【摘要】：【目的】分析12個(gè)微衛(wèi)星DNA與西鎮(zhèn)牛長(zhǎng)發(fā)育性狀的關(guān)聯(lián)性,為西鎮(zhèn)牛品種資源保護(hù)和開發(fā)利用提供理論依據(jù)�！痉椒ā繙y(cè)定西鎮(zhèn)牛的體高、體長(zhǎng)、胸圍、管圍、腰角寬和體質(zhì)量。利用12對(duì)微衛(wèi)星引物,采用PCR擴(kuò)增和非變性聚丙稀酰胺凝膠電泳技術(shù),對(duì)48頭西鎮(zhèn)牛母牛進(jìn)行了遺傳多樣性檢測(cè),并統(tǒng)計(jì)分析各位點(diǎn)各種基因型群體與體尺性狀之間的效應(yīng)關(guān)系�！窘Y(jié)果】12個(gè)微衛(wèi)星位點(diǎn)各基因型與西鎮(zhèn)牛生長(zhǎng)發(fā)育性狀具有關(guān)聯(lián)性。IDVGA2、BM2113、DVGA55、ETH225、HEL9、ILSTS005、BM1824位點(diǎn)與體高,BM2113、DVGA55、ETH225、HEL9、ILSTS005、ETH10、DAVG44位點(diǎn)與體長(zhǎng),IDVGA2、DVGA55、HEL9、ILST093、IDVGA27、BM1824位點(diǎn)與胸圍,IDVGA2、ETH225、DAVG44位點(diǎn)與管圍,BM2113、DVGA55、ETH225、HEL9、BM1905、ILSTS005、ILST093、ETH10位點(diǎn)與腰角寬,IDVGA2、BM2113、DVGA55、ETH225、HEL9、ILST093、BM1824位點(diǎn)與體質(zhì)量具有顯著或極顯著的效應(yīng)關(guān)系。【結(jié)論】篩選出了對(duì)西鎮(zhèn)牛生長(zhǎng)發(fā)育性狀具有正效應(yīng)的等位基因45個(gè),具有負(fù)效應(yīng)的等位基因40個(gè),可用于指導(dǎo)西鎮(zhèn)牛育種實(shí)踐。
[Abstract]:[objective] to analyze the correlation between 12 microsatellite DNA and the growth traits of Xizhen cattle, and to provide a theoretical basis for the conservation, exploitation and utilization of the cattle breed resources. [methods] the body height, body length, chest circumference and tube circumference of Xizhen cattle were determined. Waist width and body mass. Using 12 pairs of microsatellite primers, PCR amplification and non-denatured polyacrylamide gel electrophoresis were used to detect the genetic diversity of 48 cattle cows in Xizhen. [results] 12 microsatellite loci were correlated with the growth and development traits of Xizhen cattle. IDVGA 2, BM2113, DVGA5, ETH225, HEL9, ILSTS005, BM1824 and height BM2113DVGA5ETS005, ETH10DAVG44 and body height BM2113DVGA5ETH225, ETH10DAVG44, ETH10DAVG44, ETH10DAVG44, ETH10DAVG44, ETH10DAVG44, ETH10DAVG44, ETH10DAVG44, ETH10DAVG44, ETH10DAVG44, ETH10DAVG44 and ETH10DAVG44. ,IDVGA2,DVGA55,HEL9,ILST093,IDVGA27,BM1824浣嶇偣涓庤兏鍥，

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