本文選題：乳腺炎 + 鑄型��； 參考：《解剖學(xué)報(bào)》2017年06期

【摘要】：目的揭示金黃色葡萄球菌感染對(duì)泌乳期羊的乳腺導(dǎo)管及腺泡的三維立體構(gòu)造的影響。方法選取3頭產(chǎn)后母羊并建立羊乳腺炎模型,在羊左側(cè)乳腺注射濃度為1012CFU/L的金黃色葡萄球菌1 ml,右側(cè)乳腺作為對(duì)照,注入1 ml生理鹽水,羊的左側(cè)乳腺在攻菌12 h、24 h、36 h、48 h后,觀察其臨床癥狀。使用奶牛隱性乳房炎快速診斷技術(shù)(BMT)確認(rèn)乳腺炎模型構(gòu)造成功,取下乳腺后運(yùn)用導(dǎo)管鑄型技術(shù)獲得羊乳腺導(dǎo)管、腺泡的鑄型標(biāo)本,利用掃描電子顯微鏡技術(shù),檢測(cè)乳腺小導(dǎo)管及腺泡的微觀結(jié)構(gòu)。結(jié)果鑄型標(biāo)本結(jié)果顯示,以輸乳管為第1級(jí),最多可達(dá)14級(jí)分支,導(dǎo)管分支間未見(jiàn)吻合現(xiàn)象,各級(jí)分支導(dǎo)管與上一級(jí)導(dǎo)管主軸延長(zhǎng)線的夾角90°。經(jīng)掃描電子顯微鏡觀察發(fā)現(xiàn),正常乳腺導(dǎo)管末梢小導(dǎo)管管壁光滑,末端多呈膨大球狀,腺泡直徑(79.03±14.91)μm,金黃色葡萄球菌感染的乳腺導(dǎo)管表面有凹陷,末端大部分腺泡萎縮,腺泡直徑(42.89±17.17)μm。結(jié)論金黃色葡萄球菌感染能破壞乳腺腺泡的正常結(jié)構(gòu)。
[Abstract]:Objective to investigate the effect of Staphylococcus aureus infection on the three dimensional structure of mammary duct and acinus in lactating sheep. Methods Sheep mastitis models were established in 3 postpartum ewes. Staphylococcus aureus was injected with 1012 CFU / L Staphylococcus aureus in the left mammary gland of the sheep and 1 ml of normal saline was injected into the right mammary gland as control. The clinical symptoms were observed. The successful mastitis model was confirmed by the rapid diagnosis technique (BMT) of cow recessive mastitis. After the mammary gland was removed, the goat mammary duct and the cast specimen of the acinus were obtained using the technique of scanning electron microscope. The microstructures of mammary ducts and acinus were examined. Results the results of the cast specimen showed that the breast feeding tube was the first grade and the maximum was 14 grade branches. There was no anastomosis between the ductal branches. The angle between the ducts of each grade and the extension line of the main shaft of the upper grade ducts was 90 擄. The results of scanning electron microscope showed that the wall of the small ducts in the end of the normal mammary ducts was smooth, the ends of the ducts were bulging and the acinus diameter was (79.03 鹵14.91) 渭 m. The surface of the ducts infected with Staphylococcus aureus was depressed, and most of the acinus shrank at the end of the ducts. The acinar diameter was (42.89 鹵17.17) 渭 m. Conclusion Staphylococcus aureus infection can destroy the normal structure of mammary acinus.
【作者單位】： 黑龍江八一農(nóng)墾大學(xué)動(dòng)物科技學(xué)院;
【基金】：黑龍江省大學(xué)生創(chuàng)新創(chuàng)業(yè)項(xiàng)目(201610223072) 黑龍江八一農(nóng)墾大學(xué)博士啟動(dòng)基金(XYB2013-11)
【分類號(hào)】：S858.27

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