發(fā)布時間：2018-07-17 08:23

【摘要】：本文研究了不同添加劑量的復(fù)方中藥“豬康散”預(yù)防用藥對人工感染PRRSV豬血清和組織抗氧化功能的影響,旨在闡明復(fù)方中藥“豬康散”對豬繁殖與呼吸綜合征的預(yù)防效果,為其在養(yǎng)豬業(yè)應(yīng)用推廣提供科學(xué)依據(jù)。試驗選取經(jīng)檢測臨床健康、體重相近、PRRSV抗原為陰性的35日齡杜長大三元雜交仔豬40頭,隨機分為4組,每組10頭。41-44日齡時中藥組(Ⅲ、Ⅳ組)分別在基礎(chǔ)日糧中添加0.5%、1.0%“豬康散”,41日齡時除陰性對照組(Ⅰ組)外均滴鼻接種PRRSVSCM株1.0mL。于40、45、50、55、60日齡時,四個組分別隨機選取4頭仔豬前腔靜脈無菌采血,分離血清用于檢測抗氧化指標(biāo),并在仔豬50、60日齡每組隨機選取4頭采集肝臟、肺臟、脾臟和腎臟組織,制備組織勻漿,測定組織抗氧化指標(biāo)。試驗結(jié)果表明：(1)整個試驗期間,與陰性和陽性對照組相比,除40d外,0.5%、1.0%“豬康散”預(yù)防用藥均能顯著提高人工感染PRRSV仔豬血清中SOD活性(P0.01或0.05)；除肝臟和50d肺臟外,1.0%中藥預(yù)防組各組織中SOD活性均極顯著高于兩對照組(P0.01)；60d時,0.5%中藥預(yù)防組肝臟、脾臟和腎臟中SOD活性均極顯著高于陽性對照組(P0.01)。(2)與陰性和陽性對照組相比,除40d、0.5%中藥預(yù)防組第45d、60d外,0.5%、1.0%“豬康散”預(yù)防用藥均能極顯著提高人工感染PRRSV仔豬血清中GSH-Px活性(P0.01)；整個試驗期間,除0.5%中藥預(yù)防組肺和50d脾外,0.5%、1.0%中藥預(yù)防組仔豬各組織中GSH-Px活力均極顯著高于兩對照組(P0.01)。(3)與陰性和陽性對照組相比,除40d、0.5%中藥預(yù)防組第45d外,0.5%、1.0%“豬康散”預(yù)防用藥均能極顯著提高人工感染PRRSV仔豬血清中CAT活性(P0.01)；除陰性對照組60d肺臟外,1.0%中藥預(yù)防組各組織中CAT活性均顯著高于兩對照組(P0.01或0.05),除50d肺,0.5%中藥預(yù)防組各組織中CAT活力均極顯著高于陽性對照組(P0.01)。(4)與陰性和陽性對照組相比,除40d和0.5%中藥預(yù)防組第45d外,0.5%、1.0%“豬康散”預(yù)防用藥均能極顯著提高人工感染PRRSV仔豬血清中T-AOC能力(P0.01)；1.0%中藥預(yù)防組各組織中T-AOC能力均極顯著高于兩對照組(P0.01),0.5%中藥預(yù)防組各組織中T-AOC能力均極顯著高于陽性對照組(P0.01)。(5)與陰性和陽性對照組相比,除40d和0.5%中藥預(yù)防組第45d外,0.5%、1.0%“豬康散”預(yù)防用藥均能極顯著降低人工感染PRRSV仔豬血清MDA含量(P0.01)；除陰性對照組腎、肺臟和60d脾臟外,1.0%中藥預(yù)防組各組織MDA含量均極顯著低于兩對照組(P0.01),0.5%中藥預(yù)防組各組織MDA含量顯著低于陽性對照組(P0.01或0.05)。(6)整個試驗期間,與陰性和陽性對照組相比,除40d外,0.5%、1.0%“豬康散”預(yù)防用藥均能極顯著提高人工感染PRRSV仔豬血清中抑制·OH能力(P0.01)；除陰性對照組50d肝和50d腎臟外,0.5%、1.0%中藥預(yù)防組仔豬各組織中抑制OH·能力均顯著高于兩對照組(P0.01或0.05)。上述結(jié)果表明：0.5%和1.0%復(fù)方中藥“豬康散”預(yù)防用藥能明顯提高人工感染PRRSV仔豬血清和組織SOD、GSH-Px、CAT、T-AOC活力和抑制·OH能力,降低MDA含量的作用,從而提高感染PRRSV仔豬血清和組織抗氧化功能,達到清除自由基、改善機能,提高抗PRRSV的能力,并以1.0%中藥添加劑量更佳。
[Abstract]:The effect of the compound Chinese medicine "pig Kang San" on the serum and tissue antioxidant function of PRRSV pigs with artificial infection was studied in this paper. The purpose of this study was to clarify the preventive effect of "pig Kang San" on porcine reproductive and respiratory syndrome, and to provide scientific basis for its application in pig breeding. Healthy, body weight similar, PRRSV antigen negative 35 days old three yuan hybrid piglets were randomly divided into 4 groups, each group of 10.41-44 days of age (Group III, IV) added 0.5%, 1% "pig Kang San" in basal diet, and 41 days old, except for negative control group (group I), inoculated PRRSVSCM strain 1.0mL. at 40,45,50,55,60 days of age In the four groups, the four groups were randomly selected for the aseptic blood sampling of the anterior vena cava of 4 piglets, and the isolated serum was used to detect the antioxidative index. At the age of 50,60 day of the piglets, 4 heads of each group were randomly selected to collect liver, lung, spleen and kidney tissue. The tissue homogenate was prepared and the tissue antioxidant indexes were prepared. The results showed that (1) during the whole experiment, it was negative and positive. Compared with the sex control group, 0.5% and 1% "pigs Kang San" could significantly increase the activity of SOD in the serum of PRRSV piglets with artificial infection (P0.01 or 0.05). Except for the liver and 50D lung, the activity of SOD in each tissue of the prevention group of Chinese medicine was significantly higher than that of the two control group (P0.01), and 0.5% of the liver, spleen and kidneys of the traditional Chinese medicine group were in 60d. The activity of D was significantly higher than that of the positive control group (P0.01). (2) compared with the negative and positive control group, the serum GSH-Px activity (P0.01) of the artificially infected PRRSV piglets was significantly improved, except for 40d, 0.5%, 60d, 0.5%, 1%, and 1% "pig Kang San", except 0.5% Chinese medicine prevention group lung and 50D spleen, 0.5%, 1 during the whole test. The activity of GSH-Px in.0% prevention group was significantly higher than that of the two control group (P0.01). (3) compared with the negative and positive control group, except 40d, 0.5%, 0.5% and 1% "pig Kang San" could significantly improve the CAT activity (P0.01) in the serum of PRRSV piglets, except for the negative control group 60d lung, 1 .0% the activity of CAT in each group of Chinese medicine prevention group was significantly higher than that of the two control group (P0.01 or 0.05). Except for 50D lung, the activity of CAT was significantly higher than that of the positive control group (P0.01). (4) compared with the negative and positive control group, the prevention of the 0.5% and 1% "pig Kang San" was extremely significant except for 40d and 0.5% of the prevention group 45d. Increase the T-AOC ability (P0.01) in the serum of PRRSV piglets with artificial infection. 1% the ability of T-AOC in each group of Chinese medicine prevention group was significantly higher than that of the two control group (P0.01). 0.5% the T-AOC ability in each group of Chinese medicine prevention group was significantly higher than that of the positive control group (P0.01). (5) compared with the negative and positive control group, except 40d and 0.5% traditional Chinese medicine prevention group 45d, 0. 5%, 1% "pig Kang San" can significantly reduce the serum MDA content of PRRSV piglets (P0.01). Except for the kidney, lung and 60d spleen in the negative control group, the content of MDA in each tissue of 1% Chinese medicine prevention group is significantly lower than that of the two control group (P0.01). 0.5% the content of MDA in each group of traditional Chinese medicine prevention group is significantly lower than that of the positive control group (P0.01 or 0.05) (6) during the whole test period, compared with the negative and positive control group, except 40d, 0.5% and 1% "pig Kang San" can significantly improve the inhibitory and OH ability (P0.01) in the serum of artificial infection PRRSV piglets, and the inhibition of OH in each tissue of the piglets of the 50D liver and 50D kidney in the negative control group was significantly higher than that of the two. The control group (P0.01 or 0.05). The results showed that the prophylactic use of "pig Kang San" in compound Chinese medicine of 0.5% and 1% can obviously improve the serum and tissue SOD, GSH-Px, CAT, T-AOC activity and the ability to inhibit the OH, reduce the function of the MDA content, so as to improve the antioxidant function of serum and tissue in the infected PRRSV piglets and achieve the clearance of freedom. It can improve the function and improve the ability of resisting PRRSV, and the dosage of 1% Chinese medicine is better.
【學(xué)位授予單位】：四川農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S858.28

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