本文選題：PPRV + 小反芻獸疫　； 參考：《中國(guó)獸醫(yī)學(xué)報(bào)》2017年05期

【摘要】：應(yīng)用表達(dá)純化的小反芻獸疫病毒(peste des petits ruminants virus,PPRV)N蛋白制備的多克隆抗體,建立了檢測(cè)PPRV抗原的雙抗體夾心ELISA方法,并對(duì)吉林省和內(nèi)蒙古地區(qū)羊群感染PPRV進(jìn)行了調(diào)查。方陣法確定了抗PPRV兔源IgG作為捕獲抗體的包被量為0.2μg,酶標(biāo)抗體的最佳稀釋度為1∶1 000。對(duì)大量小反芻獸疫陰性糞便樣品進(jìn)行檢測(cè)及統(tǒng)計(jì)學(xué)處理,確定了雙抗體夾心ELISA檢測(cè)PPRV的判定標(biāo)準(zhǔn),即被檢糞便樣品D490≥0.221,判定為陽(yáng)性。特異性、敏感性等試驗(yàn)結(jié)果表明,建立的檢測(cè)PPRV抗原方法具有特異、敏感和快速等優(yōu)點(diǎn)。與RT-PCR方法相比,該方法省時(shí)省力、簡(jiǎn)單快速。應(yīng)用建立的檢測(cè)PPRV抗原的雙抗體夾心ELISA對(duì)吉林省和內(nèi)蒙古不同地區(qū)的羊糞樣進(jìn)行檢測(cè),發(fā)現(xiàn)羊群均存在程度不同的PPRV隱性感染。本研究在國(guó)內(nèi)首次揭示出臨床健康羊群攜帶PPRV,為今后小反芻獸疫的診斷與防控提供了新的流行病學(xué)理論依據(jù)。
[Abstract]:A double antibody sandwich Elisa method was developed to detect (peste des petits ruminants virus antigen by using polyclonal antibody prepared from purified protein of small ruminant virus (peste des petits ruminants virus) N. The infection of sheep in Jilin province and Inner Mongolia was investigated. The anti-PPRV rabbit IgG was determined by square matrix method as the encapsulation amount of the capture antibody was 0.2 渭 g, and the best dilution of the enzyme labeled antibody was 1:1 000. A large number of small ruminant negative fecal samples were detected and statistically analyzed, and the standard of double antibody sandwich Elisa for PPRV detection was determined, that is, D490 鈮，

本文編號(hào)：2106162