2株與疫苗病毒高度同源的豬繁殖與呼吸綜合征病毒的鑒定和致病性分析
發(fā)布時間：2018-07-24 20:03
【摘要】：為了解河南地區(qū)豬繁殖與呼吸綜合征病毒(PRRSV)流行毒株的特征及致病性,對從河南省兩個免疫過高致病性PRRSV(HP-PRRSV)減毒活疫苗后不久發(fā)病豬場分離的兩株P(guān)RRSV HENZK-1和HENPDS-2進行了全基因組測序、分析和致病性研究。結(jié)果表明,兩株P(guān)RRSV分離株與HP-PRRSV的細胞傳代致弱疫苗株JXA1-P80/JXA1-R高度同源。致病性試驗表明,感染HENZK-1和HENPDS-2的仔豬出現(xiàn)明顯臨床癥狀,包括高熱、食欲減退、呼吸困難等,且肺部出現(xiàn)了肉眼和組織學病變;而同源商品疫苗對照組JXA1-R雖未出現(xiàn)明顯臨床癥狀,但日增重降低,肺部有肉眼和組織學病變,并檢測、回收到病毒。綜上所述,有理由認為HENZK-1和HENPDS-2是由HP-PRRSV疫苗株JXA1-R演化而來,其來源豬群臨床發(fā)病與其毒力返強、疫苗毒株在解剖和組織學上的損傷和排毒能力有一定關(guān)系,但具體原因仍需進一步研究。本研究為謹慎使用高致病性PRRSV減毒活疫苗及PRRS控制策略提供了重要依據(jù)。
[Abstract]:In order to understand the characteristics and pathogenicity of the porcine reproductive and respiratory syndrome virus (PRRSV) virus (PRRSV) strains, two PRRSV HENZK-1 and HENPDS-2 isolated from two highly pathogenic PRRSV (HP-PRRSV) live attenuated vaccines in Henan province were sequenced, analyzed and pathogenicity. The results showed that two strains were found. The PRRSV isolates were highly homologous to the HP-PRRSV cell line induced weak vaccine strain JXA1-P80/JXA1-R. The pathogenicity test showed that the piglets infected with HENZK-1 and HENPDS-2 had obvious clinical symptoms, including high fever, anorexia, and dyspnea, and the lung appeared naked eye and histologic changes, while JXA1-R in the homologous vaccine control group did not appear to be clear. The clinical symptoms, but the decrease of the daily weight, the lung have the naked eye and the histology, and the detection and recovery of the virus. In summary, there is a reason to think that HENZK-1 and HENPDS-2 are evolved from the HP-PRRSV vaccine strain JXA1-R, and that the disease of the pigs and the virulence of the pigs are strong, and the damage and detoxification ability of the vaccine strain in the anatomy and histology and the detoxification ability are certain. However, the specific reasons still need further study. This study provides an important basis for prudent use of highly pathogenic PRRSV attenuated live vaccine and PRRS control strategy.
【作者單位】： 河南農(nóng)業(yè)大學牧醫(yī)工程學院;中國獸醫(yī)藥品監(jiān)察所;
【基金】：國家重點研發(fā)計劃(項目號:2016YFD0501102),題目:潛在入侵的畜禽疫病檢測鑒定新技術(shù)研究 河南省高�？萍紕�(chuàng)新團隊與支持計劃資助項目(項目號:14IRTSHN015),題目:動物疫病防控新技術(shù)~~
【分類號】：S852.65
本文編號：2142503
[Abstract]:In order to understand the characteristics and pathogenicity of the porcine reproductive and respiratory syndrome virus (PRRSV) virus (PRRSV) strains, two PRRSV HENZK-1 and HENPDS-2 isolated from two highly pathogenic PRRSV (HP-PRRSV) live attenuated vaccines in Henan province were sequenced, analyzed and pathogenicity. The results showed that two strains were found. The PRRSV isolates were highly homologous to the HP-PRRSV cell line induced weak vaccine strain JXA1-P80/JXA1-R. The pathogenicity test showed that the piglets infected with HENZK-1 and HENPDS-2 had obvious clinical symptoms, including high fever, anorexia, and dyspnea, and the lung appeared naked eye and histologic changes, while JXA1-R in the homologous vaccine control group did not appear to be clear. The clinical symptoms, but the decrease of the daily weight, the lung have the naked eye and the histology, and the detection and recovery of the virus. In summary, there is a reason to think that HENZK-1 and HENPDS-2 are evolved from the HP-PRRSV vaccine strain JXA1-R, and that the disease of the pigs and the virulence of the pigs are strong, and the damage and detoxification ability of the vaccine strain in the anatomy and histology and the detoxification ability are certain. However, the specific reasons still need further study. This study provides an important basis for prudent use of highly pathogenic PRRSV attenuated live vaccine and PRRS control strategy.
【作者單位】： 河南農(nóng)業(yè)大學牧醫(yī)工程學院;中國獸醫(yī)藥品監(jiān)察所;
【基金】：國家重點研發(fā)計劃(項目號:2016YFD0501102),題目:潛在入侵的畜禽疫病檢測鑒定新技術(shù)研究 河南省高�？萍紕�(chuàng)新團隊與支持計劃資助項目(項目號:14IRTSHN015),題目:動物疫病防控新技術(shù)~~
【分類號】：S852.65
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