1例雞心包積水-肝炎綜合征的實(shí)驗(yàn)室診斷
[Abstract]:In September 2015, an infectious disease occurred in broilers raised at a farm in Jiangsu Province. The diseased chickens were characterized by depression, short incubation period and high mortality, and the pericardial effusion and liver were found to be yellowish and haemorrhage. The liver and pericardial effusion of diseased chickens were collected and isolated by wheat Kang Kai Agar plate and blood plate respectively. The results were negative. According to the Hexon gene of inclusion body hepatitis (IBH), primers were designed to detect the pathogen. The results showed that using DNA extracted from liver and pericardial effusion of diseased chicken as template, about 500bp IBH specific bands could be amplified by PCR. FAd V-4 adenovirus was identified by sequencing and analysis. The disease was diagnosed as a result of FAd V-4 adenovirus infection through laboratory diagnosis combined with clinical symptoms and epidemiology.
【作者單位】: 江蘇省邳州市官湖鎮(zhèn)畜牧獸醫(yī)站;江蘇省新沂踢球山種畜場;
【分類號(hào)】:S858.31
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