母豬妊娠期日糧添加甲基供體或雙酚A對后代腸道發(fā)育及功能的影響
發(fā)布時間：2018-08-01 14:31
【摘要】：母體營養(yǎng)可以通過表觀遺傳修飾途徑例如改變DNA甲基化水平,持續(xù)影響后代生長發(fā)育。本試驗以能夠誘導基因發(fā)生DNA低甲基化的雙酚A (Bisphenol A,BPA)作為負對照,研究母豬妊娠期日糧添加甲基供體(Methyl donor, MET)對后代腸道發(fā)育及功能的影響,并在此基礎上考察了甲基供體是否能抵消雙酚A對腸道發(fā)育及功能的作用。選擇體重(221.14±2.57kg)、胎次(3-5)接近的60頭LY母豬,配種后隨機分為4組(n=15),妊娠期各組分別提供日糧如下：1)CON組,基礎日糧;2)BPA組,基礎日糧+雙酚A；3)MET組,基礎日糧+甲基供體；4) MET+BPA組,基礎日糧+甲基供體+雙酚A。試驗母豬從妊娠第一天開始供給相應試驗日糧,直至分娩。分娩后所有母豬自由采食同一飼糧,28天斷奶。分娩時每組選擇12頭分別來自不同母豬并且接近窩平均體重的仔豬進行屠宰取樣；斷奶時以同樣的選豬原則從每組中選擇6頭仔豬進行屠宰取樣。試驗結(jié)果如下：1、母豬妊娠期日糧添加甲基供體對新生仔豬平均體重與出生窩重無顯著影響,但顯著增加了斷奶仔豬平均體重及斷奶窩重(P0.05)。雙酚A顯著增加了新生仔豬平均體重及出生窩重(P0.05),但斷奶平均體重及斷奶窩重未受到雙酚A顯著影響。對于活產(chǎn)仔數(shù)、新生仔豬平均體重與斷奶仔豬平均體重,甲基供體與雙酚A有顯著的互作效應(P0.05)。2、母豬妊娠期日糧添加甲基供體顯著降低了新生與斷奶仔豬單位體重小腸長度(P0.05),但顯著增加了單位長度小腸重量(P0.05)。雙酚A的添加有降低新生仔豬單位體重小腸長度的趨勢(P=0.06)。3、母豬妊娠期日糧添加甲基供體顯著增加了新生和斷奶仔豬空腸與回腸的絨毛高度/隱窩深度(P0.05)。雙酚A顯著降低了新生和斷奶仔豬空腸絨毛高度/隱窩深度(P0.05)。同時,對于新生仔豬空腸和斷奶仔豬回腸絨毛高度/隱窩深度,甲基供體與雙酚A有顯著的互作效應(P0.05)。4、母豬妊娠期日糧添加甲基供體顯著增加了新生和斷奶仔豬空腸乳糖酶的活性(P0.05),同時顯著增加斷奶仔豬十二指腸和空腸蔗糖酶活性(P0.05),并且顯著上調(diào)了新生與斷奶仔豬空腸乳糖酶的基因表達(P0.05)。雙酚A顯著降低了新生仔豬空腸蔗糖酶活性(P0.05),對斷奶仔豬空腸蔗糖酶活性有降低的趨勢(P=0.07)。對于新生與斷奶仔豬空腸乳糖酶活性,甲基供體與雙酚A有顯著的互作效應(P0.05)。5、母豬妊娠期日糧添加甲基供體顯著上調(diào)了新生與斷奶仔豬空腸Pept1和Sglt1基因的相對表達量(P0.05)。雙酚A顯著下調(diào)了新生和斷奶仔豬空腸Pept1基因的相對表達量(P0.05)。同時,與BPA組相比,MET+BPA組新生與斷奶仔豬空腸Pept1基因的相對表達量顯著增加(P0.05)。6、母豬妊娠期日糧添加甲基供體顯著增加了新生仔豬空腸Pept1基因啟動子的DNA甲基化水平(P0.05),雙酚A顯著降低了新生仔豬空腸Pept1基因啟動子的DNA甲基化水平(P0.05),MET+BPA組與CON組無顯著差異。7、母豬妊娠期日糧添加甲基供體顯著增加新生仔豬空腸DNMT1、DNMT3a和MTHFR基因的相對表達量(P0.05)。雙酚A顯著降低了新生仔豬空腸DNMT3a基因的相對表達量(P0.05)。所有甲基化相關酶的基因表達在MET+BPA組與CON組均無顯著差異。綜上所述,母豬妊娠期日糧添加甲基供體能通過改善新生與斷奶仔豬腸道形態(tài),上調(diào)腸道酶活與營養(yǎng)物質(zhì)轉(zhuǎn)運載體基因表達進而提高腸道的消化吸收功能促進仔豬的生長發(fā)育。相對而言母豬妊娠期日糧添加雙酚A損傷了后代腸道發(fā)育及功能,但甲基供體能夠抵消雙酚A對腸道的損傷進而改善后代生長發(fā)育。
[Abstract]:Maternal nutrition can affect the growth and development of offspring through epigenetic modification, such as DNA methylation, which can continue to affect the growth and development of offspring. In this experiment, the diphenol A (Bisphenol A, BPA), which can induce DNA hypomethylation, was used as a negative control to study the intestinal development and function of the offspring of the sow during pregnancy (Methyl donor, MET). On this basis, we examined whether the methyl donor could counteract the effect of bisphenol A on the intestinal development and function. Select 60 LY sows with weight (221.14 + 2.57kg) and parity (3-5), and randomly divide them into 4 groups (n=15). Each group of pregnancy provides diet as follows: 1) CON, basal diet; 2) BPA, basal diet + bisphenol A; 3) M ET group, basal diet + methyl donor, 4) MET+BPA group, basal diet + methyl donor + bisphenol A. test sow from the first day of pregnancy to supply the corresponding experimental diet until delivery. After childbirth, all sows were free to feed the same diet and 28 days of weaning. Each group selected 12 of each group from different sows and close to the average weight of the nest. Pigs were slaughtered and sampled, and 6 piglets were selected from each group for slaughter sampling with the same pig selection principle during weaning. The results were as follows: 1, the average weight and litter weight of newborn piglets had no significant effect on the average weight and litter weight of newborn piglets in the sow pregnancy diet, but the average weight and weaned litter weight (P0.05) of the weaned piglets increased significantly. 1 The average weight and litter weight of newborn piglets (P0.05) were increased, but the average weaning weight and weaning litter weight were not significantly affected by bisphenol A. For the number of live births, the average weight of the newborn piglets, the average weight of the weaned piglets, the methyl donor and the bisphenol A had a significant interaction effect (P0.05).2, and the sow diet supplemented with methyl donor was significantly reduced. The unit weight small intestine length (P0.05) was lower in newborn and weanling piglets, but the small intestine weight per unit length (P0.05) was significantly increased. The addition of bisphenol A decreased the small intestine length of newborn piglets (P=0.06).3. The addition of methyl donor in sow pregnancy diet increased the height / hidden height of the jejunum and ileum in newborn and weanling piglets. Pit depth (P0.05). Bisphenol A significantly reduced the height of jejunum villus / recess depth (P0.05) in newborn and weanling piglets. At the same time, there was a significant interaction effect (P0.05) between methyl donor and bisphenol A (P0.05).4 for newborn piglet jejunum and Weanling Piglet's ileum height / recess depth. The activity of jejunum lactase (P0.05) in weanling piglets significantly increased the activity of sucrase (P0.05) in the duodenum and jejunum of weaned piglets, and significantly increased the gene expression of jejunum lactase (P0.05) in newborn and weanling piglets. Bisphenol A significantly reduced the activity of Sucrose Enzyme in newborn piglets (P0.05), and the activity of sucrase in the jejunum of weanling piglets had a significant effect on sucrase activity in weanling piglets. Decreasing trend (P=0.07). For newborn and weaned piglets, the activity of the jejunum lactase, methyl donor and bisphenol A have significant interaction effect (P0.05).5. The addition of methyl donor in sow pregnancy diet significantly up-regulated the relative apparent amount of Pept1 and Sglt1 genes between newborn and weaned piglets (P0.05). Bisphenol A significantly downgraded newborn and weanling piglets. The relative expression of the jejunum Pept1 gene (P0.05). At the same time, compared with the BPA group, the relative expression of the Pept1 gene of the jejunum in the MET+BPA group and the Weanling Piglet increased significantly (P0.05).6. The addition of methyl donor in the sow pregnancy diet significantly increased the DNA methylation level (P0.05) of the newborn piglet jejunum Pept1 gene promoter (P0.05), and the bisphenol A was significantly reduced. The DNA methylation level (P0.05) of the Pept1 gene promoter in newborn piglets was not significantly different from that in the CON group,.7. The relative expression of DNMT1, DNMT3a and MTHFR genes in newborn piglets was significantly increased by adding methyl donor in the sow gestation diet (P0.05). The relative expression of the jejunum DNMT3a gene in newborn piglets was significantly reduced. 05). The gene expression of all methylation related enzymes is not significantly different between the MET+BPA group and the CON group. To sum up, the addition of methyl donor in the sow pregnancy diet can improve the intestinal morphology of newborn and weanling piglets, up-regulation the intestinal enzyme activity and the gene expression of nutrient transport carrier and then improve the digestive and absorption function of the intestinal tract to promote the growth of piglets. Long development. Relatively speaking, the addition of bisphenol A in the diet of sow during pregnancy damage the intestinal development and function of offspring, but the methyl donor can counteract the damage of the bisphenol A to the intestine and then improve the growth and development of the offspring.
【學位授予單位】：四川農(nóng)業(yè)大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：S828.5
本文編號：2157883
[Abstract]:Maternal nutrition can affect the growth and development of offspring through epigenetic modification, such as DNA methylation, which can continue to affect the growth and development of offspring. In this experiment, the diphenol A (Bisphenol A, BPA), which can induce DNA hypomethylation, was used as a negative control to study the intestinal development and function of the offspring of the sow during pregnancy (Methyl donor, MET). On this basis, we examined whether the methyl donor could counteract the effect of bisphenol A on the intestinal development and function. Select 60 LY sows with weight (221.14 + 2.57kg) and parity (3-5), and randomly divide them into 4 groups (n=15). Each group of pregnancy provides diet as follows: 1) CON, basal diet; 2) BPA, basal diet + bisphenol A; 3) M ET group, basal diet + methyl donor, 4) MET+BPA group, basal diet + methyl donor + bisphenol A. test sow from the first day of pregnancy to supply the corresponding experimental diet until delivery. After childbirth, all sows were free to feed the same diet and 28 days of weaning. Each group selected 12 of each group from different sows and close to the average weight of the nest. Pigs were slaughtered and sampled, and 6 piglets were selected from each group for slaughter sampling with the same pig selection principle during weaning. The results were as follows: 1, the average weight and litter weight of newborn piglets had no significant effect on the average weight and litter weight of newborn piglets in the sow pregnancy diet, but the average weight and weaned litter weight (P0.05) of the weaned piglets increased significantly. 1 The average weight and litter weight of newborn piglets (P0.05) were increased, but the average weaning weight and weaning litter weight were not significantly affected by bisphenol A. For the number of live births, the average weight of the newborn piglets, the average weight of the weaned piglets, the methyl donor and the bisphenol A had a significant interaction effect (P0.05).2, and the sow diet supplemented with methyl donor was significantly reduced. The unit weight small intestine length (P0.05) was lower in newborn and weanling piglets, but the small intestine weight per unit length (P0.05) was significantly increased. The addition of bisphenol A decreased the small intestine length of newborn piglets (P=0.06).3. The addition of methyl donor in sow pregnancy diet increased the height / hidden height of the jejunum and ileum in newborn and weanling piglets. Pit depth (P0.05). Bisphenol A significantly reduced the height of jejunum villus / recess depth (P0.05) in newborn and weanling piglets. At the same time, there was a significant interaction effect (P0.05) between methyl donor and bisphenol A (P0.05).4 for newborn piglet jejunum and Weanling Piglet's ileum height / recess depth. The activity of jejunum lactase (P0.05) in weanling piglets significantly increased the activity of sucrase (P0.05) in the duodenum and jejunum of weaned piglets, and significantly increased the gene expression of jejunum lactase (P0.05) in newborn and weanling piglets. Bisphenol A significantly reduced the activity of Sucrose Enzyme in newborn piglets (P0.05), and the activity of sucrase in the jejunum of weanling piglets had a significant effect on sucrase activity in weanling piglets. Decreasing trend (P=0.07). For newborn and weaned piglets, the activity of the jejunum lactase, methyl donor and bisphenol A have significant interaction effect (P0.05).5. The addition of methyl donor in sow pregnancy diet significantly up-regulated the relative apparent amount of Pept1 and Sglt1 genes between newborn and weaned piglets (P0.05). Bisphenol A significantly downgraded newborn and weanling piglets. The relative expression of the jejunum Pept1 gene (P0.05). At the same time, compared with the BPA group, the relative expression of the Pept1 gene of the jejunum in the MET+BPA group and the Weanling Piglet increased significantly (P0.05).6. The addition of methyl donor in the sow pregnancy diet significantly increased the DNA methylation level (P0.05) of the newborn piglet jejunum Pept1 gene promoter (P0.05), and the bisphenol A was significantly reduced. The DNA methylation level (P0.05) of the Pept1 gene promoter in newborn piglets was not significantly different from that in the CON group,.7. The relative expression of DNMT1, DNMT3a and MTHFR genes in newborn piglets was significantly increased by adding methyl donor in the sow gestation diet (P0.05). The relative expression of the jejunum DNMT3a gene in newborn piglets was significantly reduced. 05). The gene expression of all methylation related enzymes is not significantly different between the MET+BPA group and the CON group. To sum up, the addition of methyl donor in the sow pregnancy diet can improve the intestinal morphology of newborn and weanling piglets, up-regulation the intestinal enzyme activity and the gene expression of nutrient transport carrier and then improve the digestive and absorption function of the intestinal tract to promote the growth of piglets. Long development. Relatively speaking, the addition of bisphenol A in the diet of sow during pregnancy damage the intestinal development and function of offspring, but the methyl donor can counteract the damage of the bisphenol A to the intestine and then improve the growth and development of the offspring.
【學位授予單位】：四川農(nóng)業(yè)大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：S828.5
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