本文選題：豬流行性腹瀉病毒 + 流行病學調查��； 參考：《黑龍江八一農墾大學》2015年碩士論文

【摘要】：豬流行性腹瀉（porcineepidemicdiarrhea，PED）是由豬流行性腹瀉病毒（porcineepidemicdiarrheavirus，PEDV）引起的豬的一種急性、高度傳染性的腸道疾病。該病毒主要破壞豬的消化道器官，其主要臨床癥狀為急性腸炎、嘔吐、水樣腹瀉和脫水。各年齡段以及不同品系的豬均對PEDV敏感，該病毒對哺育仔豬危害性非常大，其發(fā)病率為100%，死亡率為80~100%，給養(yǎng)豬業(yè)造成了嚴重的經(jīng)濟損失。 2010年末PED在我國華南地區(qū)發(fā)生，隨后在國內廣泛流行，發(fā)病率和死亡率居高不下。為了研究豬流行性腹瀉病毒在中國的流行情況，本課題組于2014年1月至12月對來自9個省份和2個直轄市（黑、吉、遼、內蒙古、冀、魯、晉、蘇、湘、京、津）42個發(fā)生腹瀉豬場的165份腹瀉樣品，通過膠體金和RT-PCR等一系列實驗方法對其進行了檢測，結果顯示，有25個豬場僅檢測出了PEDV，占總數(shù)的59.52%；有11個豬場還檢出了傳染性胃腸炎病毒（TGEV）和豬輪狀病毒（PoRV），占總數(shù)的26.19%。165份被檢測的病料中，病料總數(shù)44.24%的病料單獨感染PEDV，數(shù)量為73份；有55份PEDV、TGEV、PoRV混感的病料，占總數(shù)的33.33%；其余樣品的病原為其他腸道病病原。在42個發(fā)病豬場中，感染PEDV的豬場有36個，占豬場總數(shù)的85.71%；165份病料樣品中，77.6%的病料樣品呈PEDV陽性，數(shù)量為128份。本實驗結果表明：PEDV在我國的豬腹瀉病病原中占主導地位。 根據(jù)獲得的21株ORF3基因檢測結果表明，均由675個核苷酸組成，與GenBank上已登錄的12株國內外參考毒株進行序列比對，同源性為90.6%～100%。與國內外毒株同源性比較結果顯示，，中國目前流行毒株與歐洲毒株和中國較早流行毒株同源性較低，而與韓國毒株同源性較高。33個PEDV毒株的ORF3基因系統(tǒng)發(fā)育樹顯示，中國PEDV現(xiàn)地野毒株和PEDV參考毒株被分為2個群。
[Abstract]:Porcineepidemicdiarrhea (PED) is an acute, highly contagious intestinal disease caused by porcineepidemicdiarrheavirus (PEDV). The virus mainly destroys the digestive tract organs of the pig. Its main clinical symptoms are acute enteritis, vomiting, water like diarrhea and dehydration. The pigs of different strains were sensitive to PEDV, and the virus was very harmful to the piglets, the incidence was 100% and the mortality rate was 80~100%, which caused serious economic loss to the pig industry.
At the end of 2010, PED took place in the Southern China area of China, followed by widespread prevalence in China, and the incidence and mortality rate remained high. In order to study the prevalence of porcine epidemic diarrhea virus in China, the subject group took 42 abdomen from January 2014 to December to 9 provinces and 2 municipalities (black, Ji, Liao, Inner Mongolia, Hebei, Lu, Jin, Su, Xiang, Jing, Tianjin). 165 samples of diarrhoea in diarrhea piggery were tested by a series of experimental methods such as colloid gold and RT-PCR. The results showed that only 25 pig farms were detected only PEDV, accounting for 59.52% of the total, and 11 piggery also detected infectious gastroenteritis virus (TGEV) and porcine rotavirus (PoRV), which accounted for the total number of 26.19%.165 samples. A total of 44.24% of the total diseases were infected with PEDV, with a number of 73 copies, 55 PEDV, TGEV, and PoRV mixed diseases, accounting for 33.33% of the total; the other samples were pathogenic to other intestinal diseases. In 42 swine farms, 36 pigs infected with PEDV, 85.71% of the total pig farm, and 77.6% of the 165 samples of the disease samples were PEDV positive. Sex and quantity were 128 copies. The results showed that PEDV played a leading role in the pathogenesis of swine diarrhea in China.
According to the results of the detection of 21 ORF3 genes, 675 nucleotides were made up and compared with the 12 domestic and domestic reference strains on GenBank. Homology from 90.6% to 100%. and homology of domestic and foreign strains showed that the homology of the current epidemic strains in China and the early epidemic strains of Europe and China were low. The ORF3 gene phylogenetic tree of.33 PEDV strains with high homology with the Korean strain showed that the Chinese PEDV wild strain and PEDV reference strain were divided into 2 groups.
【學位授予單位】：黑龍江八一農墾大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：S858.28

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