【摘要】：為了探索雞冠發(fā)育的分子機(jī)制,利用實(shí)時(shí)熒光定量PCR技術(shù)(qRT-PCR)對前期采用轉(zhuǎn)錄組測序技術(shù)篩選出的7個(gè)與雞冠發(fā)育相關(guān)的候選基因(BMP2、TPPP3、HSD17B2、CYP2W1、CYP1A4、EDA2R、HAL)進(jìn)行表達(dá)分析。選取200只5周齡青腳麻雞A系公雞,從中篩選出15只高雞冠和15只矮雞冠公雞,采用qRT-PCR定量分析7個(gè)候選基因在兩組個(gè)體雞冠組織的相對表達(dá)量,同時(shí)檢測了7個(gè)候選基因在心、肝等11個(gè)組織的表達(dá)譜。結(jié)果顯示:CYP2W1和TPPP3在高冠組表達(dá)量極顯著高于矮冠組(P0.01);CYP4A1和EDA2R在高冠組表達(dá)量高于矮冠組(P0.05);HAL在高冠組中表達(dá)量極顯著低于矮冠組(P0.01);HSD17B2和BMP2在高冠組中表達(dá)量顯著低于矮冠組(P0.05)。表明BMP2、TPPP3、CYP2W1、EDA2R四個(gè)基因在雞冠中的表達(dá)屬于較高水平表達(dá),其余基因在雞冠組織中屬于中等水平表達(dá)。提示這7個(gè)候選基因可能在調(diào)控雞冠發(fā)育中發(fā)揮重要作用。
[Abstract]:In order to explore the molecular mechanism of the development of chicken crowns, seven candidate genes (BMP2, TPPP3, HSD17B2CYP2W1, CYP1A4, EDA2RHAL), which were screened by transcriptome sequencing technique, were analyzed by real-time fluorescence quantitative PCR (qRT-PCR). Two hundred 5-week-old A-line roosters were selected and 15 tall and 15 dwarf crowns were selected. The relative expression of 7 candidate genes in the two groups was quantitatively analyzed by qRT-PCR. At the same time, the expression profiles of 7 candidate genes in heart, liver and other 11 tissues were detected. The results showed that the expression of CYP2W1 and TPPP3 in the high crown group was significantly higher than that in the low crown group (P0.01), and the expression of CYP4A1 and EDA2R in the high crown group was significantly higher than that in the low crown group (P0.05). The expression of HSD17B2 and BMP2 in the high crown group was significantly lower than that in the low crown group (P0.05). The results showed that the expression of BMP2TPPP3CYP2W1 and EDA2R was high in the crowns, while the other genes were moderately expressed in the crowns. These 7 candidate genes may play an important role in the regulation of the development of chicken crowns.
【作者單位】： 南京農(nóng)業(yè)大學(xué);江蘇省家禽科學(xué)研究所;
【基金】：現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術(shù)體系建設(shè)專項(xiàng)資金(CARS-41) 江蘇省農(nóng)業(yè)三新工程項(xiàng)目(SXGC[2017]254) 江蘇省農(nóng)業(yè)自主創(chuàng)新資金(CX(15)1009) 江蘇省重點(diǎn)研發(fā)計(jì)劃(現(xiàn)代農(nóng)業(yè))重點(diǎn)項(xiàng)目(BE2015344) 揚(yáng)州市科技公共服務(wù)平臺建設(shè)(YZ2016221)
【分類號】：S831

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