本文選題：枯草芽孢桿菌 + 耐酸試驗��； 參考：《山東農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：抗生素被廣泛應(yīng)用于養(yǎng)殖業(yè)中,作為添加劑添加到動物飼料中,用以抑制腸道致病菌感染和促進(jìn)動物生長。但是抗生素的使用會導(dǎo)致病原菌和非病原菌的耐藥,而且這些耐藥細(xì)菌可以在動物之間傳播或進(jìn)入環(huán)境中,污染環(huán)境和形成對人類的感染威脅,以及臨床用藥選藥的困難。不容忽視的是,畜禽抗生素的添加使用,可能會導(dǎo)致抗生素性繼發(fā)性感染的問題。專業(yè)人員和養(yǎng)殖者已經(jīng)認(rèn)識到抗生素的使用帶來諸多的問題,在我國養(yǎng)殖業(yè)已經(jīng)進(jìn)入了后抗生素時代,微生態(tài)制劑是首選的替代物。微生態(tài)制劑因其兼具抑制致病菌和促進(jìn)生長的雙重功效,同時又綠色安全,不會造成細(xì)菌耐藥,成為了良好的抗生素替代品,展現(xiàn)出遠(yuǎn)大的前景。本研究將多株不同來源的枯草芽孢桿菌進(jìn)行橫向比較,篩選出性能優(yōu)秀的菌株。首先在動物體外從生長性能、耐酸試驗、耐膽鹽試驗、抑菌試驗、抗生素敏感試驗和血凝試驗等多個方面來比較它們的益生菌潛力,再將表現(xiàn)較好的菌株進(jìn)行動物試驗,檢驗其安全性以及作為假定益生菌的活性效果。結(jié)果顯示,枯草芽孢桿菌B5株生長性能良好,在pH=3.0條件下存活率在90%以上,在pH=1.5時存活率仍然超過70%,且在0.3%的高濃度膽鹽環(huán)境中1h后生存率超過60%。該菌對多株致病菌均表現(xiàn)出良好的抑制效果,對幾種常見抗生素均沒有表現(xiàn)耐藥性。制成凍干粉制劑進(jìn)行動物試驗喂服家兔后提高了實(shí)驗兔的生長性能,所以被認(rèn)定為良好的益生菌株。本研究從多株枯草芽孢桿菌中成功篩選出一株表現(xiàn)優(yōu)異的菌株,并進(jìn)行了動物試驗,為進(jìn)一步研究將這株枯草芽孢桿菌推廣應(yīng)用到養(yǎng)兔業(yè)中打下了基礎(chǔ)。
[Abstract]:Antibiotics are widely used as additives in animal feed to inhibit the infection of intestinal pathogens and promote the growth of animals. However, the use of antibiotics can lead to drug resistance of pathogens and non-pathogenic bacteria, and these drug-resistant bacteria can spread or enter the environment between animals, pollute the environment and form the threat of infection to human beings, as well as the difficulty of clinical drug selection. It can not be ignored that the addition of antibiotics in livestock and poultry may lead to the problem of antibiotic secondary infection. Professionals and breeders have realized that the use of antibiotics has brought a lot of problems. In our country, microecological agents are the first choice substitute in the post-antibiotic era. Because of its dual efficacy of inhibiting pathogenic bacteria and promoting growth, microecological agents can not cause bacterial drug resistance, so they have become a good substitute for antibiotics, showing a broad prospect. In this study, several strains of Bacillus subtilis from different sources were compared horizontally and the strains with excellent performance were screened out. First, the probiotic potential of animals was compared in vitro from growth performance, acid tolerance test, bile salt tolerance test, bacteriostatic test, antibiotic sensitivity test and hemagglutination test. Its safety and the activity of the hypothetical probiotics were tested. The results showed that the growth performance of Bacillus subtilis B5 strain was good, the survival rate of Bacillus subtilis strain B5 was more than 90% at pH 3.0, and still exceeded 70% at pH = 1.5, and the survival rate exceeded 60% after 1 hour in 0.3% high concentration of bile salt. The bacteria showed good inhibitory effect on many pathogenic bacteria, and showed no resistance to several common antibiotics. The freeze-dried powder was used in animal experiment to improve the growth performance of rabbits, so it was considered as a good probiotic strain. In this study, a strain of Bacillus subtilis (Bacillus subtilis) with excellent performance was successfully screened, and animal experiments were carried out, which laid a foundation for further study on the application of Bacillus subtilis to rabbit breeding.
【學(xué)位授予單位】：山東農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S816.3

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本文編號：2087795