本文選題：無乳鏈球菌 + 停乳鏈球菌��； 參考：《內蒙古農業(yè)大學》2015年碩士論文

【摘要】：目的：通過體外誘導細菌產生耐藥性,分析誘導前后細菌耐藥基因與毒力的變化,探討耐藥基因與毒力之間的關系,從而為特定耐藥病原體專用新藥的研發(fā)提供科學依據(jù),亦為鏈球菌產生耐藥性的風險評估提供試驗基礎。方法：本試驗采用微量肉湯稀釋法測定抗微生物藥對細菌的最小抑菌濃度(MIC)；采用體外逐漸增加藥物濃度的方法對標準菌株無乳鏈球菌CVCC1886和停乳鏈球菌CVCC3701進行耐藥性誘導；采用PCR擴增技術對誘導耐藥前后的菌株進行耐藥基因和毒力基因的檢測；采用形態(tài)學觀察法對誘導前后菌株的生理生化性質進行對比；通過測定受試菌株對小鼠的LD50比較誘導前后菌株的毒力變化。結果：1、經(jīng)體外誘導,無乳鏈球菌CVCC1886和停乳鏈球菌CVCC3701對7種抗菌藥均達到了耐藥水平；2、經(jīng)PCR檢測,除青霉素誘導的停乳鏈球菌CVCC3701在誘導后耐藥基因和毒力基因均發(fā)生了增加外,用其他抗菌藥誘導前后菌株的耐藥基因和毒力基因均無變化；3、經(jīng)形態(tài)學觀察表明CVCC3701-青霉素的生長繁殖速度明顯低于停乳鏈球菌CVCC3701的生長繁殖速度；4、小鼠毒力試驗表明誘導前停乳鏈球菌CVCC3701和誘導后CVCC3701-青霉素的LD50值分別為5.45x106CFU/mL、5.82x108CFU/mL,誘導后的毒力較誘導前減弱了1.1×102倍,誘導后CVCC3701-青霉素菌株的毒力明顯低于誘導前的停乳鏈球菌CVCC3701。
[Abstract]:Objective: to investigate the relationship between drug resistance genes and virulence by inducing drug resistance of bacteria in vitro, and to provide scientific basis for the development of new drugs for specific drug resistant pathogens. It also provides experimental basis for risk assessment of streptococcus resistance. Methods: the minimal inhibitory concentration (MIC) of antimicrobial agents to bacteria was determined by broth dilution method, and the drug resistance of standard strains CVCC1886 and CVCC3701 was induced by increasing drug concentration in vitro. PCR amplification technique was used to detect the resistance genes and virulence genes of the strains before and after induction of drug resistance, and morphological observation was used to compare the physiological and biochemical properties of the strains before and after induction. The toxicity of the tested strain to LD50 in mice was compared before and after induction. Results: after in vitro induction, both Streptococcus actinomycis CVCC1886 and Streptococcus punctatus CVCC3701 reached the level of resistance to 7 antimicrobial agents, which were detected by PCR. The resistance genes and virulence genes of Streptococcus deltostans CVCC3701 induced by penicillin were increased, but the resistance genes and virulence genes were not changed before and after induced by other antimicrobial agents. 3. Morphological observation showed that the growth and reproduction rate of CVCC3701-penicillin was obviously lower than that of Streptococcus acuminatum CVCC3701. 4. The virulence test of mice showed that the LD50 values of CVCC3701 and CVCC3701-penicillin were 5.45x106CFU / mL5.82x108CFU / mLrespectively, the virulence of induced streptococci CVCC3701-penicillin was 1.1 脳 102.The virulence of induced strain CVCC3701-penicillin was obviously lower than that of CVCC3701before induction.
【學位授予單位】：內蒙古農業(yè)大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：S852.61

【參考文獻】

相關期刊論文 前10條

1 劉厚明,何林,周克元;抗生素敏感試驗方法學的研究進展[J];國外醫(yī)學.臨床生物化學與檢驗學分冊;2005年09期

2 黃怡;常用抗菌藥物耐藥機制及對策[J];國外醫(yī)藥(抗生素分冊);1999年01期

3 朱勝金;楊丹;;肺炎鏈球菌耐藥基因及其分子生物學檢測[J];貴州醫(yī)藥;2011年01期

4 高璐琰;楊鐘;武瑞兵;鄭武江;;細菌耐藥性研究進展[J];中國畜牧獸醫(yī);2013年07期

5 蔡麗娟;朱麗敏;劉凱;許寶青;林啟存;;低劑量四環(huán)素誘導嗜水氣單胞菌產生耐藥性的分析[J];安徽農業(yè)科學;2013年17期

6 耿健;李紹雪;周維;張德顯;劉明春;;細菌毒力與β-內酰胺類抗生素耐藥的相關性[J];動物醫(yī)學進展;2014年09期

7 姜成剛,佟恒敏,李艷華;大環(huán)內酯類藥物對鏈球菌耐藥性及耐藥機制[J];黑龍江畜牧獸醫(yī);2005年03期

8 胡子鑒,張嬰元;耐氟喹諾酮類大腸埃希菌的感染與寄殖[J];中國抗感染化療雜志;2002年01期

9 李穎;楊帆;;細菌的耐藥性與適應性[J];中國感染與化療雜志;2010年01期

10 張濤;馬筱玲;戴媛媛;魯懷偉;陳多炎;;體外誘導的萬古霉素耐藥金黃色葡萄球菌耐藥基因檢測[J];臨床檢驗雜志;2011年05期

相關碩士學位論文 前1條

1 吳迪;豬鏈球菌2型分離鑒定及其對免疫抑制小鼠的致病力比較[D];浙江大學;2009年

，

本文編號：2097753