意義和應(yīng)用價值。為了闡明高原甘加藏羊發(fā)情周期生殖激素分泌的動態(tài)變化在生殖活動中的作用及其與藏羊生殖調(diào)控之間的關(guān)系,本研究對甘加藏羊發(fā)情周期不同階段血漿中促卵泡素(FSH)、黃體生成素(LH)、雌激素(E)和孕酮(P)分泌的動態(tài)變化規(guī)律進行了研究。在藏羊的繁殖季節(jié)選取年齡2.5~3.5歲,發(fā)育正常的健康雌性未孕高原甘加藏羊48只,按發(fā)情周期的不同階段分為4組:發(fā)情前期、發(fā)情期、發(fā)情后期、間情期。應(yīng)用酶聯(lián)免疫吸附試驗(ELISA)測定每組藏羊發(fā)情周期各階段血漿中FSH、LH、E和P的含量,分析其在發(fā)情周期的動態(tài)變化規(guī)律。研究結(jié)果如下:1.發(fā)情周期血漿中FSH分泌特點是:在發(fā)情期分泌量最高(3.406±0.294mIU/mL),發(fā)情后期降低至(2.492±0.641 mIU/mL),間情期又開始升高,至發(fā)情前期達(2.727±0.603 mIU/mL),發(fā)情期分泌量極顯著高于發(fā)情后期、間情期和發(fā)情前期的分泌量(P0.01)。2.發(fā)情周期血漿中LH分泌特點是:在發(fā)情期、發(fā)情后期、間情期、發(fā)情前期的分泌量分別為(4.145±0.260 mIU/mL)、(3.923±0.432 m IU/mL)、(4.377±0.250 mIU/mL)、(4.375±0.466 mIU/mL),發(fā)情后期顯著低于發(fā)情期、間情期和發(fā)情前期(P0.05)。3.發(fā)情周期血漿中孕酮分泌特點是:在發(fā)情前期最高(18.039±1.596ng/mL),發(fā)情期下降為(16.196±4.183 ng/mL),發(fā)情后期分泌量緩慢升高,到間情期時分泌量達(17.127±2.857 ng/mL),發(fā)情前期顯著高于發(fā)情期與發(fā)情后期(P0.05)。4.發(fā)情周期血漿中雌激素分泌特點是:在發(fā)情前期分泌量最低(29.625±3.166 pg/mL),發(fā)情期升高(36.035±2.784 pg/mL)、發(fā)情后期分泌量達到最高(38.573±2.557 pg/mL)、間情期又降低(35.531±6.385 pg/mL),間情期分泌量極顯著高于發(fā)情前期(P0.01),發(fā)情后期極顯著高于發(fā)情期、間情期以及發(fā)情前期(P0.01),發(fā)情前期極顯著低于間情期與發(fā)情期(P0.01)。上述研究結(jié)果,可為進一步研究高原甘加藏羊生殖激素對繁殖活動的調(diào)控、對不同發(fā)育時期卵泡的作用、繁殖性能的影響,提供試驗數(shù)據(jù)。為研究哺乳動物發(fā)情、排卵等生殖內(nèi)分泌調(diào)控機理、動物繁殖育種、提高動物生產(chǎn)性能等提供試驗數(shù)據(jù)和科學(xué)理論依據(jù)。
[Abstract]:The study shows that the mammalian hypothalamus pituitary gonadal axis (Hypothalamic-pituitary-gonadal axis, HPGA) is an important endocrine gland that regulates the reproductive activities of animals. The reproductive hormones secreted by the hypothalamus, pituitary and gonadal glands play an important role in the sexual development, sexual maturity and sexual cycle of mammals. The hypothalamus is divided into the hypothalamus. Secreting gonadotropin releasing hormone (GnRH) acts on gonadotropin secreted by the adenohypophysis: Follicle stimulating hormone (FSH) and luteinizing hormone (Luteinizing hormone, LH). Gonadotropin acts on the oestrus, ovulation, and oviposition of the ovaries. The ovarian secretion is directly involved in the regulation of the oestrus and ovulation. The reproductive organs of the female animals, the development of the uterus and the hyperplasia of the endometrium, the stimulation of the follicle development, and the control of the hormone secretion in the hypothalamus and the pituitary by feedback; progesterone can promote the development of the reproductive tract, maintain the pregnancy and protect the fetus. In the different stages of the estrous cycle of the animal, the concentration of the reproductive hormone in the plasma has a significant movement. So far, the dynamic study of the secretion of reproductive hormones in the estrous cycle of Gant Tibetan sheep has not been reported. Therefore, the study of the dynamic changes in the secretion of reproductive hormones in Tibetan sheep has important scientific significance and applied value to reveal the reproductive regulation mechanism of Tibetan sheep and improve the reproduction and production performance of the animals. The relationship between the dynamic changes of the reproductive hormone secretion in the estrous cycle of sheep and the relationship between the reproductive activity and the reproductive regulation of Tibetan sheep. This study has studied the dynamic changes of the plasma levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), estrogen (E) and progesterone (P) in the different stages of the estrous cycle of Gant Tibetan sheep. 48 healthy female healthy female unpregnant Tibetan goats were divided into 4 groups according to the different stages of estrus cycle: pre estrus, estrus, anaphase, and estrus. The content of FSH, LH, E and P in each stage of estrus period of each sheep was measured by enzyme linked immunosorbent assay (ELISA), and the estrus was analyzed in the estrus. The results of the dynamic change of the cycle were as follows: 1. the secretion of FSH in the estrus cycle was: the secretion of the oestrus was highest (3.406 + 0.294mIU/mL), the late estrus decreased to (2.492 + 0.641 mIU/mL), and the estrus period began to rise, to (2.727 + 0.603 mIU/mL) in the proestrus, and the estrus secretion was significantly higher than that in the late estrus. The secretion of LH in the estrus cycle of the period and pre estrus (P0.01).2. was: the secretion of LH in estrus, anaphase, estrus, and proestrus was (4.145 + 0.260 mIU/mL), (3.923 + 0.432 m IU/mL), (4.377 + 0.250 mIU/mL), (4.375 + 0.466 mIU/mL), and significantly lower than the oestrus period, estrus and proestrus (P0.). 05) the characteristics of progesterone secretion in the.3. estrus cycle were: the highest (18.039 + 1.596ng/mL) in pre estrus, the decline of estrus (16.196 + 4.183 ng/mL), the slow increase of secretion in the late estrus, and the secretion of (17.127 + 2.857 ng/mL) at the estrous stage, which was significantly higher than the estrus and the post estrus (P0.05).4. estrus cycle in the estrus period. The secretory characteristics were: the secretion was lowest (29.625 + 3.166 pg/mL) in the pre estrus, the estrus period increased (36.035 + 2.784 pg/mL), the secretion in the late estrus reached the highest (38.573 + 2.557 pg/mL), the estrous period decreased (35.531 + 6.385 pg/mL), and the estrous period was significantly higher than that in the proestrus (P0.01), and the late estrus was significantly higher than the estrus period. The preestrus period and preestrus (P0.01) were significantly lower than that of estrus and estrus (P0.01). The above results could provide experimental data for further research on the regulation of reproductive hormones on reproductive activities in Tibetan Tibetan sheep, the effect of follicles and reproductive performance at different developmental stages, and to study the estrus and ovulation of mammals. It provides experimental data and scientific theoretical basis for regulating mechanism of reproductive endocrine, animal breeding and breeding, and improving animal production performance.
【學(xué)位授予單位】：甘肅農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S826
本文編號：2164030
[Abstract]:The study shows that the mammalian hypothalamus pituitary gonadal axis (Hypothalamic-pituitary-gonadal axis, HPGA) is an important endocrine gland that regulates the reproductive activities of animals. The reproductive hormones secreted by the hypothalamus, pituitary and gonadal glands play an important role in the sexual development, sexual maturity and sexual cycle of mammals. The hypothalamus is divided into the hypothalamus. Secreting gonadotropin releasing hormone (GnRH) acts on gonadotropin secreted by the adenohypophysis: Follicle stimulating hormone (FSH) and luteinizing hormone (Luteinizing hormone, LH). Gonadotropin acts on the oestrus, ovulation, and oviposition of the ovaries. The ovarian secretion is directly involved in the regulation of the oestrus and ovulation. The reproductive organs of the female animals, the development of the uterus and the hyperplasia of the endometrium, the stimulation of the follicle development, and the control of the hormone secretion in the hypothalamus and the pituitary by feedback; progesterone can promote the development of the reproductive tract, maintain the pregnancy and protect the fetus. In the different stages of the estrous cycle of the animal, the concentration of the reproductive hormone in the plasma has a significant movement. So far, the dynamic study of the secretion of reproductive hormones in the estrous cycle of Gant Tibetan sheep has not been reported. Therefore, the study of the dynamic changes in the secretion of reproductive hormones in Tibetan sheep has important scientific significance and applied value to reveal the reproductive regulation mechanism of Tibetan sheep and improve the reproduction and production performance of the animals. The relationship between the dynamic changes of the reproductive hormone secretion in the estrous cycle of sheep and the relationship between the reproductive activity and the reproductive regulation of Tibetan sheep. This study has studied the dynamic changes of the plasma levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), estrogen (E) and progesterone (P) in the different stages of the estrous cycle of Gant Tibetan sheep. 48 healthy female healthy female unpregnant Tibetan goats were divided into 4 groups according to the different stages of estrus cycle: pre estrus, estrus, anaphase, and estrus. The content of FSH, LH, E and P in each stage of estrus period of each sheep was measured by enzyme linked immunosorbent assay (ELISA), and the estrus was analyzed in the estrus. The results of the dynamic change of the cycle were as follows: 1. the secretion of FSH in the estrus cycle was: the secretion of the oestrus was highest (3.406 + 0.294mIU/mL), the late estrus decreased to (2.492 + 0.641 mIU/mL), and the estrus period began to rise, to (2.727 + 0.603 mIU/mL) in the proestrus, and the estrus secretion was significantly higher than that in the late estrus. The secretion of LH in the estrus cycle of the period and pre estrus (P0.01).2. was: the secretion of LH in estrus, anaphase, estrus, and proestrus was (4.145 + 0.260 mIU/mL), (3.923 + 0.432 m IU/mL), (4.377 + 0.250 mIU/mL), (4.375 + 0.466 mIU/mL), and significantly lower than the oestrus period, estrus and proestrus (P0.). 05) the characteristics of progesterone secretion in the.3. estrus cycle were: the highest (18.039 + 1.596ng/mL) in pre estrus, the decline of estrus (16.196 + 4.183 ng/mL), the slow increase of secretion in the late estrus, and the secretion of (17.127 + 2.857 ng/mL) at the estrous stage, which was significantly higher than the estrus and the post estrus (P0.05).4. estrus cycle in the estrus period. The secretory characteristics were: the secretion was lowest (29.625 + 3.166 pg/mL) in the pre estrus, the estrus period increased (36.035 + 2.784 pg/mL), the secretion in the late estrus reached the highest (38.573 + 2.557 pg/mL), the estrous period decreased (35.531 + 6.385 pg/mL), and the estrous period was significantly higher than that in the proestrus (P0.01), and the late estrus was significantly higher than the estrus period. The preestrus period and preestrus (P0.01) were significantly lower than that of estrus and estrus (P0.01). The above results could provide experimental data for further research on the regulation of reproductive hormones on reproductive activities in Tibetan Tibetan sheep, the effect of follicles and reproductive performance at different developmental stages, and to study the estrus and ovulation of mammals. It provides experimental data and scientific theoretical basis for regulating mechanism of reproductive endocrine, animal breeding and breeding, and improving animal production performance.
【學(xué)位授予單位】：甘肅農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S826
【相似文獻】
相關(guān)期刊論文 前10條
1 石成瑞;甘加羊羔的產(chǎn)肉性能試驗[J];中國畜牧雜志;1981年06期
2 石成瑞;周占榮;;甘加羊羔的育肥性能及屠宰試驗[J];甘肅畜牧獸醫(yī);1981年02期
3 楊麗;王存堂;蔣繼豐;李鵬;韓玲;余群力;;甘加藏羊屠宰性能及食用品質(zhì)研究[J];黑龍江畜牧獸醫(yī);2009年04期
4 楊勤;楊樹猛;劉漢麗;普華才讓;楊秀蘭;;甘加型藏羊選育效果淺析[J];畜牧獸醫(yī)科技信息;2008年07期
5 普華才讓;牟永娟;丁考仁青;郝恩茂;張亞林;萬瑪吉;左彩蘭;藺成友;;賈洛羊種公羊引進甘加地區(qū)適應(yīng)性觀測研究[J];畜牧獸醫(yī)雜志;2012年03期
6 農(nóng)影;;甘加藏羊 味美價高[J];致富天地;2014年01期
7 石少英;郎多勇;張玉林;;甘加型藏羊羔羊育肥試驗[J];畜牧獸醫(yī)雜志;2007年05期
8 楊樹猛;楊勤;郎多勇;劉漢麗;普華才讓;張玉林;楊秀蘭;娘代才讓;;甘加型藏羊與當?shù)孛晒叛蛲涝仔阅軐Ρ仍囼瀃J];甘肅畜牧獸醫(yī);2008年06期
9 柳小妮;蔣文蘭;劉曉靜;師尚禮;;夏河甘加草原草地蝗蟲優(yōu)勢種的確定及混合種群密度高峰值模型研究[J];草原與草坪;2007年04期
10 文欣;;盧旺達大使訪問中國農(nóng)科院蜜蜂研究所[J];中國蜂業(yè);2008年05期
相關(guān)會議論文 前2條
1 楊勤;楊樹猛;劉漢麗;普華才讓;楊秀蘭;;甘加型藏羊選育效果淺析[A];中國畜牧獸醫(yī)學(xué)會家畜生態(tài)學(xué)分會第七屆全國代表大會暨學(xué)術(shù)研討會論文集[C];2008年
2 王曉宇;;硫甘加甘露醇治療急性踝扭傷[A];第十三屆全國中西醫(yī)結(jié)合骨傷科學(xué)術(shù)研討會論文集[C];2005年
相關(guān)重要報紙文章 前2條
1 本報記者 馬玉虎 常士海;和諧甘商 魅力鄉(xiāng)鎮(zhèn)之甘加篇[N];甘南日報(漢文版);2010年
2 記者 唐亞南;沙拜次力視察夏河縣牧民定居點建設(shè)[N];甘南日報(漢文版);2007年
相關(guān)碩士學(xué)位論文 前2條
1 史軍紅;高原甘加型藏羊發(fā)情周期垂體FSH和LH細胞的表達及其激素分泌的動態(tài)變化研究[D];甘肅農(nóng)業(yè)大學(xué);2016年
2 汪倩;趙健秀《甘加丁之路》中的后現(xiàn)代主義寫作手法[D];廈門大學(xué);2014年
，本文編號：2164030
本文鏈接：http://sikaile.net/yixuelunwen/dongwuyixue/2164030.html
最近更新
教材專著
