本文選題：白藜蘆醇 + 脂肪肝出血綜合征��； 參考：《江西農(nóng)業(yè)大學(xué)》2017年碩士論文

【摘要】：本試驗旨在研究高能低蛋白日糧誘導(dǎo)的蛋雞脂肪肝臟、腎臟和小腸中自噬、凋亡及相關(guān)基因m RNA表達(dá)量的變化以及日糧中添加白藜蘆醇對其基因表達(dá)量的調(diào)控作用。144羽海蘭褐蛋雞隨機分成正常組(Con)、病理組(HELP)、預(yù)防組(HELP+Res)和白藜蘆醇組(Con+Res)。試驗期為120d,分別于試驗第40d、第80d和第120d剖殺12羽蛋雞,取肝、腎臟及小腸并提取RNA,通過Quantitative Real Time PCR技術(shù)檢測其ATG5、ATG7、Beclin-1和P62及相關(guān)基因Caspase-3、Bax、Bcl-2、HSP60、HSP70、HSP90、apoA-Ⅰ和apoB-100 mRNA表達(dá)量。結(jié)果如下:1、與Con組比,肝、腎臟及小腸中Beclin-1、ATG5和ATG7 mRNA表達(dá)量在HELP組降低,在Con+Res組升高,P62 mRNA則完全相反,均差異極顯著(P0.01);與HELP組比,HELP+Res組各組織中Beclin-1、ATG5和ATG7 mRNA表達(dá)量升高,P62 m RNA降低,均差異極顯著(P0.01)。2、與Con組比,各組織中Caspase-3和Bax mRNA表達(dá)量在HELP組升高,在Con+Res組降低,Bcl-2 mRNA則完全相反,均差異極顯著(P0.01);與HELP組比,HELP+Res組各組織中Caspase-3和Bax mRNA表達(dá)量降低,Bcl-2 mRNA升高,均差異極顯著(P0.01)。3、與Con組比,各組織中HSP60、HSP70和HSP90 mRNA表達(dá)量在HELP組呈升高,均差異顯著(P0.05),在Con+Res組中無明顯差異(P0.05);與HELP組,HELP+Res組各組織中HSP60、HSP70和HSP90 mRNA表達(dá)量降低,均差異顯著(P0.05)。4、與Con組比,各組織apoA-Ⅰ和apoB-100 mRNA表達(dá)量在HELP組降低,在Con+Res組升高,均差異極顯著(P0.01);與HELP組比,HELP+Res組各組織中apoA-Ⅰ和apoB-100表達(dá)量升高,均差異極顯著(P0.01)。高能低蛋白誘導(dǎo)的蛋雞脂肪肝出血綜合征會抑制肝、腎臟及小腸自噬、載脂蛋白基因的表達(dá),促進凋亡基和熱休克基因的表達(dá),日糧中添加白藜蘆醇能對這些基因的表達(dá)進行調(diào)控。
[Abstract]:The aim of this study was to study the effects of high energy and low protein diets on autophagy in fat liver, kidney and small intestine of laying hens. The changes of mRNA expression of apoptosis and related genes and the regulation of resveratrol on the gene expression in diet. 144 layers were randomly divided into normal group (Con), pathological group (HELP), prevention group (HELP Res) and resveratrol group (Con res), and was divided into three groups: normal group (Con), pathological group (HELP), prevention group (HELP Res) and resveratrol group (Con res). Twelve laying hens were killed on the 40th, 80th and 120th day of the experiment, liver, kidney and small intestine were taken and RNAs were extracted. The expressions of ATG5, ATG7, Beclin-1 and P62 and the related genes Caspase-3, Bax-2HSP60HSP70A- 鈪，

本文編號：2110708