發(fā)布時間：2018-07-29 06:20

【摘要】：【目的】分析12個微衛(wèi)星DNA與西鎮(zhèn)牛長發(fā)育性狀的關(guān)聯(lián)性,為西鎮(zhèn)牛品種資源保護和開發(fā)利用提供理論依據(jù)�！痉椒ā繙y定西鎮(zhèn)牛的體高、體長、胸圍、管圍、腰角寬和體質(zhì)量。利用12對微衛(wèi)星引物,采用PCR擴增和非變性聚丙稀酰胺凝膠電泳技術(shù),對48頭西鎮(zhèn)牛母牛進行了遺傳多樣性檢測,并統(tǒng)計分析各位點各種基因型群體與體尺性狀之間的效應(yīng)關(guān)系�！窘Y(jié)果】12個微衛(wèi)星位點各基因型與西鎮(zhèn)牛生長發(fā)育性狀具有關(guān)聯(lián)性。IDVGA2、BM2113、DVGA55、ETH225、HEL9、ILSTS005、BM1824位點與體高,BM2113、DVGA55、ETH225、HEL9、ILSTS005、ETH10、DAVG44位點與體長,IDVGA2、DVGA55、HEL9、ILST093、IDVGA27、BM1824位點與胸圍,IDVGA2、ETH225、DAVG44位點與管圍,BM2113、DVGA55、ETH225、HEL9、BM1905、ILSTS005、ILST093、ETH10位點與腰角寬,IDVGA2、BM2113、DVGA55、ETH225、HEL9、ILST093、BM1824位點與體質(zhì)量具有顯著或極顯著的效應(yīng)關(guān)系�！窘Y(jié)論】篩選出了對西鎮(zhèn)牛生長發(fā)育性狀具有正效應(yīng)的等位基因45個,具有負(fù)效應(yīng)的等位基因40個,可用于指導(dǎo)西鎮(zhèn)牛育種實踐。
[Abstract]:[objective] to analyze the correlation between 12 microsatellite DNA and the growth traits of Xizhen cattle, and to provide a theoretical basis for the conservation, exploitation and utilization of the cattle breed resources. [methods] the body height, body length, chest circumference and tube circumference of Xizhen cattle were determined. Waist width and body mass. Using 12 pairs of microsatellite primers, PCR amplification and non-denatured polyacrylamide gel electrophoresis were used to detect the genetic diversity of 48 cattle cows in Xizhen. [results] 12 microsatellite loci were correlated with the growth and development traits of Xizhen cattle. IDVGA 2, BM2113, DVGA5, ETH225, HEL9, ILSTS005, BM1824 and height BM2113DVGA5ETS005, ETH10DAVG44 and body height BM2113DVGA5ETH225, ETH10DAVG44, ETH10DAVG44, ETH10DAVG44, ETH10DAVG44, ETH10DAVG44, ETH10DAVG44, ETH10DAVG44, ETH10DAVG44, ETH10DAVG44, ETH10DAVG44 and ETH10DAVG44. ,IDVGA2,DVGA55,HEL9,ILST093,IDVGA27,BM1824浣嶇偣涓庤兏鍥，

本文編號：2151802