本文選題：梅花鹿 + 鹿茸　； 參考：《畜牧獸醫(yī)學(xué)報(bào)》2017年02期

【摘要】：本研究旨在探究不同生長(zhǎng)時(shí)期梅花鹿鹿茸的轉(zhuǎn)錄組差異,豐富梅花鹿鹿茸轉(zhuǎn)錄組信息。選取5個(gè)生長(zhǎng)時(shí)期(10、20、28、44、66d)的梅花鹿鹿茸組織作為試驗(yàn)樣品,利用Illumina HiSeqTM2500高通量測(cè)序,并用測(cè)序評(píng)估、基因注釋等生物信息學(xué)方法進(jìn)行分析。結(jié)果,經(jīng)過(guò)測(cè)序、轉(zhuǎn)錄本拼接獲得了375 657條contigs,平均長(zhǎng)度為688bp,329 946個(gè)unigenes,平均長(zhǎng)度為534bp。通過(guò)比對(duì)Nr、Nt、Pfam、KOG/COG、Swiss-prot、KEGG、GO等7大數(shù)據(jù)庫(kù),90.07%unigenes得到了成功注釋。其中39 674個(gè)(12.02%)基因成功注釋到GO數(shù)據(jù)庫(kù),在level 2水平上共分為41個(gè)類(lèi)別;17 732個(gè)(5.37%)基因成功注釋到將KOG的26個(gè)類(lèi)別中。對(duì)5個(gè)生長(zhǎng)時(shí)期的鹿茸轉(zhuǎn)錄本文庫(kù)進(jìn)行比較分析,共發(fā)現(xiàn)了509個(gè)差異基因,其中407個(gè)差異表達(dá)基因成功注釋到GO數(shù)據(jù)庫(kù)中,主要為信號(hào)轉(zhuǎn)導(dǎo)、氧化還原、轉(zhuǎn)錄調(diào)節(jié)、蛋白酶降解等生物學(xué)過(guò)程。其中轉(zhuǎn)錄調(diào)節(jié)基因PER1、EGR1的表達(dá)隨著鹿茸的生長(zhǎng)而增加,而GAS1則相反,隨著鹿茸的生長(zhǎng)而降低,推測(cè)PER1、EGR1、GAS1等轉(zhuǎn)錄因子在鹿茸生長(zhǎng)過(guò)程中可能起著重要的調(diào)節(jié)作用。本研究利用高通量測(cè)序技術(shù)對(duì)不同生長(zhǎng)時(shí)期的梅花鹿鹿茸組織進(jìn)行了轉(zhuǎn)錄組測(cè)序和分析,篩選到了梅花鹿鹿茸在不同生長(zhǎng)時(shí)期下的差異表達(dá)基因,并獲得了差異表達(dá)基因的功能。
[Abstract]:The purpose of this study was to explore the transcriptional diversity of sika deer antler at different growth stages, and to enrich the information of sika deer antler transcriptome. Five sika deer antler tissues were selected as experimental samples. Illumina HiSeqTM2500 high-throughput sequencing method was used to analyze sika deer antler tissues. The results were analyzed by sequencing evaluation, gene annotation and other bioinformatics methods. Results: after sequencing, 375,657 contigswere obtained, with an average length of 688bp329,946 unigenes and an average length of 534bp. A successful annotation was obtained by comparing seven databases, such as NrNtPfamKOGR Swiss-protprotgo, KEGGG go and other 7 databases, such as 90.07unigenes. 39,674 (12.02%) genes were successfully annotated into go database, and a total of 17,732 (5.37%) genes were successfully annotated into 26 categories of KOG at level 2 level. A total of 509 differentially expressed genes were found in 5 matured velvet antler transcripts, of which 407 differentially expressed genes were successfully annotated into go database, mainly for signal transduction, redox and transcription regulation. Protease degradation and other biological processes. The expression of transcriptional regulatory gene PER1-EGR1 increased with the growth of velvet antler, whereas GAS1 decreased with the growth of velvet antler, suggesting that transcription factors such as PER1-EGR1-GAS1 might play an important role in the growth of velvet antler. In this study, high-throughput sequencing technique was used to sequence and analyze the transcription patterns of sika deer antler tissues at different growth stages, and the differentially expressed genes of sika deer antler at different growth stages were screened. The function of differentially expressed genes was obtained.
【作者單位】： 中國(guó)農(nóng)業(yè)科學(xué)院特產(chǎn)研究所特種經(jīng)濟(jì)動(dòng)物分子生物重點(diǎn)實(shí)驗(yàn)室;
【基金】：基金項(xiàng)目:特種動(dòng)物遺傳資源創(chuàng)新團(tuán)隊(duì)(CAAS-ASTIP-201X-ISAPS)
【分類(lèi)號(hào)】：S825

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