【摘要】：為了觀察甘草黃酮對(duì)氧化損傷小鼠腦、肝臟、肺臟組織形態(tài)的影響,試驗(yàn)把60只小鼠隨機(jī)分成6組,每組10只,即空白對(duì)照組、模型組、維生素C組、甘草黃酮低劑量組、甘草黃酮中劑量組、甘草黃酮高劑量組,除空白對(duì)照組外其余各組連續(xù)采用甘草黃酮灌胃小鼠42 d,再灌胃乙醇建立乙醇損傷模型,觀察不同濃度的甘草黃酮(40,100,150 mg/kg)灌服小鼠后試驗(yàn)現(xiàn)象,并處死小鼠后快速取出全腦、肝臟、肺臟進(jìn)行H.E.染色,觀察組織形態(tài)。結(jié)果表明:模型組小鼠腦梗死區(qū)域可見大量神經(jīng)元變性、壞死,胞體縮小變形,核固縮,噬神經(jīng)元現(xiàn)象,腦組織水腫。與模型組相比,甘草黃酮低劑量組小鼠病理改變的嚴(yán)重程度降低,但可見小量神經(jīng)元變性壞死;甘草黃酮中劑量組小鼠病理改變較模型組輕,神經(jīng)元變性壞死部分的數(shù)量減少,噬神經(jīng)元現(xiàn)象也明顯降低,腦組織水腫明顯降低;甘草黃酮高劑量組小鼠梗死區(qū)亦可見少量的神經(jīng)元變性、壞死,見不到"噬神經(jīng)元現(xiàn)象",腦組織無水腫。甘草黃酮3個(gè)劑量組與乙醇氧化損傷模型相比較組織形態(tài)明顯有差異。說明甘草黃酮對(duì)小鼠腦、肝臟、肺臟組織病理學(xué)改變具有明顯的改善作用,且都呈現(xiàn)明顯的劑量依賴效應(yīng)。
[Abstract]:In order to observe the effects of Glycyrrhiza flavonoids on the brain, liver and lung tissue of mice with oxidative damage, 60 mice were randomly divided into 6 groups, with 10 rats in each group, namely, blank control group, model group, vitamin C group, low dose group of Glycyrrhiza flavonoids, dose group of Glycyrrhiza flavone, high dose of Glycyrrhiza flavone, and other groups in addition to blank control group. The flavonoid was perfused to 42 d in mice, then ethanol was perfused to establish a model of ethanol damage, and the effects of different concentrations of glycyrrhizin (40100150 mg/kg) on the mice were observed. After the mice were killed, the whole brain, liver and lungs were taken out by H.E. staining, and the tissue morphology was observed. The results showed that a large number of neurons in the cerebral infarction area of the model group could be seen in a large number of neuron changes. Compared with the model group, the severity of the pathological changes in the low dose group of Glycyrrhiza flavonoids decreased, but the small amount of neurons were degenerative and necrotic, and the dose group of Glycyrrhiza flavanone was lighter than the model group, and the number of neuron degeneration and necrosis was reduced. The neuron phenomenon was obviously decreased and the edema of brain tissue decreased obviously, and a small amount of neuron degeneration and necrosis were found in the high dose group of Glycyrrhiza flavanone group, and there was no edema in the brain tissue. The 3 dose groups of Glycyrrhiza flavonoids were significantly different from the ethanol oxidative damage model. Histopathological changes in brain, liver and lungs of mice were significantly improved, and both showed a dose-dependent effect.
【作者單位】： 新疆生產(chǎn)建設(shè)兵團(tuán)塔里木畜牧科技重點(diǎn)實(shí)驗(yàn)室;塔里木大學(xué)動(dòng)物科學(xué)學(xué)院;
【基金】：大學(xué)生創(chuàng)新創(chuàng)業(yè)訓(xùn)練計(jì)劃項(xiàng)目(2014029)
【分類號(hào)】：S853.7

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