發(fā)布時(shí)間：2018-07-07 21:38

  本文選題：驢常見寄生蟲 + 糞檢��； 參考：《新疆農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：近幾年來南疆驢業(yè)發(fā)展迅速,驢寄生蟲病的發(fā)病率呈上升趨勢(shì),嚴(yán)重影響了南疆地區(qū)養(yǎng)殖驢業(yè)的健康發(fā)展。驢常見寄生蟲病包括驢體內(nèi)的蠕蟲病、原蟲病及外寄生蟲病,主要臨床癥狀為貧血、消瘦、黃疸、發(fā)育受阻、發(fā)生各種炎癥或嚴(yán)重的惡疾病至死亡。了解南疆地區(qū)驢常見寄生蟲種類、感染情況,從而針對(duì)性提出綜合防治辦法、降低驢體內(nèi)外常見寄生蟲的發(fā)病率、感染率所在。本研究包括以內(nèi)容:(ⅰ)通過常規(guī)寄生蟲病原學(xué)的檢查方法,對(duì)采自疏勒縣、澤普縣、阿合奇縣、庫車縣、吐魯番市和托克遜縣等6個(gè)實(shí)驗(yàn)點(diǎn)的254頭驢糞樣、215份血樣和22份驢內(nèi)臟進(jìn)行了消化道寄生蟲和梨形蟲的檢查、分析。利用蟲卵漂浮法對(duì)254份糞樣進(jìn)行糞檢和蟲卵的鑒定,結(jié)果顯示,南疆驢寄生蟲的優(yōu)勢(shì)蟲種為馬副蛔蟲、毛細(xì)線蟲、馬圓線蟲(包括馬圓線蟲、無齒圓線蟲、普通圓線蟲等3種)和球蟲,其感染率分別為35%(88"254)、47%(119"254)、65%(165"254)和55%(140"254),其中馬副蛔蟲感染率較高;利用蟲卵計(jì)數(shù)(EPG)的方法,查出了驢消化道寄生蟲每一克糞樣中的感染強(qiáng)度,如馬圓線蟲695個(gè)/g、馬副蛔蟲332個(gè)/g、球蟲289個(gè)/g和毛細(xì)線蟲234個(gè)/g,其中馬圓形線蟲的感染率較高。在6個(gè)采樣點(diǎn)中,采自于疏勒縣的糞樣中,驢體內(nèi)寄生蟲染率可達(dá)60%,高于其它點(diǎn),差異顯著(P0.05);采自于吐魯番市的糞樣中,驢毛細(xì)線蟲感染率可達(dá)52.38%,高于其它點(diǎn),差異顯著(P0.05);采自于6采樣點(diǎn)的糞樣中均有馬副蛔蟲感染,各個(gè)采樣點(diǎn)之間馬副蛔蟲的感染率,差異不顯著(P0.05);采自于托克遜縣的糞樣中,球蟲感染率可達(dá)57.5%,高于其它點(diǎn),差異顯著(P0.05)。利用蠕蟲學(xué)剖檢術(shù)(N=22),發(fā)現(xiàn)驢感染的消化道寄生蟲有8種,隸屬3科3屬,其中線蟲4種、胃蠅4種,馬圓線蟲、無齒圓線蟲、普通圓線蟲、馬副蛔蟲、腸胃蠅、紅尾胃蠅、獸胃蠅和鼻胃蠅等的感染率分別為22.72%、13.64%、9.09%、18.18%、9.09%、4.55%、9.09%、4.55%。經(jīng)改良的血液膜片染色鏡檢法進(jìn)行了驢血液膜片的檢查發(fā)現(xiàn),在215分血樣中檢出驢泰勒蟲陽性為42份,其陽性率為19.53%(42"215)。(ⅱ)通過形態(tài)學(xué)(包括形態(tài)學(xué)顯微和超微觀察)、PCR法及c ELISA方法,對(duì)采自南疆驢體表寄生的蜱蟲(N=432)、蜱傳血液原蟲(N=215血樣)進(jìn)行了調(diào)查、分析,鑒定出了璃眼蜱屬的小亞璃眼蜱213只(雄)和血蜱屬的刻點(diǎn)血蜱219只(雄);經(jīng)PCR方法檢測(cè)出了驢感染的泰勒蟲陽性病例,其感染率為35.81%(77"215),經(jīng)陽性樣品的克隆、測(cè)序比對(duì)分析,驗(yàn)證了其與T.equi標(biāo)準(zhǔn)蟲株的同源性為99.8%。經(jīng)c ELISA檢測(cè)出了驢泰勒蟲抗體,其陽性率為20.93%(45"215),駑巴貝斯蟲的陽性率為1.40%(3"215)。實(shí)驗(yàn)結(jié)果表明該試驗(yàn)區(qū)域存在蜱傳驢泰勒蟲,應(yīng)該加強(qiáng)防范。
[Abstract]:In recent years, the donkey industry in southern Xinjiang has developed rapidly, and the incidence of donkey parasite disease is on the rise, which has seriously affected the healthy development of donkey breeding industry in southern Xinjiang. Common parasitic diseases in donkeys include helminthiasis protozoa and external parasitosis. The main clinical symptoms are anemia wasting jaundice stunted development inflammation or serious diseases to death. In order to reduce the incidence and infection rate of common parasites in and out of donkeys, the comprehensive control measures were put forward in order to understand the common parasitic species and infection status of donkeys in southern Xinjiang. The contents of this study are as follows: (I) collected from Shule County, Zepu County, Aheqi County, Kuqa County through routine parasite etiology examination methods, 215 blood samples of 254 donkey feces and 22 donkey viscera from 6 experimental sites in Turpan City and Tokeson County were examined for digestive tract parasites and piriform worms. The fecal examination and egg identification of 254 feces were carried out by the method of egg floating. The results showed that the dominant species of the parasite of the donkeys in South Xinjiang were Ascaris equalis, Trichoderma capillaris, nematode equina (including the nematodes of the horse, and the nematode without teeth). The infection rate of common nematode and coccidiosis was 35% (88 "FU 254) and 65% (165" T254) and 55% (140 "T254), respectively, and the infection rate of Ascaris equinus was higher. The infection intensity of parasites in the digestive tract of donkey was detected by the method of egg count (EPG). For example, 695 / g of nematode, 332 / g of Ascaris equi, 289 / g / g of coccidia and 234 / g / g of nematode capillaries. In 6 sampling sites, the parasite infection rate of donkey was 60, higher than that of other points (P0.05), the infection rate of Donkey nematode reached 52.38, which was higher than that of other points, in the feces collected from 6 sampling sites in Shule County, while in the feces of Turpan City, the infection rate was 52.38%, which was higher than that in other points. The difference was significant (P0.05), the infection rate of Ascaris equis was not significant among the different sampling sites (P0.05), the infection rate of coccidia reached 57.5% in fecal samples collected from Toksonian County, which was higher than that of other sites. The difference was significant (P0.05). By worm dissecting technique (NN22), we found that there are 8 species of digestive tract parasites infected by donkey, belonging to 3 families and 3 genera, including 4 species of nematodes, 4 species of gastric flies, 4 species of nematode, 4 species of nematode, 2 species of Ascaris equalis, 3 species of gastroenteric fly and 4 species of red stomach fly. The infection rates of stomach fly and stomach fly were 22.72 and 13.64 and 9.09, respectively. The infection rate was 18.189.09 and 4.559.09. The improved blood membrane staining method was used to examine the blood membrane of the donkey. 42 of 215 blood samples were found to be positive for Taylor's disease. The positive rate was 19.53% (42 "~ 215). (鈪，

本文編號(hào)：2106353