培育方式對(duì)羔羊生長(zhǎng)發(fā)育和肝臟基因表達(dá)的影響
發(fā)布時(shí)間：2018-07-18 15:25
【摘要】：本文以雙胞胎羔羊?yàn)樵囼?yàn)動(dòng)物研究培育方式對(duì)羔羊生長(zhǎng)發(fā)育和相關(guān)基因表達(dá)的影響。選取24對(duì)雙胞胎湖羊公羔羊,采用配對(duì)試驗(yàn)設(shè)計(jì),將每對(duì)雙胞胎羔羊分成兩組,一組為試驗(yàn)組,實(shí)行人工哺育代乳品(AR,artificially reared);另一組為對(duì)照組,進(jìn)行隨母哺乳(ER,ewe reared),每組24只羔羊。羔羊出生后,每隔10天于晨飼前進(jìn)行稱(chēng)重、采血。羔羊在45~55日齡和75~85日齡隨機(jī)選取4對(duì)雙胞胎羔羊采用全收糞尿法進(jìn)行消化代謝試驗(yàn)。羔羊在60和90日齡時(shí),分別隨機(jī)選取3對(duì)雙胞胎羔羊進(jìn)行屠宰。采用基因芯片技術(shù)和生物信息學(xué)的方法分析肝臟組織的差異表達(dá)基因。試驗(yàn)一,培育方式對(duì)雙胞胎湖羊羔羊生長(zhǎng)發(fā)育、營(yíng)養(yǎng)物質(zhì)利用率和血清學(xué)指標(biāo)的影響。結(jié)果顯示,在20、30、40日齡時(shí),AR組羔羊的體重顯著低于ER組(P0.05),但差異在逐漸減小,到50日齡以后差異不顯著(P0.05)。AR組羔羊的平均日增重在10~20日齡段顯著低于ER組(P0.05),在40~50日齡段顯著高于ER組(P0.05),其他時(shí)段差異不顯著,全期的平均日增重差異亦不顯著(P0.05)。對(duì)消化器官發(fā)育的影響:在60日齡時(shí),AR組羔羊的瘤胃占復(fù)胃總重的比例顯著高于ER組(P0.05),而瓣胃和皺胃占復(fù)胃總重的比例顯著低于ER組(P0.05);在90日齡時(shí),兩組羔羊各胃室鮮重以及占復(fù)胃總重的比例均無(wú)顯著性差異(P0.05),但AR組羔羊瘤胃的鮮重比ER組高。AR組羔羊的小腸占宰前活重的比例顯著高于ER組(P0.05)。代乳品對(duì)羔羊內(nèi)臟器官重量的影響差異不顯著(P0.05);對(duì)干物質(zhì)(DM)、有機(jī)物(OM)、能量(E)、粗蛋白(CP)、粗脂肪(EE)、鈣(Ca)、磷(P)等營(yíng)養(yǎng)物質(zhì)的表觀消化率均無(wú)顯著影響(P0.05);對(duì)總蛋白(TP)、白蛋白(ALB)、球蛋白(GLOB)、血中尿素氮(BUN)、膽固醇(CHOL)、甘油三酯(TG)、葡萄糖(GLU)、堿性磷酸酶(ALP)等血清生化指標(biāo)以及Ig G、Ig M、Ig A等血清免疫指標(biāo)的影響不明顯(P0.05),AR組與ER組羔羊在不同時(shí)期的血清學(xué)指標(biāo)呈現(xiàn)出相似的變化規(guī)律。試驗(yàn)二,培育方式對(duì)雙胞胎湖羊羔羊肝臟基因表達(dá)的影響。通過(guò)基因芯片分析發(fā)現(xiàn)了因培育方式不同引起的差異表達(dá)基因。AR組和ER組的差異表達(dá)基因在60日齡和90日齡都發(fā)生改變的基因有7個(gè),它們是OR1E2、S1PR3、KMO、APOA4、ORM2、HTR4和FADS1;60日齡和90日齡的差異表達(dá)基因在AR組和ER組中表達(dá)也發(fā)生變化的基因有20個(gè),它們是ACO1、HBA1、MFSD2A、CYSJ、LOC785954、FCN、GRB2、LOC100037663、ORM2、RXRG、LOC100602447、SQLE、HSD17B2、CYP4F2、INHBE、EAAT2,其中有4個(gè)未知基因,探針號(hào)為14780690、14815720、14845504、14854080;在不同日齡和不同處理中,表達(dá)均發(fā)生改變的基因是ORM2。GO功能顯著性富集分析發(fā)現(xiàn),這些差異表達(dá)基因主要參與氨基酸、小分子化合物和有機(jī)酸代謝的生物過(guò)程、酶活性反應(yīng)生物過(guò)程和免疫反應(yīng)過(guò)程。Pathway途徑顯著性分析發(fā)現(xiàn),這些差異表達(dá)基因主要參與氨基酸代謝、脂類(lèi)代謝、激素合成和p53信號(hào)轉(zhuǎn)導(dǎo)途徑�？傮w結(jié)論,人工飼喂代乳粉的培育方式能夠達(dá)到與母乳哺育羔羊相同的培育效果。不同的培育方式導(dǎo)致肝臟的基因表達(dá)發(fā)生了改變,發(fā)現(xiàn)了肝臟的差異表達(dá)基因。通過(guò)這些差異表達(dá)基因所參與的生物學(xué)過(guò)程及其發(fā)揮的生物學(xué)功能,初步探討了羔羊早期生長(zhǎng)發(fā)育的營(yíng)養(yǎng)調(diào)控作用,為揭示羔羊早期培育的分子機(jī)理奠定了基礎(chǔ)。
[Abstract]:The effects of the twin lambs on the growth and gene expression of lambs were studied in this study. 24 pairs of twin lambs were selected to divide the twin lambs into two groups, one group was the experimental group and the AR (artificially reared) was carried out. The other group was the control group. ER, ewe reared, each group of 24 lambs. After birth, the lambs were weighed and collected every 10 days before the morning feeding. The lambs randomly selected 4 pairs of twin lambs at 45~55 days and 75~85 days to carry out the digestion and metabolism test by full dung urine. The lamb was slaughtered at 60 and 90 days of age. The gene chip technology and bioinformatics method were used to analyze the differential expression genes of liver tissue. Experiment 1, the effects of cultivation methods on the growth and development, nutrient utilization ratio and serological index of the twin lambs were studied. The results showed that the weight of the AR lambs was significantly lower than that of the ER group (P0.05) at the age of 20,30,40, but the difference was gradually reduced. The average daily weight gain of lambs in group.AR was significantly lower than that of group ER (P0.05) in 10~20 days of age after 50 days of age (P0.05), and at the age of 40~50 was significantly higher than that in group ER (P0.05). The difference in other periods was not significant, and the difference in average daily weight gain was not significant (P0.05). The effects on the development of digestive organs: at the age of 60 days, the tumor of AR group lambs The proportion of the stomach to the total weight of the stomach was significantly higher than that in the ER group (P0.05), while the proportion of the omasum and the abomasum to the total weight of the stomach was significantly lower than that of the ER group (P0.05). At 90 days of age, there was no significant difference in the fresh weight of the gastric chamber and the total weight of the stomach in the two groups of lambs (P0.05), but the fresh weight of the rumen in the AR group was higher than that of the ER group and the small intestine of the group of the.AR lambs occupied the live weight before the slaughter. The difference was significantly higher than that in the ER group (P0.05). There was no significant difference in the weight of the visceral organs of the Lambs (P0.05); the apparent digestibility of the nutrients (DM), organic matter (OM), energy (E), crude protein (CP), crude fat (EE), calcium (Ca), phosphorus (P) and other nutrients were not significantly affected (TP), albumin, globulin, and blood urine. Serum biochemical indexes such as BUN, cholesterol (CHOL), triglyceride (TG), glucose (GLU), alkaline phosphatase (ALP), and serum Ig G, Ig M, Ig A and other serum immune indexes were not obvious (P0.05). By gene chip analysis, we found that the differentially expressed genes in.AR and ER groups of different expression genes,.AR and ER, were changed at 60 days and 90 days of age. They were OR1E2, S1PR3, KMO, APOA4, ORM2, HTR4 and FADS1, and the differentially expressed genes of 60 days and 90 days of age were in AR group and E. There are 20 genes that also change in group R, they are ACO1, HBA1, MFSD2A, CYSJ, LOC785954, FCN, GRB2, LOC100037663, ORM2, RXRG, there are 4 unknown genes. Because of the significant enrichment analysis of ORM2.GO function, these differentially expressed genes are mainly involved in amino acids, small molecular compounds and biological processes of organic acid metabolism, enzyme activity biological processes and.Pathway pathway significance analysis of immunoreaction process. These differentially expressed genes are mainly involved in amino acid metabolism, lipid metabolism, and excitation. Synthetic and p53 signal transduction pathways. Overall conclusion, artificial feeding of milk powder can achieve the same cultivation effect as breastfeeding lamb. Different breeding methods lead to the changes in gene expression in the liver and the differential expression genes of the liver. Its biological function has preliminarily discussed the nutritional regulation function of lamb's early growth and development, and laid a foundation for revealing the molecular mechanism of early lamb breeding.
【學(xué)位授予單位】：中國(guó)農(nóng)業(yè)科學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類(lèi)號(hào)】：S826
本文編號(hào)：2132330
[Abstract]:The effects of the twin lambs on the growth and gene expression of lambs were studied in this study. 24 pairs of twin lambs were selected to divide the twin lambs into two groups, one group was the experimental group and the AR (artificially reared) was carried out. The other group was the control group. ER, ewe reared, each group of 24 lambs. After birth, the lambs were weighed and collected every 10 days before the morning feeding. The lambs randomly selected 4 pairs of twin lambs at 45~55 days and 75~85 days to carry out the digestion and metabolism test by full dung urine. The lamb was slaughtered at 60 and 90 days of age. The gene chip technology and bioinformatics method were used to analyze the differential expression genes of liver tissue. Experiment 1, the effects of cultivation methods on the growth and development, nutrient utilization ratio and serological index of the twin lambs were studied. The results showed that the weight of the AR lambs was significantly lower than that of the ER group (P0.05) at the age of 20,30,40, but the difference was gradually reduced. The average daily weight gain of lambs in group.AR was significantly lower than that of group ER (P0.05) in 10~20 days of age after 50 days of age (P0.05), and at the age of 40~50 was significantly higher than that in group ER (P0.05). The difference in other periods was not significant, and the difference in average daily weight gain was not significant (P0.05). The effects on the development of digestive organs: at the age of 60 days, the tumor of AR group lambs The proportion of the stomach to the total weight of the stomach was significantly higher than that in the ER group (P0.05), while the proportion of the omasum and the abomasum to the total weight of the stomach was significantly lower than that of the ER group (P0.05). At 90 days of age, there was no significant difference in the fresh weight of the gastric chamber and the total weight of the stomach in the two groups of lambs (P0.05), but the fresh weight of the rumen in the AR group was higher than that of the ER group and the small intestine of the group of the.AR lambs occupied the live weight before the slaughter. The difference was significantly higher than that in the ER group (P0.05). There was no significant difference in the weight of the visceral organs of the Lambs (P0.05); the apparent digestibility of the nutrients (DM), organic matter (OM), energy (E), crude protein (CP), crude fat (EE), calcium (Ca), phosphorus (P) and other nutrients were not significantly affected (TP), albumin, globulin, and blood urine. Serum biochemical indexes such as BUN, cholesterol (CHOL), triglyceride (TG), glucose (GLU), alkaline phosphatase (ALP), and serum Ig G, Ig M, Ig A and other serum immune indexes were not obvious (P0.05). By gene chip analysis, we found that the differentially expressed genes in.AR and ER groups of different expression genes,.AR and ER, were changed at 60 days and 90 days of age. They were OR1E2, S1PR3, KMO, APOA4, ORM2, HTR4 and FADS1, and the differentially expressed genes of 60 days and 90 days of age were in AR group and E. There are 20 genes that also change in group R, they are ACO1, HBA1, MFSD2A, CYSJ, LOC785954, FCN, GRB2, LOC100037663, ORM2, RXRG, there are 4 unknown genes. Because of the significant enrichment analysis of ORM2.GO function, these differentially expressed genes are mainly involved in amino acids, small molecular compounds and biological processes of organic acid metabolism, enzyme activity biological processes and.Pathway pathway significance analysis of immunoreaction process. These differentially expressed genes are mainly involved in amino acid metabolism, lipid metabolism, and excitation. Synthetic and p53 signal transduction pathways. Overall conclusion, artificial feeding of milk powder can achieve the same cultivation effect as breastfeeding lamb. Different breeding methods lead to the changes in gene expression in the liver and the differential expression genes of the liver. Its biological function has preliminarily discussed the nutritional regulation function of lamb's early growth and development, and laid a foundation for revealing the molecular mechanism of early lamb breeding.
【學(xué)位授予單位】：中國(guó)農(nóng)業(yè)科學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類(lèi)號(hào)】：S826
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，本文編號(hào)：2132330
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