日糧對(duì)細(xì)毛羊瘤胃上皮的SCFA吸收相關(guān)基因及氮素轉(zhuǎn)運(yùn)的調(diào)控
發(fā)布時(shí)間：2018-07-13 08:35
【摘要】：本文通過(guò)飼養(yǎng)試驗(yàn)、熒光定量和Western blot方法探求日糧不同的NFC／NDF與蛋白水平對(duì)內(nèi)蒙古細(xì)毛羊瘤胃上皮VFA吸收相關(guān)基因表達(dá)量及氮素轉(zhuǎn)運(yùn)的調(diào)控。本論文的研究?jī)?nèi)容和結(jié)果具體如下：試驗(yàn)1日糧不同NFC/NDF對(duì)細(xì)毛羊消化代謝、瘤胃內(nèi)環(huán)境及SCFA吸收的影響選用9只體況良好,體重為43.8±4.75Kg有永久性瘤胃瘺管的內(nèi)蒙古鄂爾多斯細(xì)毛羊半同胞羯羊,按體重分為3組：NFC/NDF分別為：0.35、0.66和1.14,蛋白水平相同,為13.5%。飼養(yǎng)試驗(yàn)表明日糧NFC/NDF對(duì)細(xì)毛羊氮代謝無(wú)顯著影響。糞中的NDF隨日糧NFC/NDF的增加而減少。瘤胃總VFA隨日糧NFC/NDF的增加而增加,其中乙酸的濃度隨NFC/NDF增加而降低,丙酸和丁酸的濃度增加。分子實(shí)驗(yàn)結(jié)果表明：各組間MCT1、PAT1、 vH+ATPase等基因的表達(dá)量沒(méi)有顯著影響(P0.05)。NHE3和DRA的表達(dá)量隨日糧NFC的增加而增加。MCT4的表達(dá)量反而隨日糧NFC的增加而降低。NHE1和Na+、K+ATPase的表達(dá)量在NCF/NDF為0.66時(shí)最高,而NFC/NDF過(guò)低或過(guò)高都會(huì)降低其表達(dá)量。試驗(yàn)2日糧不同NFC/NDF和RDP對(duì)對(duì)細(xì)毛羊消化代謝、瘤胃內(nèi)環(huán)境及SCFA吸收和氮素轉(zhuǎn)運(yùn)的影響選用12只體況良好,體重為44.2±4.35Kg有永久性瘤胃瘺管的內(nèi)蒙古鄂爾多斯細(xì)毛羊半同胞羯羊,按體重分為4組：1組,NFC/NDF:1.14, CP:15.5：2組,NFC/NDF:1.14,CP：10.5；3組NFC/NDF:0.35,CP：15.5：4組,NFC/NDF:0.35,CP：10.5。飼養(yǎng)管理同試驗(yàn)l。結(jié)果表明,細(xì)毛羊氮代謝受日糧RDF的影響,隨日糧RDP的增加而增加。而各組間糞氮和糞NDF含量無(wú)顯著差異。瘤胃PH隨日糧NFC/NDF的增加而降低。瘤胃NH3-N的濃度隨日糧RDP的增加而增加,并且日糧NFC/NDF低時(shí)NH3-N濃度明顯增加。日糧NFC/NDF和RDP對(duì)NHE1、Na+、K+ATPase和H+ATPase的表達(dá)量無(wú)顯著影響(P0.05)。NFC/NDF和RPD都相對(duì)較高時(shí),MCTl和MCT4的表達(dá)量最高。NHE3的表達(dá)量隨NFC/NDF的增加而增加,受日糧RDP的影響。日糧NFC/NDF相同時(shí),低N日糧可增加UT-B的表達(dá)量。日糧蛋白水平相同時(shí),低NFC/NDF組的UT-B表達(dá)量增加。日糧NFC/NDF為較高(1.14)時(shí),水通道蛋白的表達(dá)量隨日糧蛋白的增加而降低,而日糧NFC/NDF為較低(0.35)時(shí),水通道蛋白的表達(dá)量隨日糧蛋白的增加而增加。日糧NFC/NDF為較高(1.14)時(shí),氨轉(zhuǎn)運(yùn)蛋白的表達(dá)隨日糧蛋白的增加而降低,日糧NFC/NDF為較低(0.35)時(shí),氨轉(zhuǎn)運(yùn)蛋白表達(dá)量隨日糧蛋白的增加而增加。試驗(yàn)3限飼及之后的恢復(fù)對(duì)瘤胃內(nèi)環(huán)境和血液生化指標(biāo)的影響試驗(yàn)動(dòng)物、分組和飼養(yǎng)管理同試驗(yàn)1。結(jié)果表明,總VFA的濃度在63.68-89.49mmol/L之間,隨日糧NFC/NDF的增加而增加。乙酸的濃度在48.35-65.75之間mmol/L,隨日糧NFC/NDF的增加而減少。除11：00以外,乙酸和丙酸濃度各組之間無(wú)顯著差異。各組間NH3-N的濃度無(wú)顯著差異(P0.05)。而pH只隨日糧NFC/NDF的增加而顯著降低。限飼之后突然的恢復(fù)日糧將破壞瘤胃內(nèi)環(huán)境,使瘤胃pH降低。
[Abstract]:The regulation of VFA absorption related gene expression and nitrogen transport in ruminal epithelium of Inner Mongolia fine wool sheep was investigated by feeding experiments, fluorescence quantitative and Western blot methods. The contents and results of this paper were as follows: the digestion and metabolism of different NFC/NDF to fine wool sheep in the 1 day diet and the rumen in the rumen were tested in this paper. The effects of environment and SCFA absorption were divided into 9 half sib sheep of Inner Mongolia Ordos fine wool sheep with a good body weight of 43.8 + 4.75Kg and a permanent rumen fistula. The body weight was divided into 3 groups: NFC/NDF, 0.35,0.66 and 1.14, the protein level was the same, and the 13.5%. feeding test table NFC/NDF had no significant effect on the nitrogen metabolism of fine wool sheep. The NDF in the feces decreased with the increase of dietary NFC/NDF. The total VFA of the rumen increased with the increase of dietary NFC/NDF, and the concentration of acetic acid decreased with the increase of NFC/NDF, and the concentration of propionic acid and butyric acid increased. The results of molecular experiments showed that the expression of MCT1, PAT1, vH+ATPase and other genes in each group had no significant influence on the expression of.NHE3 and DRA. With the increase of dietary NFC, the expression of.MCT4 decreased with the increase of NFC in diet and decreased.NHE1 and Na+. The expression of K+ATPase was the highest when NCF/NDF was 0.66, while NFC/NDF was too low or too high to reduce its expression. Experimental 2 diet was different from NFC/NDF and RDP to the digestion and metabolism of fine wool sheep, in the rumen environment and SCFA absorption and nitrogen transport. The 12 half sib sheep of Inner Mongolia Ordos fine wool sheep with a good body weight of 44.2 + 4.35Kg and a permanent rumen fistula were divided into 4 groups according to body weight: 1 groups, NFC/NDF:1.14, CP:15.5:2, NFC/NDF:1.14, CP:10.5; 3 groups of NFC/NDF:0.35, CP:15.5:4 group, NFC/NDF: 0.35, CP:10.5. feeding management and L. results showed that L. results were found, The nitrogen metabolism of fine wool sheep was affected by dietary RDF and increased with the increase of dietary RDP. But there was no significant difference in the content of fecal nitrogen and fecal NDF between each group. The rumen PH decreased with the increase of dietary NFC/NDF. The concentration of NH3-N in the rumen increased with the increase of dietary RDP, and the concentration of NH3-N increased obviously when the diet NFC/NDF was low. NFC/NDF and RDP on the diet were for NHE1 and RDP. The expression of ASE and H+ATPase was not significantly affected (P0.05).NFC/NDF and RPD were relatively high, the highest expression of MCTl and MCT4 increased with the increase of NFC/NDF, and was influenced by RDP in the diet. When dietary NFC/NDF was higher (1.14), the expression of water channel protein decreased with the increase of dietary protein, while the expression of water channel protein increased with the increase of dietary protein when NFC/NDF was lower (0.35). When dietary NFC/NDF was higher (1.14), the expression of ammonia transporter decreased with the increase of dietary protein, and NFC/NDF in diet. At lower (0.35), the expression of ammonia transporter increased with the increase of dietary protein. Experimental animals, group and feeding management and test 1. showed that the effects of 3 limited feeding and recovery on the rumen environment and blood biochemical indexes showed that the concentration of total VFA was between 63.68-89.49mmol/ L and increased with the increase of dietary NFC/NDF. The concentration of mmol/L decreased with the increase of NFC/NDF in the diet. There was no significant difference between the concentrations of acetic acid and propionic acid except at 11:00. There was no significant difference between the concentrations of acetic acid and propionic acid. The concentration of NH3-N in each group was not significantly different (P0.05). But the pH only decreased with the increase of NFC/NDF in the diet. The ruminal pH was destroyed by the sudden recovery of the rumen environment after the limited feeding. Lower.
【學(xué)位授予單位】：內(nèi)蒙古農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S826.5
本文編號(hào)：2118793
[Abstract]:The regulation of VFA absorption related gene expression and nitrogen transport in ruminal epithelium of Inner Mongolia fine wool sheep was investigated by feeding experiments, fluorescence quantitative and Western blot methods. The contents and results of this paper were as follows: the digestion and metabolism of different NFC/NDF to fine wool sheep in the 1 day diet and the rumen in the rumen were tested in this paper. The effects of environment and SCFA absorption were divided into 9 half sib sheep of Inner Mongolia Ordos fine wool sheep with a good body weight of 43.8 + 4.75Kg and a permanent rumen fistula. The body weight was divided into 3 groups: NFC/NDF, 0.35,0.66 and 1.14, the protein level was the same, and the 13.5%. feeding test table NFC/NDF had no significant effect on the nitrogen metabolism of fine wool sheep. The NDF in the feces decreased with the increase of dietary NFC/NDF. The total VFA of the rumen increased with the increase of dietary NFC/NDF, and the concentration of acetic acid decreased with the increase of NFC/NDF, and the concentration of propionic acid and butyric acid increased. The results of molecular experiments showed that the expression of MCT1, PAT1, vH+ATPase and other genes in each group had no significant influence on the expression of.NHE3 and DRA. With the increase of dietary NFC, the expression of.MCT4 decreased with the increase of NFC in diet and decreased.NHE1 and Na+. The expression of K+ATPase was the highest when NCF/NDF was 0.66, while NFC/NDF was too low or too high to reduce its expression. Experimental 2 diet was different from NFC/NDF and RDP to the digestion and metabolism of fine wool sheep, in the rumen environment and SCFA absorption and nitrogen transport. The 12 half sib sheep of Inner Mongolia Ordos fine wool sheep with a good body weight of 44.2 + 4.35Kg and a permanent rumen fistula were divided into 4 groups according to body weight: 1 groups, NFC/NDF:1.14, CP:15.5:2, NFC/NDF:1.14, CP:10.5; 3 groups of NFC/NDF:0.35, CP:15.5:4 group, NFC/NDF: 0.35, CP:10.5. feeding management and L. results showed that L. results were found, The nitrogen metabolism of fine wool sheep was affected by dietary RDF and increased with the increase of dietary RDP. But there was no significant difference in the content of fecal nitrogen and fecal NDF between each group. The rumen PH decreased with the increase of dietary NFC/NDF. The concentration of NH3-N in the rumen increased with the increase of dietary RDP, and the concentration of NH3-N increased obviously when the diet NFC/NDF was low. NFC/NDF and RDP on the diet were for NHE1 and RDP. The expression of ASE and H+ATPase was not significantly affected (P0.05).NFC/NDF and RPD were relatively high, the highest expression of MCTl and MCT4 increased with the increase of NFC/NDF, and was influenced by RDP in the diet. When dietary NFC/NDF was higher (1.14), the expression of water channel protein decreased with the increase of dietary protein, while the expression of water channel protein increased with the increase of dietary protein when NFC/NDF was lower (0.35). When dietary NFC/NDF was higher (1.14), the expression of ammonia transporter decreased with the increase of dietary protein, and NFC/NDF in diet. At lower (0.35), the expression of ammonia transporter increased with the increase of dietary protein. Experimental animals, group and feeding management and test 1. showed that the effects of 3 limited feeding and recovery on the rumen environment and blood biochemical indexes showed that the concentration of total VFA was between 63.68-89.49mmol/ L and increased with the increase of dietary NFC/NDF. The concentration of mmol/L decreased with the increase of NFC/NDF in the diet. There was no significant difference between the concentrations of acetic acid and propionic acid except at 11:00. There was no significant difference between the concentrations of acetic acid and propionic acid. The concentration of NH3-N in each group was not significantly different (P0.05). But the pH only decreased with the increase of NFC/NDF in the diet. The ruminal pH was destroyed by the sudden recovery of the rumen environment after the limited feeding. Lower.
【學(xué)位授予單位】：內(nèi)蒙古農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S826.5
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