本文選題：蒙古馬 + 高負荷運動�。� 參考：《畜牧獸醫(yī)學(xué)報》2017年06期

【摘要】：旨在對蒙古馬高負荷運動訓(xùn)練前后轉(zhuǎn)錄組差異表達進行分析。本試驗對6匹進行了4個月高負荷運動訓(xùn)練的蒙古馬進行研究,分別在訓(xùn)練前后兩個時期采集肌肉樣品,利用二代測序技術(shù)對肌肉樣品建立轉(zhuǎn)錄組文庫,對發(fā)生顯著變化的差異基因進行GO功能富集和KEGG Pathway分析,尋找訓(xùn)練前后發(fā)生顯著變化的代謝通路及相關(guān)候選基因。結(jié)果表明,在轉(zhuǎn)錄組篩選到的1 102個顯著差異表達基因中,有299個在訓(xùn)練后發(fā)生下調(diào),803個發(fā)生上調(diào)。這些差異基因被注釋到3 398條GO term上。其中與運動學(xué)相關(guān)的生物學(xué)過程有:肌肉結(jié)構(gòu)發(fā)育、心血管系統(tǒng)發(fā)育、循環(huán)系統(tǒng)發(fā)育、肌肉組織發(fā)育、肌肉器官發(fā)育、肌肉收縮和肌肉細胞分化等。對差異表達基因進行KEGG pathway分析,結(jié)果表明,差異基因被富集到如擴張型心肌病、肥厚型心肌病、心肌收縮、鈣信號通路、肌動蛋白骨架調(diào)節(jié)等與運動相關(guān)的193條通路中。本研究結(jié)果為探究蒙古馬強耐力的基本分子機理提供理論基礎(chǔ),同時也為理解蒙古馬運動學(xué)特性提供新的思路。
[Abstract]:The purpose of this study was to analyze the differential expression of transcriptome in Mongolian horse before and after high-load exercise training. In this experiment, six Mongolian horses trained for 4 months with high-load exercise were studied. Muscle samples were collected before and after training, and the transcriptional library of muscle samples was established by second-generation sequencing technique. Go functional enrichment and KEGG Pathway analysis were performed to identify the metabolic pathways and related candidate genes that changed significantly before and after training. The results showed that 299 of the 1 102 differentially expressed genes were up-regulated after training. These differential genes were annotated to 3 398 go term. The biological processes related to kinematics include muscle structure development, cardiovascular system development, circulatory system development, muscle tissue development, muscle organ development, muscle contraction and muscle cell differentiation. The differentially expressed genes were analyzed by KEGG pathway. The results showed that the differentially expressed genes were enriched into 193 exercise-related pathways such as dilated cardiomyopathy, hypertrophic cardiomyopathy, myocardial contraction, calcium signaling pathway and actin skeleton regulation. The results of this study provide a theoretical basis for exploring the basic molecular mechanism of Mongol horse's strong endurance, and also provide a new idea for understanding the kinematic characteristics of Mongolian horse.
【作者單位】： 內(nèi)蒙古農(nóng)業(yè)大學(xué)動物科學(xué)學(xué)院內(nèi)蒙古自治區(qū)蒙古馬遺傳資源保護及馬產(chǎn)業(yè)工程實驗室;
【基金】：國家自然科學(xué)基金(31360538;31472070) 內(nèi)蒙古自治區(qū)科技廳重點實驗室建設(shè)項目(20130902) 內(nèi)蒙古自治區(qū)高等學(xué)校科學(xué)研究一般項目(NJZY0701)
【分類號】：S821.2
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本文編號：2102401